Smurf2 (NM_025481) Mouse Untagged Clone

CAT#: MC221302

Smurf2 (untagged) - Mouse SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smurf2
• Synonyms: 2810411E22Rik; AI558114; AI649275
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221302 representing NM_025481
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAACCCCGGAGGCCGGAGGAACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACT
  TGGTGAAAAAGGATTTTTTCCGTCTTCCTGATCCATTTGCTAAGGTGGTAGTTGATGGTTCTGGGCAGTG
  CCATTCTACAGATACTGTGAAGAACACACTTGATCCAAAGTGGAATCAGCATTATGACCTGTATATCGGA
  AAGTCTGATTCAGTTACGATCAGCGTATGGAATCATAAGAAGATCCATAAGAAGCAAGGTGCTGGATTTC
  TTGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGCCTCAAGGACACTGGTTATCAGAGGTTGGATTT
  ATGCAAACTGGGGCCAAATGACAATGATACAGTTCGAGGACAAATTGTAGTAAGTCTTCAGTCCAGAGAC
  CGAATAGGCACGGGAGGACAAGTTGTGGACTGTAGTCGTTTGTTTGACAATGATTTGCCAGATGGTTGGG
  AAGAAAGGCGGACTGCCTCGGGAAGAATCCAGTATCTAAACCACATCACAAGAACTACACAGTGGGAACG
  CCCAACACGACCGGCGTCAGAATATTCTAGTCCTGGCAGACCCCTCAGCTGCTTTGTGGATGAGAATACT
  CCAATTACTGGAACAAATGGTGCAACGTGTGGACATTCTTCAGACCCCAGACTAGCAGAGAGAAGAGTCA
  GGTCCCAGCGACATAGAAATTACATGAGCAGGACACACTTACACACTCCTCCAGACCTACCGGAAGGCTA
  TGAACAAAGGACAACGCAGCAGGGTCAGGTATATTTCTTACATACTCAGACTGGTGTGAGCACATGGCAT
  GATCCACGAGTGCCTAGGGATCTTAGCAACATCAATTGTGAAGAGCTCGGTCCTTTGCCTCCTGGATGGG
  AGATCCGCAATACCGCAACAGGAAGAGTTTATTTCGTTGACCATAACAACAGAACAACACAATTTACAGA
  TCCCCGGCTCTCTGCTAACTTGCATTTAGTTTTAAATCGTCAGAACCAGTTGAAAGACCAACAGCAACAG
  CAAGTGGTGCCATTGTGTCCCGATGACACTGAGTGTCTGACAGTGCCAAGATACAAGCGAGATTTGGTTC
  AAAAACTAAAAATCTTGCGGCAAGAACTTTCCCAACAGCAGCCTCAAGCTGGCCACTGCCGTATTGAGGT
  GTCTAGGGAAGAGATTTTTGAGGAATCATATAGACAGGTCATGAAAATGAGGCCAAAAGATCTATGGAAG
  CGATTAATGATAAAATTTCGTGGAGAAGAAGGTCTTGACTATGGAGGAGTTGCCAGGGAATGGTTGTATC
  TCCTGTCACATGAAATGTTGAATCCTTACTATGGTCTCTTCCAGTATTCTAGAGATGATATCTACACATT
  GCAGATCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCCACTTTGTTGGACGGATAATG
  GGAATGGCTGTATTCCATGGACATTATATTGACGGTGGCTTCACATTACCTTTTTATAAACAGTTGCTTG
  GGAAGTCAATTACTTTGGATGACATGGAGCTAGTTGACCCAGACCTTCATAATAGTTTAGTGTGGATACT
  TGAAAACGATATCACAGGTGTTCTGGACCATACCTTCTGTGTTGAGCATAATGCATATGGTGAAATAATC
  CAGCATGAACTCAAACCAAATGGCAAAAGTATCCCTGTTACTGAAGAAAATAAAAAAGAATATGTCAGGC
  TTTATGTCAACTGGAGATTTCTCCGAGGCATCGAAGCTCAGTTCCTGGCGCTGCAGAAGGGCTTTAATGA
  AGTCATTCCCCAGCACCTGCTGAAGACATTTGATGAGAAGGAGCTGGAGCTCATTATTTGTGGACTTGGC
  AAAATTGATGTGAGTGACTGGAAGGTCAACACCCGGTTAAAACACTGTACCCCAGACAGCAATGTCGTCA
  AGTGGTTCTGGAAAGCGGTGGAGTTTTTTGATGAAGAGAGACGAGCACGGTTGCTCCAGTTTGTGACAGG
  GTCCTCTCGAGTGCCTCTGCAGGGCTTCAAAGCACTACAAGGTGCTGCAGGCCCACGGCTCTTTACCATA
  CACCAGATTGATGCCTGCACGAACAACTTGCCAAAAGCCCACACTTGCTTCAATCGAATAGACATTCCAC
  CCTATGAAAGCTATGAGAAGCTCTATGAAAAGCTGCTAACGGCCATCGAGGAGACATGTGGCTTTGCTGT
  GGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025481
• Insert Size: 2247 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025481.2, NP_079757.2
• RefSeq Size: 5346 bp
• RefSeq ORF: 2247 bp
• Locus ID: 66313
• UniProt ID: A2A5Z6
• Cytogenetics: 11 E1
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.[UniProtKB/Swiss-Prot Function]