Zyg11b (NM_001033634) Mouse Untagged Clone

CAT#: MC221266

Zyg11b (untagged) - Mouse zyg-ll homolog B (C. elegans) (Zyg11b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zyg11b
• Synonyms: 1110046I03Rik; 2810482G21Rik; D4Mgi23; Gm431; mKIAA1730
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221266 representing NM_001033634
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGGACCAGGCCCACGCAGCCATGGAGGAAGCCTCTCCCTATTCCTTGCTTGACATCTGCTTGA
  GTTTCCTGACCACCAACCTCGAGAAGTTCTGCTCAGCACGCCAAGATGGAACCCTGTGTCTGCAGGAGCC
  TGGGGTGTTCCCACAGGAGGTGGCTGACCGGCTTCTTCAGACTATAGCATTCCACGGTCTCCTGAATGAT
  GGAACTGTGGGAATTTTCAGAGGTAACCAGATGCGCCTAAAGCGAGCTTGCATTCGCAAAGCAAAGATTT
  CCGCTGTTGCTTTCCGGAAAGCCTTTTGCCACCACAAGCTAGTAGAACTTGATGCCACGGGTGTGAATGC
  TGACATCACTATCACAGACATCATAAGTGGCCTTGGCAGTAACAAGTGGATCCAGCAAAATCTTCAGTGC
  TTAGTACTAAACTCATTAACTCTGTCCCTCGAGGATCCATATGAGCGGTGCTTCAGCCGGCTCTCTGGTC
  TTCGAGCTCTGAGCATCACTAATGTGCTCTTTTACAATGAAGATCTGGCTGAAGTTGCTTCGCTGCCTAG
  ACTGGAGAGCCTGGATATTTCTAACACCTCAATCACAGATATCACTGCCCTGCTGGCCTGCAAAGACAGA
  CTCAAGTCTCTCACCATGCACCATCTGAAATGTTTAAAAATGACTACTACCCAGATCCTGGATGTCGTTC
  GGGAGCTAAAACATCTGAACCATCTTGACATCTCAGATGATAAACAGTTCACGTCAGACATAGCCCTTCG
  ATTGTTAGAGCAAAAGGACATCTTGCCCAATCTTGTGTCCCTGGATGTTTCAGGGAGAAAGCATGTGACA
  GACAAAGCTGTCGAAGCCTTTATACAGCAGCGGCCTAGCATGCAGTTCGTAGGGTTGCTGGCTACAGATG
  CTGGATACTCTGAATTCCTTATGGGCAAAGGACACTTGAAGGTCTCTGGAGAAGCCAACGAAACCCAGAT
  TGCAGAGGCGCTGAGGCGGTACAGCGAGCGGGCGTTTTTCGTTAGGGAGGCTTTGTTTCATCTCTTTAGC
  CTGACTCATGTGATGGAGAAAACGAAGCCAGACATTTTAAAGCTTGTGGTTACTGGGATGAGAAACCACC
  CAATGAATTTGCCCGTGCAGCTGGCTGCAAGTGCCTGTGTGTTTAACTTAACTAAGCAGGACCTTGCTTT
  AGGCATGCCTGTCCGGCTCCTCGCTGATGTGACCCATTTGCTGCTCAAGGCCATGGAACATTTTCCTAAT
  CACCAACAGTTACAGAAGAATTGCCTCCTCTCACTTTGCAGTGACCGGATTCTTCAAGATGTTCCATTTA
  ATAGGTTTGAAGCAGCCAAGCTTGTCATGCAGTGGCTTTGCAACCACGAAGATCAGAACATGCAACGCAT
  GGCCGTGGCTATCATTTCCATCCTGGCCGCCAAGCTCTCTACAGAACAAACTGCACAGCTTGGTGCTGAG
  CTCTTCATTGTCAGGCAACTTCTTCAAATAGTGAAGCAGAAAACTAATCAGAATTCCGTGGACACTACGT
  TGAAGTTTACTTTGAGTGCACTTTGGAACCTCACAGATGAGTCCCCAACCACGTGCAGACACTTCATTGA
  AAACCAAGGGCTGGAGCTCTTCATGAGGGTTCTAGAGTCTTTCCCAACTGAGTCATCCATTCAACAAAAG
  GTTCTAGGACTTTTGAACAACATAGCTGAAGTACAAGAGCTGCATTCTGAACTAATGTGGAAGGATTTTA
  TAGACCACATCAGCAGTCTCCTACACAGTGTTGAAGTAGAAGTCAGTTACTTCGCAGCTGGAATTATCGC
  CCATTTAATATCCAGAGGTGAACAAGCATGGACCTTGAGCCGAAGCCAGAGGAATTCTCTTCTTGATGAT
  TTGCATTCAGCTATTTTGAAATGGCCAACTCCAGAGTGTGAGATGGTAGCATACAGGTCCTTTAATCCAT
  TCTTTCCACTACTTGGCTGTTTTACGACTCCTGGAGTCCAGCTGTGGGCAGTTTGGGCCATGCAGCACGT
  CTGCAGCAAGAATCCTTCAAGGTATTGCAGCATGCTGATTGAAGAAGGAGGACTGCAGCATTTGTATAAC
  ATCAAAGAACACGAGCAGACAGATCCCTATGTCCAGCAGATTGCTGTGGCCATTCTGGACAGTTTAGAGA
  AACACATTGTGCGCCATGGAAGGCCCCCTCCCTGTAAAAAGCAGCCCCAGGCCCGGCTGAATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033634
• Insert Size: 2235 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033634.3, NP_001028806.2
• RefSeq Size: 10654 bp
• RefSeq ORF: 2235 bp
• Locus ID: 414872
• UniProt ID: Q3UFS0
• Cytogenetics: 4 C7
• Gene Summary: Probably acts as target recruitment subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC.[UniProtKB/Swiss-Prot Function]