Fchsd2 (NM_001146010) Mouse Untagged Clone

CAT#: MC221252

Fchsd2 (untagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fchsd2
• Synonyms: BC034086; mKIAA0769; R74866; Sh3md3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221252 representing NM_001146010
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCGCCGCCGAGGAAGGTGAAAGTTACACAAGAGCTGAGAAACATTCAAGGTGAGCAGATGACAA
  AACTTCAAGCCAAACATCAGGCAGAATGTGACTTACTTGAAGACATGAGGACTTTCAGTCAGAAGAAGGC
  TGCTATTGAAAGAGAGTATGCACAGGGTATCCAGAAGTTGGCTAGTCAATACCTGAAGAGAGATTGGCCT
  GGAATAAAAACTGATGATCGGAATGATTACAGGAGTATGTATCCTGTTTGGAAATCTTTTCTCGAGGGAA
  CAATGCAGGTAGCCCAGTCTCGGATCAATATATGTGAAAACTATAAAAACTTCATTTCTGAACCTGCAAG
  AGCAGTAAGAAGCTTAAAAGAACAGCAACTAAAACGGTGTGTGGATCAGTTGACAAAGATCCAAACAGAA
  TTACAGGAAACTGTGAAAGATTTAGTGAAAGGCAAAAAGAAGTACTTTGAGACTGAACAGATGGCTCATG
  CAGTAAGAGAGAAAGCTGACATCGAAGCAAAGTCTAAACTTAGTCTTTTTCAATCAAGAATCAGTTTACA
  GAAAGCAAGTGTGAAGTTAAAAGCCCGGCGATCTGAATGTAATACCAAAGCTACCCATGCAAGGAATGAT
  TACCTTCTGACGCTAGCAGCAGCTAACGCACATCAGGACCGCTACTATCAAACAGACTTAGTTAACATCA
  TGAAGGCTCTTGATGGAAACGTGTATGACCACCTCAAGGATTATTTAATAGCCTTCAGCCGGACCGAGCT
  AGAAACTTGCCAAGCTATACAGAATACATTCCAGTTTTTACTAGAAAACTCCAGCAAGGTGGTACGGGAC
  TATAACCTTCAGCTGTTTCTACAAGAGAATGCTGTATTTCACAAGCCTCAGCCCTTCCAGTTCCAGCCTT
  GTGACAGTGATACTAGTCGACAATTAGAATCAGAAACTGGTACCACAGAGGAACACAGTCTAAATAAGGA
  GGCTCGGAAATGGGCCACACGTGTGGCACGTGAGCACAAAAATATTGTTCATCAGCAGCGGGTTCTAAAT
  GAACTAGAATGTCATGGAGTTGCTCTATCAGAACAGAGCCGAGCAGAGCTGGAACAGAAAATAGATGAAG
  CAAGAGAAAGTATCCGTAAAGCAGAGATCATTAAATTGAAAGCTGAAGCCCGGCTAGACCTACTGAAGCA
  GATTGGGGTTTCTGTAGATACGTGGTTAAAGAGTGCCATGAACCAAGTGATGGAGGAACTAGAAAATGAG
  CGGTGGGCCCGTCCTCCAGCCGTGACCAGCAATGGCACTCTACACTCGCTTAATGCAGATGCTGAAAGAG
  AAGAAGGAGAAGAGTTTGAAGACAACATGGATGTTTTTGATGACAGCAGCTCCAGCCCTTCTGGCACCTT
  GAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTATAAGGCTTCTCAACCAGATGAGTTGACCATT
  GAGGAACATGAGGTGTTAGAAGTGATTGAAGATGGAGATATGGAAGACTGGGTAAAGGCTCGAAATAAAG
  TTGGCCAAGTGGGTTATGTGCCAGAAAAGTACCTACAATTTCCCACCTCGAACAGCCTACTGAGCATGCT
  ACAGTCTTTGGCTGCTTTGGACAGTCGGTCACACACATCCAGCAATTCCACAGAAGCAGAACTCGTTTCA
  GGCAGCCTGAATGGAGATGCCAGTGTCTGTTTTGTGAAAGCACTTTATGATTATGAGGGCCAGACAGATG
  ATGAGCTGTCCTTTCCCGAGGGAGCAATTATTCGCATCTTGAACAAAGAAAACCAAGATGATGATGGCTT
  CTGGGAAGGGGAGTTCAGTGGTCGAATCGGTGTTTTCCCATCGGTGCTAGTGGAAGAGCTCTCAGCCTCC
  GAGAATGGCGACACTCCATGGACAAGAGAGATCCAGATCTCTCCATCCCCCAAGCCTCACACATCCCTGC
  CTCCACTGCCTCTGTATGACCAGCCACCCAGCAGCCCGTATCCCAGTCCAGATAAGAGGAGCTCCCAGTT
  CTTCCCCCGGTCTCCTTCAGCCAATGAAAACAGCCTTCATGCTGAATCACCAGGATTCTCACAGGCCTCA
  AGACAGACTCCTGACACCTCATATGGCAAGCTGCGGCCTGTTCGGGCGGCGCCGCCACCACCCACACAGA
  ACCACCGGCGGACAACTGAGAAGATGGAGGACGTGGAGATCACACTGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146010
• Insert Size: 2223 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146010.1, NP_001139482.1
• RefSeq Size: 4360 bp
• RefSeq ORF: 2223 bp
• Locus ID: 207278
• UniProt ID: Q3USJ8
• Cytogenetics: 7 E2
• Gene Summary: Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor. Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling. Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits. Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (By similarity). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex (PubMed:23437151). Does not promote actin polymerisation in the absence of membranes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the mid coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.