Rnasel (NM_011882) Mouse Untagged Clone

CAT#: MC221163

Rnasel (untagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnasel
• Synonyms: E230029I04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221163 representing NM_011882
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCCCGGATTATAACACACCTCAGGGTGGAACCCCATCAGCGGGAAGTCAGAGGACCGTTGTCG
  AAGATGATTCTTCGTTGATCAAAGCTGTTCAGAAGGGAGATGTTGTCAGGGTCCAGCAATTGTTAGAAAA
  AGGGGCTGATGCCAATGCCTGTGAAGACACCTGGGGCTGGACACCTTTGCACAACGCAGTGCAAGCTGGC
  AGGGTAGACATTGTGAACCTCCTGCTTAGTCATGGTGCTGACCCTCATCGGAGGAAGAAGAATGGGGCCA
  CCCCCTTCATCATTGCTGGGATCCAGGGAGATGTGAAACTGCTCGAGATTCTCCTCTCTTGTGGTGCAGA
  CGTCAATGAGTGTGACGAGAACGGATTCACGGCTTTCATGGAAGCTGCTGAGCGTGGTAACGCTGAAGCC
  TTAAGATTCCTTTTTGCTAAGGGAGCCAATGTGAATTTGCGACGACAGACAACGAAGGACAAAAGGCGAT
  TGAAGCAAGGAGGCGCCACAGCTCTCATGAGCGCTGCTGAGAAGGGCCACCTGGAAGTCCTGAGAATTCT
  CCTCAATGACATGAAGGCAGAAGTCGATGCTCGGGACAACATGGGCAGAAATGCCCTGATCCGTACTCTG
  CTGAACTGGGATTGTGAAAATGTGGAGGAGATTACTTCAATCCTGATTCAGCACGGGGCTGATGTTAACG
  TGAGAGGAGAAAGAGGGAAAACACCCCTCATCGCAGCAGTGGAGAGGAAGCACACAGGCTTGGTGCAGAT
  GCTCCTGAGTCGGGAAGGCATAAACATAGATGCCAGGGATAACGAGGGCAAGACAGCTCTGCTAATTGCT
  GTTGATAAACAACTGAAGGAAATTGTCCAGTTGCTTCTTGAAAAGGGAGCTGATAAGTGTGACGATCTTG
  TTTGGATAGCCAGGAGGAATCATGACTATCACCTTGTAAAGCTTCTCCTCCCTTATGTAGCTAATCCTGA
  CACCGACCCTCCTGCTGGAGACTGGTCGCCTCACAGTTCACGTTGGGGGACAGCCTTGAAAAGCCTCCAC
  AGTATGACTCGACCCATGATTGGCAAACTCAAGATCTTCATTCATGATGACTATAAAATTGCTGGCACTT
  CCGAAGGGGCTGTCTACCTAGGGATCTATGACAATCGAGAAGTGGCTGTGAAGGTCTTCCGTGAGAATAG
  CCCACGTGGATGTAAGGAAGTCTCTTGTCTGCGGGACTGCGGTGACCACAGTAACTTAGTGGCTTTCTAT
  GGAAGAGAGGACGATAAGGGCTGTTTATATGTGTGTGTGTCCCTGTGTGAGTGGACACTGGAAGAGTTCC
  TGAGGTTGCCCAGAGAGGAACCTGTGGAGAACGGGGAAGATAAGTTTGCCCACAGCATCCTATTATCTAT
  ATTTGAGGGTGTTCAAAAACTACACTTGCATGGATATTCCCATCAGGACCTGCAACCACAAAACATCTTA
  ATAGATTCCAAGAAAGCTGTCCGGCTGGCAGATTTTGATCAGAGCATCCGATGGATGGGAGAGTCACAGA
  TGGTCAGGAGAGACTTGGAGGATCTTGGACGGCTGGTTCTCTACGTGGTAATGAAAGGTGAGATCCCCTT
  TGAGACACTAAAGACTCAGAATGATGAAGTGCTGCTTACAATGTCTCCAGATGAGGAGACTAAGGACCTC
  ATTCATTGCCTGTTTTCTCCTGGAGAAAATGTCAAGAACTGCCTGGTAGACCTGCTTGGCCATCCTTTCT
  TTTGGACTTGGGAGAACCGCTATAGAACACTCCGGAATGTGGGAAATGAATCTGACATCAAAGTACGGAA
  ATGTAAAAGTGATCTTCTCAGACTACTGCAGCATCAGACACTTGAGCCTCCCAGAAGCTTTGACCAGTGG
  ACATCTAAGATCGACAAAAATGTTATGGATGAAATGAATCATTTCTACGAAAAGAGAAAAAAAAACCCTT
  ATCAGGATACTGTAGGTGATCTGCTGAAGTTTATTCGGAATATAGGCGAACACATCAATGAGGAAAAAAA
  GCGGGGAATGAAGGAGATACTTGGAGATCCTTCTCGTTATTTTCAGGAGACATTTCCAGATCTTGTAATC
  TATATCTACAAGAAACTAAAGGAAACAGAATACAGAAAACACTTCCCACAGCCTCCACCACGCCTCAGCG
  TTCCAGAGGCAGTGGGACCAGGGGGCATACAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011882
• Insert Size: 2208 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011882.2, NP_036012.1
• RefSeq Size: 4758 bp
• RefSeq ORF: 2208 bp
• Locus ID: 24014
• UniProt ID: Q05921
• Cytogenetics: 1 G3
• Gene Summary: Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover (By similarity). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length (By similarity).[UniProtKB/Swiss-Prot Function]