Lrrfip1 (NM_001111311) Mouse Untagged Clone

CAT#: MC221155

Lrrfip1 (untagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrrfip1
• Synonyms: AU024550; Fliiap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221155 representing NM_001111311
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGCCCAGAGGGCGCGCAAAACAAGGAAATCGACTGTCTGAGTCCAGAGGCGCAAAGACTGGCGG
  AGGCCAGGCTCGCAGCGAAGCGTGCAGCCCGAGCCGAGGCTCGAGAGATCCGGATGAAGGAGCTGGAACG
  GCAGCAGAAAGAGGTAGAAGAAAGACCAGACAAAGACTTTGCTGAGAAGGGCTCCCGTAACATGCCTAGC
  CTGTCTGCAGCCACACTGGCATCTCTGGGTGGGACTTCCTCCCGGAGAGGGAGTGGAGATACCTCCATCT
  CCATGGACACCGAAGCATCCATCAGGGAAATCAAGGACTCGCTGGCCGAAGTTGAGGAGAAGTACAAGAA
  AGCTATGGTCTCCAACGCCCAGCTAGACAATGAGAAGACCAACTTCATGTACCAGGTGGACACACTGAAG
  GACATGCTGCTGGAGCTGGAAGAGCAGCTGGCCGAGTCTCAGCGGCAATACGAGGAGAAGAACAAGGAGT
  TCGAGAGGGAGAAGCATGCCCACAGCATCCTGCAGTTCCAGTTTGCCGAAGTGAAAGAGGCCCTGCGGCA
  AAGGGAAGAGATGCTCGAGAAACATGGAATAATCCTAAATTCAGAAATAGCCACCAACGGAGAGACTTCA
  GACACAGTAAATGACGTTGGGTATCAAGCCCCTACGAAGATAACAAAAGAAGAGCTGAATGCCCTCAAGT
  CGGCCGGGGAGGGGACCCTAGGAAAAGCCAAAGAAGTGGAGGTGAAAAAGGAAATTGTGGAGAAAGTGGG
  GCAAAGAGAAACCTTACAGAATTCTGAGCAAGAACAGCCCAAACCCAACACAGGAAAGGATTGTGTGGAC
  AGAGGGGTGTCACATCCTGGTGAGAAGGCTGAGAACCAGAGACCCGCTGAAGACAGTGCCCTGTCCCCGG
  GACCACTAGCAGGGGCCAAATGTGAGCAACAGGTTCAGAGCCAAGATCAAGAGAATACTTCTGACCTCAA
  AAACTCAGAGCAGATCGAGTCACACAAGGTCACAAACAAGTCAGATAGTAGGGCCAGTAACTCCCCTGAA
  CAGTCCAGCTGCCTGGAAGGTCTAGACAGTGAAGTGCCAGGGCCCACTGAGGATTTGAAAACAGATTTGG
  GGAAAGGTAGCTTTGAACCATGTCCAGACTACATTTTGGGGCAAACAGCTGAAATTGACAAGGTGACCTG
  TACAGACTCAAGAGGCACAGGTGGAAACCAAAGGGAGGATGAGGTACAGGCAGGGGACACCACAGTTGAG
  GACCAAGTAGGGACAGTGGCTTCTGGACCTGCAAAGCAAAGCAAAGGCACCGAGAATCATGGTGAAAGTT
  GTCTGAAGGATGGGCTGGGACAGAGCTCAGAGAGAGAACTCACCCAAGAAGTAGCTGAGCCTGAGGAAGC
  CATCGTTCAGATCCCACAGGCCGGTGGAGAGAACACTATTACCAAGGCAGATGATGCCGAAGGAAGGGAT
  GAGAAGCCCATCCAGGCAGAAGCCCAGGCAAGCCCTGGAGCTCCCATAAATCAGAGCGGCCATCAGGACA
  CAACAGGACCAGGCAGCACCGATGCCCAGCGCACACCACCTCATGCAAAAGAGCGCAAGAAACAAGGGAA
  GAGCGAGCAGCAGGCAGAGGCCTTGGATTCACCGCAGAAGAAGACGAAGAACAAGAAGAAGAAGAACAAG
  AAGAAGAAAGCTGCAACTCCCGCGGAAACCTGCAGAGATGCCAATGAAGAGTTGAACTGTCAGGACCCAG
  ATGTGGGTGACATGGAGGAGGAAGAGCGGCTCCAGGTCACTGACAAGAAACAGGCATCAGGAAGCCCAGA
  GCAGAAAATCAGAGCGGGAAGCAGAGAACCTGTCGAAGATCCTCAAAGTGGGTCGAGTGGAAAACAGAAC
  AAAGTGGAAGAGGATGGTCCCACGGAAGGTCCCACAGACATCTTGGATCAGAACAGTCCCCAGTGTGAAG
  ACAGGGAGATCTCCCCAGTGGGAGAGAAAGGTCCCCAATGCGATACCTCTCAGATAGGTAGTGAAGAGGG
  ACATGTGACCTCCCAACACGGTGGTCAGGCAGTTGAGAACCACAACCTAGATAACAGTGACCTGTCAGGG
  CAGCTGGAGGGCTTCAATTCAGAAAGTGGAGGGCAGGCAAGAGAGGAGGTTGGGAATAGCAAGAGTAAGG
  AGGACTGCACCATGTCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001111311
• Insert Size: 2190 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111311.1, NP_001104781.1
• RefSeq Size: 3662 bp
• RefSeq ORF: 2190 bp
• Locus ID: 16978
• UniProt ID: Q3UZ39
• Cytogenetics: 1 D
• Gene Summary: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA (By similarity). Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1).