Hic1 (NM_001098203) Mouse Untagged Clone

CAT#: MC221154

Hic1 (untagged) - Mouse hypermethylated in cancer 1 (Hic1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hic1
• Synonyms: AA408311; HIC-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221154 representing NM_001098203
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTTCCTGAAGCGGACATTTTACTTAAATCGGGAGAGTGTGCTGGGCAGACGATGCTGGACACGA
  TGGAGGCGCCTGGCCATTCGAGGCAGCTACTGCTGCAGCTCAACAATCAGCGCACCAAGGGCTTCTTGTG
  CGACGTGATCATCGTGGTGCAGAATGCCCTCTTCCGCGCGCACAAGAACGTGCTGGCGGCCAGCAGCGCC
  TACCTCAAGTCCCTGGTGGTGCATGACAACCTGCTAAACCTGGACCATGACATGGTGAGCCCGGCCGTGT
  TCCGCCTGGTGCTGGACTTCATCTACACCGGCCGCCTGACTGACAGTGTTGAGGCCGCAGCAGCAGCAGC
  GGTGGCCCCGGGCGCGGAGCCGAGCCTGGGCGCTGTGCTGGCTGCTGCCAGCTACCTGCAGATCCCTGAC
  CTCGTGGCTCTGTGCAAGAAGCGCCTCAAACGCCACGGCAAGTACTGCCACCTGCGGGGAGGAGGCAGCG
  GCGGCGGCGGCTACGCTCCTTACGGGCGGCCCGGCCGGGGCTTGAGGGCTGCCACGCCCGTCATCCAGGC
  TTGCTACTCGTCCCCGGCCGGGCCACCGCCGCCGCCTGCCGCCGAGCCGCCGTCGGGCCCCGATGCAGCC
  GTCAACACCCACTGCGCTGAGCTATATGCTTCAGGCCCGGGCCCAGCAGCCTCACTCTGCGCCCCGGAGC
  GTCGCTGCTCCCCGCTTTGCGGCCTGGATCTGTCCAAGAAGAGCCCGCCAGGTTCCTCGGTCCCCGAGCG
  ACCGCTTAGTGAGCGCGAACTGCCTCCACGCCCGGATAGCCCTCCCGGTGCGGGGCCCGCAGTCTACAAG
  GAGCCATCACTCGCCCTGCCCCCGCTGCCGCCTTTGCCCTTCCAAAAGCTGGAGGAGGCCGTACCGACTC
  CAGACCCGTTTCGAGGAAGCGGTGGCAGTCCGGGACCCGAGCCCCCCGGCCGCCCCGACGGCTCCAGCCT
  CCTCTACCGCTGGATGAAGCACGAGCCAGGCCTGGGTAGTTATGGCGATGAACTGGTCCGGGATCGAGGC
  TCCCCGGGTGAGCGCCTGGAGGAGCGCGGTGGGGATCCTGCCGCCTCACCCGGGGGCCCCCCGCTGGGCC
  TGGTACCCCCGCCACGCTACCCCGGGAGCCTGGACGGGCCAGGCACAGGAGCAGACGGCGACGACTATAA
  GAGCAGCAGCGAGGAGACCGGTAGCAGCGAAGACCCCAGCCCACCCGGCGGCCACCTGGAGGGCTACCCA
  TGCCCGCACTTGGCTTATGGTGAGCCTGAGAGCTTTGGTGACAACCTGTACGTGTGCATCCCATGTGGCA
  AAGGCTTCCCCAGCTCGGAACAGCTGAATGCACACGTGGAGGCTCACGTAGAGGAAGAAGAGGCGTTATA
  TGGCAGGGCAGAGGCTGCTGAGGTGGCTGCTGGGGCCGCCGGCCTTGGGCCCCCCTTTGGTGGCGGTGGG
  GACAAGGTCACTGGGGCGCCGGGCGGACTAGGAGAGCTGCTGCGGCCATACCGCTGCGCGTCCTGCGACA
  AGAGCTACAAGGACCCGGCCACGCTGAGGCAGCACGAGAAGACACACTGGCTGACAAGGCCCTATCCGTG
  TACCATCTGCGGAAAGAAGTTCACGCAACGCGGAACCATGACACGCCATATGCGCAGCCACTTGGGCCTG
  AAGCCCTTTGCGTGCGACGCGTGCGGCATGCGCTTCACCCGCCAGTATCGCCTCACGGAGCACATGCGCA
  TCCACTCTGGAGAGAAGCCCTACGAGTGCCAGGTGTGCGGTGGCAAGTTTGCTCAACAGCGCAACCTCAT
  CAGCCATATGAAGATGCACGCTGTAGGTGGCGCGGCCGGCGCGGCCGGGGCGCTGGCTGGCCTGGGGGGA
  CTACCTGGCGTCCCTGGCCCTGACGGCAAGGGCAAGCTCGACTTCCCTGAGGGTGTCTTTGCTGTGGCCC
  GCCTCACAGCTGAACAGCTGAGTCTGAAGCAGCAGGACAAGGCAGCTGCTGCCGAGCTGCTGGCGCAGAC
  CACGCACTTCCTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACCCGCTGGCTAAATTCACTGCTGAG
  CTAGGACTCAGCCCAGACAAGGCGGCAGAGGTGCTGAGCCAGGGTGCGCACTTGGCCGCAGGACCGGACA
  GCCGAACCATCGACCGTTTCTCTCCCACCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001098203
• Insert Size: 2202 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098203.1, NP_001091673.1
• RefSeq Size: 3253 bp
• RefSeq ORF: 2202 bp
• Locus ID: 15248
• UniProt ID: Q9R1Y5
• Cytogenetics: 11 45.76 cM
• Gene Summary: Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from ACKR3, FGFBP1 and EFNA1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 5' terminal exon including the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.