Mapk6 (NM_015806) Mouse Untagged Clone

CAT#: MC221096

Mapk6 (untagged) - Mouse mitogen-activated protein kinase 6 (Mapk6), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk6
• Synonyms: 2610021I23Rik; D130053K17Rik; Erk3; Mapk4; Mapk63; Prkm4; Prkm6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221096 representing NM_015806
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGAAATTCGAAAGTCTCATGAACATTCATGGCTTTGATCTGGGCTCTAGGTACATGGACTTAA
  AACCATTGGGCTGCGGAGGCAATGGCTTGGTCTTTTCTGCTGTAGACAATGACTGTGACAAAAGAGTAGC
  CATCAAGAAAATTGTCCTCACCGATCCCCAGAGTGTCAAACATGCTCTCCGTGAAATCAAAATTATTCGA
  AGACTTGACCACGATAACATTGTGAAAGTGTTTGAAATTCTTGGTCCCAGTGGAAGCCAGTTAACAGACG
  ATGTGGGCTCTCTCACGGAGCTGAATAGCGTCTACATTGTTCAGGAGTATATGGAGACAGACTTGGCGAA
  TGTGCTGGAGCAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGTGGGCTC
  AAATATATCCATTCTGCAAACGTACTGCACAGAGATCTCAAGCCAGCTAATCTTTTCATTAACACTGAAG
  ACTTGGTGCTGAAGATAGGTGACTTTGGCCTGGCACGGATCATGGATCCTCATTATTCCCATAAGGGTCA
  TCTTTCTGAAGGATTGGTTACCAAATGGTACAGATCTCCACGGCTTTTACTTTCTCCTAATAATTACACT
  AAAGCCATTGACATGTGGGCTGCAGGCTGCATCTTTGCTGAGATGCTGACTGGTAAAACCCTCTTTGCAG
  GTGCACATGAACTTGAACAGATGCAGCTGATCCTCGACTCCATCCCTGTTGTGCACGAGGAGGATCGGCA
  GGAGCTTCTCAGCGTGATTCCAGTTTACATTAGAAACGACATGACTGAGCCACACAGACCGCTCACTCAG
  CTGCTGCCGGGGATCAGCCGAGAAGCACTGGATTTCCTGGAGCAGATTCTGACTTTCAGCCCCATGGACC
  GGCTGACAGCTGAGGAAGCCCTTTCCCATCCTTACATGAGCATCTACTCCTTCCCGACGGATGAGCCCAT
  CTCAAGCCACCCTTTCCACATAGAGGACGAAGTGGACGACATTTTGCTTATGGATGAAACACACAGTCAC
  ATTTATAACTGGGAAAGGTACCACGATTGTCAGTTCTCGGAGCATGACTGGCCTATTCATAACAACTTTG
  ATATCGATGAGGTGCAGCTTGACCCAAGAGCTCTGTCTGACGTCACCGATGAAGAAGAAGTCCAAGTTGA
  TCCTCGAAAATACTTGGATGGAGACCGAGAGAAGTATCTAGAGGATCCCGCCTTCGACACCAGCTACTCT
  GCTGAACCCTGCTGGCAGTACCCAGATCACCACGAGAACAAGTACTGCGATCTGGAGTGTAGCCACACCT
  GTAACTACAAAACAAGATCATCACCATACTTAGATAACCTGGTGTGGAGGGAGAGCGAGGTTAACCATTA
  CTATGAGCCCAAGCTTATTATAGATCTTTCCAACTGGAAAGAGCAAAGTAAAGAAAAATCCGATAAGAGA
  GGCAAGTCCAAGTGTGAGAGGAACGGGTTGGTCAAGGCGCAGATCGCGCTAGAGGAAGCATCCCAGCAGC
  TGGCTGAGAGGGAGAGGGGCCAAGGCTTCGACTTTGACTCCTTCATCGCGGGGACCATTCAGCTCAGTGC
  CCAGCATCAGTCTGCTGACGTAGTTGACAAGTTAAACGACTTGAATAGCTCAGTGTCCCAGCTAGAATTG
  AAAAGCCTGATATCCAAGTCAGTCAGCCGAGAAAAGCAAGAAAAGGGAAGGGCTAACCTGGCCCAGCTGG
  GAGCCTTGTACCAGTCCTCCTGGGACAGCCAGTTTGTGAGTGGCGGGGAGGAGTGCTTCCTCATCAGTCA
  GTTTTGTTGTGAGGTCAGGAAGGACGAGCATGCGGAGAAGGAGAACACCTACACCAGCTATTTGGACAAG
  TTTTTTAGCAGGAAGGAGGATTCCGAAATGCTAGAAACTGAGCCAGTGGAGGAAGGGAAGCGTGGGGAGA
  GAGGCCGTGAGGCAGGGCTTCTGAGCGGCGGTGGGGAGTTCCTCCTGAGCAAGCAGCTGGAGTCCATAGG
  CACCCCGCAGTTCCACAGCCCAGTGGGGTCCCCACTCAAGTCCATCCAGGCCACCTTGACACCTTCCGCT
  ATGAAATCTTCCCCTCAAATCCCTCACAAGACATACAGCAGCATTCTGAAACATCTGAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015806
• Insert Size: 2163 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015806.5, NP_056621.4
• RefSeq Size: 4205 bp
• RefSeq ORF: 2163 bp
• Locus ID: 50772
• UniProt ID: Q61532
• Cytogenetics: 9 42.3 cM
• Gene Summary: Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same protein.