Uimc1 (NM_011307) Mouse Untagged Clone

CAT#: MC221087

Uimc1 (untagged) - Mouse ubiquitin interaction motif containing 1 (Uimc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Uimc1
• Synonyms: 9430016E08Rik; D330018D10Rik; D630032M02Rik; RIP110; Rxrip110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221087 representing NM_011307
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACGAAGGAAGAAAAAAATTAAAGAGGCCTCTGAAAGCCAGAACTTGGAGAAGAAGGATCTGGAAA
  CAAGCAGTTGTGTCAGTATAAAGAAGAAGCGTAGACTTGAGGACTTACTCATAGTGATATCCGATAGCGA
  TGGAGAGGAAACAAAAGAGGAGAATGGATTGCAGAAAACGAAGACAAAACAGTCGAACAGATCAAAGTGT
  TTGGCTAAAAGAAAAGTTGCACACATGTCAGAAGAAGAACAATTTGCTTTGGCTCTCAAGATGAGTGAGC
  AGGAAGCTAGGGAGGTGAATAACCAGGAGGAGAAAGAAGAGGAGCTCTTGCGGAAAGCCATTGCTGAAAG
  CCTGAATAGTTGCTGGTCTTCTGCTGCTTCTGCTACCAGATCTCGACCTCTGGCTGCTGAACTATCTTCA
  CATTCCCATCAAGAGAACACCAAAGACTCTGGGACCACTGAAGGCGTATGGCAGCTGGTACCTCCATCAC
  TGTGTAAAGGCTCACATGTCAGTCAGGGAAACGAGGCTGAGCAAAGAAAGGAGCCCTGGGACCACAATGA
  AAACACTGAAGAGGAGCCGGTCTCTGGCAGCTCAGGAAGCTGGGACCAGTCAAGCCAGCCAGTGTTTGAG
  AATGAGAACGTTAAATGTTTTGACAGATGTACTGGCCACTTGGCTGAGCACACACAGTGTGGGAAGCCAC
  AGGAAAGTACTGGGAGTGGTTATGCTTTTTCCAAAGCTGTCCAGGGTAGGGGGGACACGTCTAGGCAATG
  CCTTCCTATCCCAGCAGACACAAAAGGTCTCCAGGACACTGGGGGCACTGTGCACTACTACTGGGGTATT
  CCATTCTGCCCTGCTGGAGTAGATCCCAATCAATACACCAATGTCATTCTCTGCCAGTTAGAGGTTTATC
  AGAAGAGCCTGAAAATGGCTCAGAGACAGCTTGTTAAAAAAAGAGGGTTTGGGGAACCAGTGTTACCTAG
  ACCTCCTTTTCTGATCCAGAATGAATGTGGCCAAGAAGATCAGACTAGTGACAAAAATGAAGGCATCTCA
  GAAGATATGGGAGATGAAGCCAAAGAGGAAAGGCAGGAATCTAGGGCATCTGTCTGGCACTCAGAAACCA
  AGGATTTTCAAAAAAGTCCAATTAAAAGCTTGAAACAGAAACTTTTGTTGGAGGAAGAACCAACAACCAG
  TCGTGGTCAGTCTTCCCAAGGTCTGTTTGTTGAAGAAACCTCTGAAGAAGGTCTGAAGAGTTCTGAAGGA
  GACAACTCTGTGCCCACCACGCAAAGCATTGCAGCTTTGACCAGTAAGAGAAGTTTAGTTCTTATGCCGG
  AAAGTTCTGCAGAAGAAACCACTGTTTGCCCTGAGACACAGTTAAGTTTCCTTGAACCCCTTGACCTCAA
  TAGAGAAGACTCTCCAGATAGCAGAGAGCTCCCCATTGAAGTAAGGATGGCAGTGGGCGATAAGCAGGTT
  GCTAATAGGGAAGATTGTATGAAGGAAAACCCTCCTCCTGCAGTCTCATCTAGTACCCGGGTATCCTGCC
  CACTGTGTAACCAAGACTTTCCTCCCACAAAGATTGAACAGCATGCCATGTACTGCAATGGTCTGATGGA
  GCAGGAAACAGTGTTGACTCGGAGACGAAGAGAGGCCAAGAACAAGAGTGACGGTCGGACAGCTGCACAG
  CCGGCTCTGGATGCCAACAGGAAGGAGAAGTGTTATCTATGTAAGTCCCTGGTTCCACTTGGGGAGTATC
  AGTGCCATGTGGAGGCCTGTCTCCAGCTTGCAAAGGTTGACAGAGAAGATGGGATTGAAGGGACAAGGAG
  ACCAAGGGTGTGTGCACCTGTGGAGGGGAAACAACAGCAGCGGCTGAAGAAGTCAAAGGACAAAGGCCAT
  AGTCAAGGCCGACTCCTCAGTCTCTTGGAGCAGTCTGAGCATAGGACCACAGGTGTAGAGAAAAAACCCA
  AGTATTCGGAAGTAAGAACCTTCAGGATGCCCTCACCAGAGGTGGAAGAGGCTAGCTGCAGCAGAGAGAT
  GCAGAGTACCCTCTCACAGCTCAACTTAAATGAGTCTCCCATCAAGTCTTTTGTTCCTGTTTCAGAAGCT
  ACAAATTGCTTAGTGGACTTTAAAGAACAGTTTGCTTTCCGGTCACGAACTAAATCAGGCAGAGGAAGGA
  GGAGAAAATCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011307
• Insert Size: 2184 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011307.2, NP_035437.1
• RefSeq Size: 2931 bp
• RefSeq ORF: 2184 bp
• Locus ID: 20184
• UniProt ID: Q5U5Q9
• Cytogenetics: 13 B1
• Gene Summary: Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19536136). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).