Trpv6 (NM_022413) Mouse Untagged Clone

CAT#: MC221065

Trpv6 (untagged) - Mouse transient receptor potential cation channel, subfamily V, member 6 (Trpv6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpv6
• Synonyms: Cac; CAT; CaT1; Ecac2; Otrpc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221065 representing NM_022413
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ACGGGACCTCTACAGAGAGAGGACAGGCCGGCTCTTGGGGGTGCCAATGTGGCCCCAGGGTCGAGCCCAG
  TTGGGGTCTGGCATCAGCCTCAGCCCCCCAAGGAACCAGCCTTCCACCCCATGGGGTGGTCCCTGCCCAA
  GGAGAAGGGGTTAATACTCTGCCTATGGAACAAGTTCTGCAGATGGTTCCACAGACAAGAGTCCTGGGCT
  CAGAGCCGAGACGAGCAGAACCTGCTGCAGCAGAAGAGGATCTGGGAATCACCTCTTCTTCTAGCTGCCA
  AAGAAAACGATGTCCAGGCTCTGAGCAAGCTGCTCAAGTTTGAAGGATGTGAGGTGCACCAGAGAGGAGC
  CATGGGGGAAACTGCACTTCACATAGCAGCCCTCTATGATAACCTGGAGGCTGCAATGGTGCTAATGGAG
  GCTGCCCCAGAACTAGTCTTTGAGCCCATGACTTCGGAGCTATATGAAGGTCAGACTGCACTACACATTG
  CAGTAATAAACCAGAATGTAAACTTGGTCCGTGCTCTGCTTGCCCGAGGGGCCAGTGTCTCTGCCAGAGC
  TACAGGCTCTGTCTTCCACTACAGGCCTCACAACCTCATTTACTATGGAGAGCACCCTTTGTCCTTTGCT
  GCCTGTGTGGGTAGTGAGGAGATTGTTAGACTGCTCATTGAGCATGGAGCTGACATCCGTGCCCAGGACT
  CTCTGGGAAATACAGTACTACACATACTCATCCTGCAGCCCAACAAAACCTTTGCCTGCCAGATGTACAA
  CTTGCTACTGTCCTATGATGGGGGAGACCACCTGAAGTCCCTGGAACTTGTGCCAAATAACCAGGGACTC
  ACCCCCTTCAAGTTGGCTGGGGTGGAAGGCAACATTGTGATGTTCCAACACCTGATGCAGAAGCGGAAAC
  ACATCCAGTGGACATATGGGCCATTGACCTCCACACTGTATGACCTCACTGAGATTGACTCCTCAGGAGA
  TGATCAATCCCTACTGGAACTTATTGTTACTACCAAGAAACGGGAGGCTCGCCAGATCCTGGACCAGACA
  CCTGTAAAGGAACTGGTGAGCCTCAAGTGGAAGAGGTATGGGCGGCCCTACTTCTGCGTGCTGGGTGCCA
  TCTATGTGCTGTACATCATCTGCTTTACCATGTGCTGTGTCTACCGCCCGCTCAAGCCCAGGATCACTAA
  CCGCACCAACCCCCGGGACAACACTCTGATGCAACAGAAGCTCCTTCAGGAGGCCTATGTGACCCCTAAG
  GATGACCTCCGGTTGGTGGGGGAACTCGTGAGCATCGTTGGGGCTGTGATCATCCTGCTGGTGGAGATTC
  CAGACATCTTCAGGTTGGGGGTCACTCGATTCTTTGGACAGACCATTCTCGGGGGGCCATTCCATGTCAT
  CATTATCACTTATGCCTTCATGGTGCTGGTGACCATGGTGATGCGACTCACCAATGTAGATGGAGAAGTG
  GTGCCCATGTCCTTTGCTCTGGTGTTGGGCTGGTGCAATGTCATGTACTTTGCCAGAGGATTCCAGATGC
  TGGGTCCCTTCACCATCATGATTCAGAAGATGATTTTTGGTGACTTGATGCGATTCTGCTGGCTGATGGC
  TGTGGTAATTCTGGGATTTGCTTCAGCCTTCTATATCATTTTCCAGACAGAGGATCCCGATGAGCTGGGT
  CATTTCTATGACTACCCCATGGCACTGTTCAGCACCTTTGAACTCTTCCTCACCATCATCGATGGCCCTG
  CCAACTATGACGTGGATCTGCCCTTCATGTACAGCGTTACCTACGCTGCCTTTGCCATCATCGCCACACT
  GCTCATGCTTAACCTCCTCATTGCCATGATGGGTGACACTCACTGGAGAGTTGCCCATGAGCGAGATGAG
  CTCTGGAGAGCACAGGTTGTGGCCACCACTGTGATGTTAGAGCGGAAGCTACCTCGTTGCCTGTGGCCTC
  GCTCTGGGATATGTGGGCGAGAGTATGGTCTTGGCGACCGCTGGTTCTTGAGGGTGGAAGACAGGCAAGA
  TCTCAACAGACAACGCATCCGCCGCTATGCACAGGCCTTCCAGCAACAAGATGGCCTCTACTCTGAGGAC
  TTGGAAAAAGATTCAGGAGAAAAACTGGAGACAGCACGTCCCTTTGGTGCCTATCTGTCCTTTCCTACAC
  CCTCAGTATCTCGAAGTACCTCCCGAAGCAGCACCAATTGGGAAAGGCTTCGACAAGGGGCCCTGAGGAA
  GGACCTGCGGGGGATAATCAACCGGGGCCTAGAAGATGGGGAGGGCTGGGAGTACCAGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022413
• Insert Size: 2304 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022413.4, NP_071858.3
• RefSeq Size: 2946 bp
• RefSeq ORF: 2304 bp
• Locus ID: 64177
• UniProt ID: Q91WD2
• Cytogenetics: 6 B2.1
• Gene Summary: Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine (PubMed:12765696, PubMed:12601087, PubMed:12574114, PubMed:14736889, PubMed:15123711, PubMed:17129178). Important for normal Ca(2+) ion homeostasis in the body, including bone and skin (PubMed:17129178, PubMed:22878123). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation (PubMed:15123711). In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner (PubMed:12601087). Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (PubMed:12574114).[UniProtKB/Swiss-Prot Function]