St5 (NM_029811) Mouse Untagged Clone

CAT#: MC221022

St5 (untagged) - Mouse suppression of tumorigenicity 5 (St5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: St5
• Synonyms: 2010004M01Rik; 2610305K15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221022 representing NM_029811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGACTGCCAACAAGAACTCCAGCATCACTCATGGAACCGGAGGCACTAAGGCCCCTCGGGAGA
  CGCTGAGCAGAAAATCCTTCGAGTTTGAGGATGCATCCAGTCTCCAATCCCTGTATCCCTCTTCTCCTAC
  TGAGAATGGTACTGAGAGTCAACCCAAGTTTGGATCCAAAAGCACTTTAGAAGAGAATGCCTATGAAGAT
  ATTGTGGGAGGGCTTCCCAAGGAGAATCCCTACGAGGATGTGGACTTAAAGAACCGGAGAGCTGGACGAA
  AATCCCAGCAACTGTCTGAGAACTCCTTGGACTCTTTGCACAGGATGTGGAGTCCCCAAGACAGGAAGTA
  CAACCACCCACCCATGCAGCTGTCCCTGAAGTCCAACAGCCAGTCCCTGCGCAGTGGGAACTGGTCAGAA
  AGGAAGAGCCATCGTTTGCCACGATTACCCAAGAGGCATAGCCATGATGACATGATGCTGTTGGCTCAGC
  TGAGCCTGCCATCTTCACCCTCCAGCCTCAATGAGGACAGCCTCAGCACCACAAGTGAGCTGCTGTCCAG
  CCGCAGGTCCCGCCGCATTCCCAAGCTTGTCCAAAGAATTAACTCTATCTATAATGCCAAGAGAGGAAAG
  AAGAGGTTGAAAAAGCTGTCTATGTCCAGCCTTGAGACAGCATCATTGAGAGATGAGAACAGTGAAAGCG
  AGAGTGACTCTGATGACAGGTTCAAAGCCCACACCCAGCGCTTGGTCCACATCCAGTCGATGCTGAAGCG
  AGCGCCCAGCTATCGGACGCTGGAGCTGGAGCTGCTCGAGTGGCAGGAGCGGGAGCTTTTTGAGTACTTT
  GTGGTTGTGTCCCTCAAGAAGAAGCCATCTCGGAACACCTACCTCCCTGAAGTCTCCTATCAGTTTCCCA
  AGCTGGACCGTCCCACCAAACAGATGCGGGAGGCAGAAGAAAGGCTCAAAGCTATCCCCCAGTTTTGCTT
  TCCGGATGCCAAGGACTGGCTCCCTGTGTCAGAATACAGCAGTGAAACCTTTTCTTTCATGCTGACTGGG
  GAAGATGGCAGCAGGCGCTTTGGCTATTGTAGGCGCTTACTGCCAAGTGGGAAAGGGCCTCGGTTGCCAG
  AGGTGTACTGTGTCATCAGCCGCCTAGGCTGCTTCGGTTTGTTTTCCAAGGTCCTAGATGAGGTGGAGCG
  CCGGCGTGGGATCTCAGCTGCACTGGTCTACCCCTTCATGAGAAGTCTCATGGAATCGCCCTTTCCAGCC
  CCAGGGAAAACCATCAAAGTGAAGACATTTCTTCCCGGCGCTGGCAATGAGGTGTTAGAGCTGCGTCGGC
  CTATGGACTCCCGCCTGGAGCACGTGGACTTTGAGTGCCTCTTCACCTGCCTCAGTGTGCGTCAGCTTAT
  CCGAATCTTTGCCTCATTGCTGTTGGAACGCAGAGTCATTTTTGTAGCAGATAAGCTCAGTACCCTGTCC
  AGCTGCTCTCACGCGGTGGTGGCCTTGCTCTACCCCTTCTCCTGGCAGCACACATTCATTCCTGTCCTCC
  CAGCCTCCATGATTGACATTGTCTGCTGTCCCACCCCTTTCCTGGTTGGCCTGCTCTCCAGCTCCCTTCC
  CAAACTGAAGGAGCTGCCTGTGGAAGAGGCACTGATGGTGAATCTGGGATCTGACCGATTCATCCGACAG
  ATGGACGACGAGGACACACTGTTACCTAGAAAGTTGCAAGCTGCTCTGGAGCAGGCTCTGGAGAGGAAGA
  GTGAGCTCATCTCCCAGGACTCTGACAGTGACTCTGATGATGAATGTAATACCCTCAATGGACTTGTGTC
  AGAGGTGTTTATCCGGTTCTTTGTGGAGACTGTGGGTCACTACTCCCTCTTCCTGACACACAGTGAGAAG
  GGGGAAAGGGCTTTTCAGCGAGAGGCCTTCCGCAAGTCTGTGGCCTCCAAAAGTATCCGCCGTTTTCTTG
  AAGTTTTTATGGAATCTCAGATGTTTGCTGGCTTCATCCAAGACAGGGAGCTAAGGAAGTGTCGAGCCAA
  GGGCCTCTTTGAGCAGAGAGTGGAACAGTATCTAGAAGAGCTCCCTGACACTGAGCAGAGCGGGATGAAC
  AAGTTTCTCCGGGGCCTGGGCAACAAGATGAAGTTCCTCCACAAGAAGAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029811
• Insert Size: 2154 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029811.2, NP_084087.2
• RefSeq Size: 3004 bp
• RefSeq ORF: 2154 bp
• Locus ID: 76954
• UniProt ID: Q924W7
• Cytogenetics: 7 57.27 cM
• Gene Summary: Isoform 1: May be involved in cytoskeletal organization and tumorogenicity. Seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Plays a role in EGFR trafficking from recycling endosomes back to the cell membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.