Epx (NM_007946) Mouse Untagged Clone

CAT#: MC220986

Epx (untagged) - Mouse eosinophil peroxidase (Epx), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epx
• Synonyms: E; EPO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220986 representing NM_007946
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGCAGCTTCTGGCCCTGGTAGGGGCCTTAGCCACACTCATCCTCACCCAACACGCTGAAGGCA
  CTGCCCCAGCTTCCCCCAGTCCAGTGGAGATTTCAGTCCTTCGAGACTGCATAGCAGAGGCTAAGCTGCT
  GGTGGACACTGCCTACAATCACACACAGAAAAGCATCATGCAACGTCTTCGCAGTGGCTCGGCCAGCCCC
  ATGGACCTCCTGGCCTACTTCAAACAGCCAGTAGCAGCCACCAGAAGAGTGGTTCAGGCCGCTGATTATA
  TGCATGTGGCCTTAGGGCTGCTAGAAGAGAGGCTGCAGCCACGGGGATCTAGACCCTTCAATGCCACTGA
  TGTGCTCACAGAACCACAGCTGCGCCTGCTGTCCCAGGCCAGTGGCTGTGCTCTCCAGGACCAGGCTGAG
  AGGTGCAGCAACAAGTACCGAACCATAACAGGGAGATGCAACAACAAGAAGCATCCCTGGCTGGGAGCCT
  CCAACCAGGCCCTGGCCCGCTGGCTGCCAGCTGAGTATGAAGACCACAGGTCTCTCCCCTTTGGCTGGAC
  CCCTGGAAAGAGGCGTAATGGCTTCCTGCTTCCTCTTGTCCGGGATGTCTCCAATCAGATTGTGCGCTTC
  CCCAGCAAGAAACTGACCTCAGACCGCGGCCGGGCCCTTATGTTCATGCAGTGGGGCCAGTTCATCGACC
  ATGACCTGGACTTCTCCCCGGAGTCCCCAGCCAGAGTGGCCTTCAGCATGGGTGTGGACTGTGAGAAGAC
  CTGTGCCCAGCTGCCCCCCTGCTTCCCCATCAAGATCCCACGCAATGATCCCCGCATCAAGAACCAGCGC
  GACTGCATCCCCTTCTTCCGCTCTGCCCCGGCGTGCCCCCAAAACAGGAACAAAGTCCGCAACCAGATCA
  ACGCACTTACATCCTTCGTGGACGCCAGCATGGTGTATGGCAGCGAGGTGACCCTCGCCCTGCGGCTCCG
  TAATAGGACCAACTTCCTGGGGCTGCTGGCCACGAACCAGCGATTCCAGGACAACGGTCGAGCCCTACTG
  CCTTTTGACAACCTGCATGAGGACCCCTGTCTCCTGACTAACCGCTCTGCACGCATCCCCTGCTTCCTGG
  CAGGTGACACTCGGTCAAGTGAAACCCCCAAACTGACGGCCTTGCACACGCTGTTTGTGAGAGAACACAA
  TCGCCTAGCCGCTGAGCTGAGGCGCCTGAATCCCCACTGGAGTGGAGACAAGCTGTACAACGAAGCCCGG
  AAGATCGTGGGAGCCATGGTCCAGATCATCACCTACCGAGACTTCCTGCCTCTGGTTCTGGGCAGGGCCC
  GGATCAGAAGAACTTTGGGTCCCTACAGAGGGTATTGCTCCAATGTGGACCCCCGTGTAGCCAATGTTTT
  CACCCTGGCCTTCCGCTTTGGCCACACCATGCTTCAGCCCTTCATGTTCCGCCTGGATAGCCAGTATCGG
  GCCTCGGCACCCAACTCCCACGTCCCGCTTAGCTCTGTATTCTTTGCCAGCTGGCGAATCATACATGAAG
  GTGGCATCGACCCTATCCTCCGAGGTCTCATGGCCACCCCAGCTAAGCTGAACCGGCAGGATTCCATGCT
  GGTGGATGAGCTCCGCGACAAGCTGTTTCAGCAAGTGAGAAGGATCGGGCTGGATCTGGCAGCTCTGAAC
  ATGCAGCGCAGCCGGGACCACGGCCTTCCAGGATACAATGCCTGGAGACGCTTCTGTGGGCTTTCCCAGC
  CCCGAAACTTGGCCCAGCTTAGTCGGGTGCTAAAAAATCAGGATTTGGCAAGGAAGTTTCTGAGACTGTA
  CAAGACGCCCGACAACATTGACATCTGGGTCGGAGCCATTGCGGAGCCTCTCCTGCCGGGGGCCCGAGTG
  GGGCCGCTTCTGGCTTGTCTTTTTGAGAACCAGTTCAGGAGAGCCAGAGATGGAGACAGATTCTGGTGGC
  AGAAATGGGGAGTTTTCACCAAAAGACAACGCAAAGCCCTGAGGCGAATCTCTTTATCTCGGATTGTATG
  CGACAATACTGGCATCACCACGGTTTCGAGGGACATCTTCAGGGCTAACATCTACCCACAGGGTTTTGTG
  AGCTGTAGCCGTATTCCCAAGTTGAACCTATCAGCCTGGCGAGGCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007946
• Insert Size: 2151 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007946.2, NP_031972.2
• RefSeq Size: 2691 bp
• RefSeq ORF: 2151 bp
• Locus ID: 13861
• UniProt ID: P49290
• Cytogenetics: 11 52.25 cM
• Gene Summary: This gene encodes a member of the peroxidase superfamily of enzymes that is produced by eosinophils and plays a critical role in eliminating tissue-invasive parasites. The encoded preproprotein undergoes proteolytic processing to generate a heterodimeric enzyme that forms a predominant component of the intracellular granules of eosinophils. Mice lacking the encoded protein exhibit resistance to ulcerative colitis induced by dextran sulfate. [provided by RefSeq, Jul 2016]