Smg5 (BC100408) Mouse Untagged Clone

CAT#: MC220908

Smg5 (untagged) - Mouse Smg-5 homolog, nonsense mediated mRNA decay factor (C, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smg5
• Synonyms: MGC30464, MGC56792, mKIAA1089
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC100408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAAGGCCCTCCTCCAGGGGAGAGCAGCGAACCCGAGGCAAAGGTCCTCCACACCAAGCGGCTTT
  ACCGGGCTGTGGTGGAGGCTGTGCATCGGCTAGATCTCATCCTTTGCAACAAAGCTGCTTATCAGGAAGT
  GTTCAAACCAGAGAACGTTAGCCTGAGGAACAAGCTACGAGAGCTCTGTGTGAAGCTTATGTTCTTGCAT
  CCAGTAGACTATGGGAGGAGGGCTGAGGAACTGCTGTGGAGAAAGGTATACTATGAAGTTATCCAACTTA
  TCAAGACTAACAAAAAGCACATCCACAGTCGGAGCACCTTGGAATGTGCCTACAGGACTCATCTGGTCGC
  TGGCATTGGCTTCTACCAGCATCTCCTTCTCTATATCCAGTCCCACTACCAGCTGGAACTACAGTGCTGC
  ATCGACTGGACTCACGTCACCGATCCCCTCATGGGATTCAAGAAGCCAGTATCTGCTTCAGGAAAGGAGA
  TGGATTGGGCACAAATGGCTTGCCACCGATGCCTGGTGTACCTGGGGGATCTGTCACGCTATCAGAATGA
  GTTGGCTGGTGTGGACACCGAGCTGCTAGCTGAGAGATTCTACTATCAAGCCTTGTCAGTGGCTCCCCAG
  ATTGGAATGCCCTTCAATCAGCTGGGTACCCTTGCAGGCAGCAAGTATTACAACGTGGAAGCCATGTATT
  GCTACTTGCGCTGCATCCAGTCGGAAGTGTCCTTTGAGGGAGCCTATGGGAACCTCAAGAGACTATATGA
  CAAGGCAGCCAAAATGTACCACCAGTTGAAGAAGTCTGAGACCAGGAAGCTCTCCCCTAGCAAAAAGCGA
  TGCAAAGACATTAAGAGGTTGTTGGTGAACTTCATGTACCTACAAAGCCTGTTACAGCCCAAAAGTAGCT
  CCGTGGACTCAGAACTGACCTCACTCTGCCAGTCAGTCCTAGAGGATTTCAACCTCTGCCTCTTCTACCT
  GCCCTCCTCACCCAACCTTGGTCTAACCAACGAGGATGAGGAGGAGTGTGAAAGTGGATATGCTTTCCTC
  CCCGACCTGCTCATCTTTCAGATGGCCATCATCTGCCTCATGGGTGTGCACAGTTTAAAGAGAGCAGGAT
  CCAAGCATTATAGTGCAGCCATTGCTTTCACCCTGGCCCTCTTTTCCCACCTTATCAATCATGTCAACAT
  ACGGCTGCAGGCGGAACTGGAAGAGGGCGAGAACCCTGTCTCAGCTTTTCAGAGTGATGGCACAGATGAA
  CCAGAGTCAAAAGAAGCACTAGAAAAAGAGGAGCCAGAGCCTGAGCCTCCCACTGTGGTACCCCAAGCTG
  ATGAGGGCAGAAAGAGCCGTAAGCACTCCCGACTCTCTTGTCTACGTCGTCGCCGCCGCCACCATCCTCC
  TAAAGCTGGTGATGACAGTGACCTGAGTGAGGGTTTTGAATCTGACTCTAGCCACGACTCTGCCCAGGCC
  AGTGACGGCTCAGACAGTGGCTCTGATAAGAGCCTGGAAGGCAGGGGCACTGCTTTTGATGCAGAGACAG
  ACTCAGAAATGAACAGCCAGGAGTCCCGGTCAGACCTGGAAGATATGGAGGATGAGGAGGGGACAAGATC
  TCCAGCCCAGGAGCCCCCTCAGGCCAGATCAGAGGTTCCAGATTCCCTCAATGGCCCCCTGGGCCCCAGT
  GAAGCAAGCATTGCCAGCAATTTACAAGCCATGTCCACCCAGATGTTCCAGACCAAGCGCTGCTTCCGAC
  TGGCTCCCACTTTCAGCAACCTGCTCCTGCAGCCCACCACGGAACCTAACAGTGTGGCCAGTCACAGGCC
  TTGTGTCAACGGGGATATGGACAAGCCTTTAGAACCAGGTGCGTGGGTGCATGAGAGTCCACCTTCTCGC
  AGAGTAACCTCACCCTTGGGGTTTTGCTGTCACTGTGGAATCCTTGTCCTGTGCACGTACTCATTTCCAG
  CCTCCAGAACGTTTGCTGTCTTGCTGCTTTCCACTGCAGACTGCTCCAGCTTCACTCTCTTCTCTCCTCA
  TATTGGAGGTTGTACTTCCTTCCCTCATGGCATCCAGCTTCCTGAAGGCTCCCAGAAGCTGGGCATAATG
  TTCCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC100408
• Insert Size: 2109 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC100408, AAI00409
• RefSeq Size: 3121 bp
• RefSeq ORF: 2108 bp
• Locus ID: 229512
• Cytogenetics: 3 F1
• Gene Summary: Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity (By similarity).[UniProtKB/Swiss-Prot Function]