BC002230 (BC025577) Mouse Untagged Clone

CAT#: MC220899

BC002230 (untagged) - Mouse cDNA sequence BC002230 (cDNA clone MGC:38228 IMAGE:5323598), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: BC002230
• Synonyms: 6720454P05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025577
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGCTCTTTCTTCAGCAGTGCCACTTTCTACGGCAGGCTGGCCACTCAGAGAAGGTCATCTCTT
  TGTTCCAGGCCATGGTTGACTTCACCTTCTTCAAGCCTGACAGTGTGAAAGAGCTGCCTACTAAAGTACA
  GGTGGAATTCTTTGAGCCCTTCTGGGACAGCGGAGAACCACGCGTTGGGGAGAAGGGAGCCCGAGGCTGG
  CGGGCGTGGATGCACCAACAGGAGCGAGGTGGCTGGGTGCTCATCACCCCAGATGAGGATGACGAGGAGC
  CCGAAGAGGAGGACCAGGAGATAAAAGACAAGACCCTGCCCCGGTGGCAGATCTGGCTTGCTGTTGAGCG
  TTCTCGAGACCAGAGGCACTGGCGTCCCTGGCGCCCTGATAAGACCAAGAAGCAAACGGAGGAAGACTGT
  GAGGACCCTGAGAGACAGGTGCTGTTTGATGACATTGGGCAATCTTTGATCAGACTTTCCAGCCCAGACC
  TTCAGTTCCAGCTCATCCAGGCCTTTCTGCAGTTCCTGGGCGTGCCTTCCGGCTTCCTGCCCCCAGCCTC
  CTGCCTTTACCTGGCCATGGACGAGAGCAGCATCTTTGAGAGTGAACTTTATGATGAAAAGCCCCTGACT
  TACTTCAACCCTTCCTTTTCGGGCATTAGCTGTGTCGGCTCCATGGAACAGTTGGGTCACCCTCGCTGGA
  CCAAAGGTCACAACCGAGAGGGCGAGGAGTTTGTCCGCAATGTCTTCCACCTGGTGCTGCCTTTGCTCGC
  AGGCAAGCAGAAGTCCCAGGTCTGCCTCTCCTGGTTACGGTACGAGATTGCAAAGGTCATTTGGTGTCTA
  CACACTAAAAAGAAGCGATTGAAGTCTCAGGGAAAGAGCTGCAAAAAACTAGCCAAGAATCTCCTCAAGG
  AGCCAGAAAACCGCAACAACTTCTGCCTCTGGAAGCAGTATGCACACCTGGAGTGGTTGCTCGGTAACAC
  AGAGGATGCCAGAAAAGTGTTCGACACAGCACTCAGCATGGCAGGCAGCAGCGAGCTGAAGGACCGTGAA
  CTCTGCGAGCTCAGTCTGCTCTATGCCGAGCTGGAGATGGAACTGTCGCCGGACTCAAGAGGAGCCACCA
  CAGGCCGAGCGGTCCACATATTAACACGACTGACGGAGAGCAGTCCCTACGGGCCCTACACGGGGCAGGT
  CTCGTCCACCCAGGTGCTGAAAGCTCGGAAGGCTTACGAGCTCGCGCTGCAGGACTGCCTGGGCCAGAGC
  TGTGCCTCCAGCCCAGCTCCTGCAGAGGCCTTGGACTGCCTGGGTAGCTTGGTCCGATGCTTTATGCTCT
  TCCAGTATTTGACTGTGGGCATTGATGCTGCTGTGCAGATATACGGAAGGGTGTTTGCCAAGCTGAAGGG
  CTCTGCTCGCTTAGAAGACCCAGGGCCAGAGGACAGCACCAGCTCCCAGAGTTTGACCAATGTGCTCGAG
  GCTGTCAGCATGATGCACACAAGCCTGCTGCGATTCCACATGAACGTTTGTGTTTACCCTCTGGCTCCAC
  TCCGAGAGACCCTCTCCGATGCTTTAAAGCTGTATCCGGGCAACCAGGTTCTTTGGAGGGCTTATGTACA
  GATTCAGAATAAGTCCCACAGTGCTAACAAGACCAGAAGGTTCTTTGACACAGTTACTAGGTCTGCCAAA
  CATTTGGAGCCTTGGCTATTTGCAATCGAAGCTGAGAAGCTGAGGAAAAAGCTAGTGGAATCTGTTCAGA
  GGGTAGGAGGCAGAGAGGTCCATGCAACAATCCCCGAGACTGGCCTTACGCATCGCATCAGAGCTTTGTT
  TGAAAATGCGATTCGGAGCGACAAGGGCAACCAGTGTCCCCTACTGTGGAGGATGTATTTGAATTTTTTG
  GTTTCCTTAGGAAACAAAGAAAGAAGCAAAGGCGTGTTCTACAAGGCCCTCCAGAGCTGTCCTTGGGCAA
  AGGTGCTATACATGGACGCCATGGAGTATTTCCCAGACGAGCTGCAGGAGATCCTGGACGTGATGACCGA
  GAAGGAGCTCCGTGTGCGCCTGCCCCTTGAGGAGCTGGAGCTGCTGCTGGAGGATTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: BC025577
• Insert Size: 2088 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025577, AAH25577
• RefSeq Size: 2358 bp
• RefSeq ORF: 2087 bp
• Locus ID: 217827
• Cytogenetics: 12 E
• Gene Summary: Protein of the nuclear speckles that regulates RNA degradation and export from the nucleus through its interaction with MTREX an essential factor directing various RNAs to exosomal degradation. Changes the conformation of MTREX, precluding its association with the nuclear exosome and interaction with proteins required for its function in RNA exosomal degradation. Negatively regulates, for instance, the degradation of mRNAs and lncRNAs by inhibiting their MTREX-mediated recruitment to nuclear exosome. By preventing the degradation of RNAs in the nucleus, it promotes their export to the cytoplasm.[UniProtKB/Swiss-Prot Function]