Lca5 (NM_027448) Mouse Untagged Clone

CAT#: MC220846

Lca5 (untagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lca5
• Synonyms: 4930431B11Rik; 5730406O13Rik; AV274874; ORF64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220846 representing NM_027448
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAAAGAGCCCGAAGTCCAGATATTGAACAAGGAAAAAAAGGAGGCAAACACCCATACTCTCATT
  ATTCGTCTGACTTTGGGTCCTCACCACAGTCCTCTGGCCCCTCATCACCTGTCAACACGACGCCATGTGC
  CAGTACTAGGGAGAAGAATCCCAAAAGACACTTGTCAGACAACCAAGTGCATCACCCAACCGTTAGGAAA
  GTAAGTCCTAAGGCTGTACCCAGCAAAAAGGGCATCCGAGTGGGATTTCGGTCCCAAAGCCTCAACAGAG
  AGCCACTTCGGAAAGATCCAGATATCGTAACAAAGCGGGTTCTTTCTGCCAGACTTCTGAAAATCAACGA
  GCTGCAGAACGAAGTGTCAGAACTACAGGTCAAGTTAGCCCAGCTGCTCAAAGAGAACAAGGCTTTGAAA
  AGCCTTCAGTATCGTCAGGAGAAAGCGCTGAATAAGTTTGAAGATGCGGAGAACGAGATCTCACAACTTA
  TACACCGCCATAACAATGAGATTACTGCGCTCAAAGAACGCTTACGAAAATCTCAGGAGAAGGAGCGGGC
  GACAGAGAAAAGAGTGAAAGAGACAGAAGGTGAACTATTTAGGACGAAATTTTCCTTACAGAAACTGAAA
  AAGATCTCCGAGGCCAGACACCTACCGGAGCGAGACGATTTGGCCAAGAAACTGGTTTCGGCAGAGTTAA
  AATTAGATGACACGGAGAGAAAGATTAAGGAGCTATCGAAGAACCTGGAGCTGAGCACGAACAGTTTCCA
  GCGGCAGCTGCTCGCTGAAAGGAAAAGAGCATTCGAGGCTTACGATGAAAATAAAGTTCTTCAAAAGGAG
  CTGCAGCGGCTCCATCACAAGCTCAAGGAAAAGGAGAAAGAATTGGATATAAAAAACATCTATGCTAATC
  GCCTGCCCAAATCCTCTCCAAAGAAAGAGAAAGAAATCGAAAGGAAACATGTATCCTGCCAGAGTGACTT
  TACAGACCAGTGTACAAAAGGAGTACAGACCGCTGAAGACTTTGAGCTGGAGGACTTTCCTTTCACAGCC
  CAGACAGTTCTCTGCTATGAAAACAGATGGGATGAACCAGAATATCTCTCTTCGTACCTGGAGTACCAGG
  ATCTCAACAAACATGGATCAGAGATGCTAAGTTCAGTGTTGGGACAAGAAGGAAAGTATGATGAAGATGA
  GGACCCATGCTCTGCAAAGCAGGAGGCCAGGAAGCCTGAGAGTGAATGGGCAAGAGAAGAACTTGATAAG
  GTGAAAGGCAAGTCAGCTTTGCTAGGGAGAGCAGAGAAGCTGGCATTGGAAGCTGGGAGATTCCCAACGG
  AAAATTATCAAGCTCAGTCTGTCGATAAATTCGAAGATGAGGCAGAAAGACTAAAGACAGAAATGCTCCT
  TGCCAAACTGAACGAAATCAACAAAGAACTCCAAGATCCTCAGAACCTCGGGCGGGCCCCTTTGCCATTG
  CTGCCCAACTTCGAATCCAAGCTGCACTCTCCAGACAGAAGTACCAGACCTTACTCGTTCCCCGAGTCCT
  TAGACCGATCGTTTAATGGACAACACTTGCAGGACTTGAGTTTTTTAACTCCCAGGGGAGAAGGCGGGAG
  TCCAGGACCGATCCGAAGTCCAGGACAGATCCGAAGTCCAGCCCCTCTAGATGAGTTTTCATTTGGTAGC
  TATGTGCCTTCGTTTGGAAAAACATTAGGGAAGTCAAATCCACCTAGCCAAAAAAGTAGCCTTTTAGATT
  TCCAAAGCAACAGTTCTGAAAGCCCGAGTAAAGACAGTCTTGATTTAATGTCGAGGAAAGAGAAAAAGGC
  GACTCTGATGGAGCAGCTGTTTGGCCCCAGCGCCAGCAACACCAGTGTCTCCTCCAAGAGCACCGACCCA
  CACTTCCCTGCTGCCAGCAGAGGTGACATGGACCCTCTTCATTTCCTCTCTGGGGACAGAAACAGCAGAG
  TCCGGGAGCCTGGCGATGAAGAGGAAGACCTTTTCCTCAGGGAAGGAAGAAGTTTTAATCCAAATAGACA
  CCGGTTGAAGCACGCAAGCAATAAGCCAACAGTCACGGCCGTTGACTCTGTTGATGAGGACATTGAAGAG
  GTTACCCTCAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027448
• Insert Size: 2115 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027448.2, NP_081724.1
• RefSeq Size: 4235 bp
• RefSeq ORF: 2115 bp
• Locus ID: 75782
• UniProt ID: Q80ST9
• Cytogenetics: 9 E2
• Gene Summary: Involved in intraflagellar protein (IFT) transport in photoreceptor cilia.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer protein (isoform b).