Fbxw7 (NM_001177774) Mouse Untagged Clone

CAT#: MC220783

Fbxw7 (untagged) - Mouse F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxw7
• Synonyms: 1110001A17Rik; AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxo30; Fbxw6; SEL-10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220783 representing NM_001177774
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGAGGGAATGCTT
  CCTCAAGCCAGGTTGATGAGGGACAGATGAATCGCGTGGTTGAGGAGGATCCACAGCAGCAAGCGAGACA
  TCAAGAGGAGGAGCACACTGCGCGGAATGGTGAACTTGTGGGTGCAAACCCTAGGCCTGGAGACCAGAAC
  GATACCCAGCAAGGACAAGTGGAGGAAAATAATAACCGCTTTATTTCAGTAGATGAGGACTCTTCGGGAA
  ATCAGGAAGAGCAAGAGGAAGATGAAGAGCATGCTGGGGAACAGGAGGAGGAAGAGGAGGAAGAGGAAGA
  GGAGGAGGAGATGGACCAGGAGAGTGATGATTTTGATCCGTCTGATGACAGTAGCAGAGAAGATGAACAT
  ACGCACAATAGCAATGTCACAAACTGCAGTAGTGTCTCGGACCTGCCCGCTCACCAGCTCTCCTCTCCAT
  TCTATACAAAGACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAAGTTCGTTCCTTTTCTTTGGG
  AAAGAAACCATGCAAAGTCTCAGATTATACCAGTACCACTGGCCTTGTACCATGTTCAGCAACACCAACA
  ACTTTTGGGGACCTGAGAGCAGCCAATGGGCAAGGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCAC
  CCACAGGCCTTCAAGAGTGGCTGAAAATGTTTCAGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGA
  TGAGCTCATTGACAGCTGTGAACCAACACAAGTGAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAG
  CGAGACTTCATCTCCTTGCTTCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACC
  TGCTGCAAGCGGCTCAGACTTGTCGATACTGGAGAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAA
  ATGTAAAGAAGAGGGGATTGATGAACCGTTGCACATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATA
  CACAGCCCATGGAAGAGTGCGTATATCAGACAGCACAGAATTGATACAAACTGGAGACGAGGAGAACTCA
  AATCTCCTAAGGTGCTGAAAGGGCATGATGACCATGTGATCACATGCCTACAGTTTTGTGGCAACCGCAT
  AGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTA
  GTGGGACATACAGGTGGAGTGTGGTCATCACAGATGAGAGACAATATCATCATCAGTGGATCGACTGACC
  GGACTCTCAAAGTGTGGAATGCTGAAACTGGAGAGTGTATACATACTTTATATGGGCACACTTCTACTGT
  ACGGTGTATGCATCTCCATGAAAAAAGGGTTGTAAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGAT
  ATTGAGACCGGCCAGTGTTTACACGTCCTGATGGGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATG
  GCAGGAGGGTTGTTAGTGGAGCTTATGATTTTATGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCT
  ACACACGTTACAGGGACACACTAATAGAGTCTATTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGA
  TCTCTTGATACATCAATCCGAGTCTGGGATGTGGAGACAGGGAATTGTATTCACACGCTAACAGGACACC
  AGTCATTAACGAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAA
  GATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAGGTCCCAGCAAGCATCAGAGCGCTGTG
  ACCTGCTTACAGTTCAACAAGAACTTCGTAATTACCAGCTCAGACGACGGAACGGTCAAACTCTGGGACT
  TGAAAACGGGTGAATTTATCCGAAACCTCGTCACATTGGAGAGTGGGGGGAGCGGGGGAGTTGTGTGGCG
  GATCAGGGCCTCAAACACAAAGCTGGTGTGTGCAGTCGGGAGTCGGAATGGAACTGAGGAAACCAAGCTC
  CTGGTGCTGGACTTTGATGTGGACATGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177774
• Insert Size: 2133 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177774.1, NP_001171245.1
• RefSeq Size: 4092 bp
• RefSeq ORF: 2133 bp
• Locus ID: 50754
• UniProt ID: Q8VBV4
• Cytogenetics: 3 37.7 cM
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21953459, PubMed:22748924). Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:22748924). Mediates ubiquitination and subsequent degradation of CCNE1 and MYC (PubMed:22748924). Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1 (By similarity). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity). SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (PubMed:21953459). Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593). Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination (PubMed:27238018).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) and variant 2 encode the same isoform (1).