Aloxe3 (NM_011786) Mouse Untagged Clone

CAT#: MC220771

Aloxe3 (untagged) - Mouse arachidonate lipoxygenase 3 (Aloxe3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aloxe3
• Synonyms: e-LOX-3; eLOX-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220771 representing NM_011786
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTATATCGGCTGTGTGTGACCACTGGTTCCTACCTGAAGGCTGGCACATTGGATAACATCTATG
  CTACATTGGTAGGCACCTGTGGTGAAAGCCCTAAGCAGAAGCTGGATCGCGTAGGCAGGGACTTCGCCTC
  TGGATCGGTTCAGAAGTATAAGGTGCGCTGTGAAGCAGAGCTGGGTGAGATCTTGCTGTTGCGCTTACAC
  AAGGAGCGCTTTGCTTTCTTCTGCAAGGACCCTTGGTACTGCAGTCGAATCTGTGTCACTGCCCCCGATG
  GCTCTGCTGTCCACTTTCCCTGCTACCAGTGGATCGATGGCTACTGTACTGTGGAGCTGCGGCCAGGAAC
  AGCAAGAACCATCTGTCAGGATTCTCTTCCCCTCCTTCTGGACCACAGGAAACGGGAACTCCAAGCCCGC
  CAAGAATGTTATCGCTGGAAGATCTTCGCCCCTGGCTTCCCTCGGATGGTGGATGTCAGCAGCTTTCAGG
  AGATGGAGTCAGATAAGAAATTTGCCTTGACCAAGACAGTACCTTGTGCAGAACAGGATGACAACTCTGG
  GAACCGGTACCTGCCTGGCTTTCCTATGAAGATTGACATCCCATCTCTGCTGCACATGGAACCCAACATT
  CGATACTCAGCCACCAAGACGGCCTCACTGATCTTCAACGCCCTTCCTGCATCCTTTGGCATGAAGATTC
  GAGGTCTCCTGGACCGCAAGGGCTCCTGGAAGAGGCTGGACGACATCCGGAACATCTTCTGGTGCCATAA
  GACCTTCACTTCAGAGTACGTCACCGAGCATTGGTGTGAGGACAGCTTCTTTGGGTACCAGTACCTGAAT
  GGTGTCAACCCTGTCATGCTTCATTGCCTCTCCAGCTTGCCCAGCAAGCTGCCTGTCACCAATGACATGG
  TGGCACCTTTGCTGGGACCAGGCACCTGCCTACAAACAGAGCTAGAGAGGGGGCACATCTTCCTGGCGGA
  CTACTGGATCCTGGCGGAGGCCCCAGTCCATTGCATAAACGGTCTCCAACAGTACGTAACAGCGCCCCTC
  TGCCTGTTGTGGCTCAACCCACAGGGGGTGCTGCTGCCATTGGCAATCCAGCTCAGCCAGACACCAGGGC
  CAGAGAGCCCCATCTTTCTGCCCACTGATTGCGAGTTGGACTGGCTGCTGGCCAAGACGTGGGTGCGCAA
  CTCTGAGTTTTTGGTGCACGAGAACAACACGCATTTTCTGTGCACGCATTTGCTATGCGAGGCCTTCTCC
  ATGGCTACACTGCGTCAGCTGCCGCTCTGTCATCCAGTCTACAAGCTCCTGCTTCCTCATACTCGCTACA
  CGCTGCAAGTGAACACCATCGCAAGAGCCACGCTGCTCAACCCAGACGGCCTCGTGGACAAGGTCACGTC
  CATCGGTAGGCAGGGCCTCATCTACCTCATGAGCACCGGGCTGGCCCACTTCACCTACACGGATTTCTGC
  CTACCGGATAGCATACGGGCTCGTGGCGTCCTGACCATTCCCAACTACCACTACCGAGACGACGGCCTGA
  AGATCTGGGCGGCTATTGAGAGGTTTGTCTCAGAGATTGTGAGTTACTATTATCCCAGCGATGCGTCTGT
  GCAGCAGGACTGTGAACTGCAGGCCTGGGTGGGTGAGATTTTTGCTCAGGCGTTCCTCGGACGGGAAAGT
  TCAGGCTTCCCAAGCCGGCTGTGCACCCCAGGAGAGCTCGTGAAGTATCTCACCGCAATCATCTTTAATT
  GCTCCGCCCAGCACGCTGCGGTCAATAGTGGGCAGCATGACTTTGGGGCCTGGATGCCCAACGCCCCATC
  ATCCATGAGGCAGCCTCCGCCTCAGACCAAGGGCGACACGACAATGAAGAGTTACCTAGACACCCTCCCA
  GAGGTGAACACCACCTGTAGAAACCTTCTCCTCTTCTGGCTGGTCAGCCAAGAGCCAAAGGACCAGAGAC
  CTCTGGGCACCTACCCAGATGAACACTTCACAGAGGAGGCCCCACGGCAGAGCATCGCAGCCTTCCAGAA
  CTGCCTGGCCCAGATCTCAAAGGACATCAGGGAGCGCAACCAGAGCCTGGCACTGCCCTATGCCTACTTG
  GATCCTCCACTCATTGAGAACAGTGTTTCCATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011786
• Insert Size: 2136 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011786.2, NP_035916.2
• RefSeq Size: 2538 bp
• RefSeq ORF: 2136 bp
• Locus ID: 23801
• UniProt ID: Q9WV07
• Cytogenetics: 11 42.38 cM
• Gene Summary: Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.[UniProtKB/Swiss-Prot Function]