Mapk8ip1 (NM_011162) Mouse Untagged Clone

CAT#: MC220748

Mapk8ip1 (untagged) - Mouse mitogen-activated protein kinase 8 interacting protein 1 (Mapk8ip1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk8ip1
• Synonyms: IB1; JIP-1; Jip1; mjip-2a; Prkm8ip; Skip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220748 representing NM_011162
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCGAGAGAGCGGCCTGGGCGGGGGCGCCGCGTCCCCACCGGCCGCTTCCCCATTCCTGGGAC
  TGCACATCGCGTCGCCTCCCAATTTCAGGCTCACCCATGACATCAGCCTGGAGGAGTTTGAGGATGAAGA
  CCTTTCGGAGATCACTGACGAGTGTGGCATCAGCCTGCAGTGCAAAGACACCCTGTCTCTCCGGCCCCCG
  CGCGCCGGGCTGCTGTCTGCGGGTAGCAGCGGCAGCGCGGGGAGCCGGCTGCAGGCGGAGATGCTGCAGA
  TGGACCTGATCGACGCGGCAGGTGACACTCCGGGCGCCGAGGACGACGAGGAGGAGGAGGACGACGAGCT
  CGCTGCCCAACGACCAGGAGTGGGGCCTCCCAAAGCGGAGTCCAACCAGGATCCGGCGCCTCGCAGCCAG
  GGCCAGGGCCCGGGCACAGGCAGCGGAGACACCTACCGACCCAAGAGGCCTACCACGCTCAACCTTTTCC
  CGCAGGTGCCGCGGTCTCAGGACACGCTGAATAATAACTCTTTAGGCAAAAAGCACAGTTGGCAGGACCG
  TGTGTCTCGATCATCCTCCCCTCTGAAGACAGGAGAACAGACGCCTCCACATGAACACATCTGCCTGAGT
  GATGAGCTGCCACCCCAGGGCAGTCCTGTTCCCACCCAGGACCGCGGCACTTCCACCGACAGCCCTTGTC
  GCCGAAGTGCAGCCACCCAGATGGCACCTCCAAGCGGTCCCCCTGCCACTGCGCCTGGTGGCCGGGGCCA
  CTCCCATCGAGACCGAATCCACTACCAGGCAGATGTGCGGCTCGAGGCGACTGAGGAGATCTACCTGACC
  CCAGTGCAGAGGCCCCCAGACCCTGCAGAACCCACCTCCACCTTCATGCCACCCACGGAGAGCCGGATGT
  CAGTTAGCTCCGATCCAGACCCTGCCGCTTACTCTGTAACTGCGGGGCGGCCACACCCCTCCATCAGTGA
  AGAGGATGAGGGCTTCGACTGCCTGTCATCCCCAGAGCGAGCTGAGCCACCAGGTGGAGGGTGGCGGGGA
  AGCCTCGGGGAGCCACCACCGCCTCCACGGGCCTCACTGAGCTCGGACACCAGCGCACTGTCCTACGACT
  CGGTCAAGTACACACTGGTGGTGGATGAACATGCCCAGCTTGAGTTGGTGAGCCTGCGGCCGTGCTTTGG
  AGATTACAGTGACGAAAGCGACTCTGCCACTGTCTATGACAACTGTGCCTCTGCCTCCTCGCCCTACGAG
  TCAGCCATTGGTGAGGAGTATGAGGAGGCCCCTCAGCCCCGGCCTCCCACCTGCCTCTCAGAGGACTCCA
  CCCCGGATGAGCCTGATGTCCACTTCTCTAAGAAGTTTCTGAATGTCTTCATGAGTGGCCGCTCTCGTTC
  CTCCAGTGCTGAGTCCTTTGGGCTGTTCTCCTGCGTCATCAATGGGGAGGAGCATGAGCAAACCCATCGG
  GCTATATTCAGGTTTGTGCCTCGGCATGAAGATGAACTTGAGCTGGAAGTGGATGACCCCCTGCTGGTGG
  AGCTGCAGGCAGAAGACTATTGGTATGAGGCCTATAACATGCGCACCGGAGCCCGCGGGGTCTTCCCTGC
  CTACTATGCCATTGAGGTCACCAAGGAGCCTGAGCACATGGCAGCCCTTGCCAAAAACAGCGACTGGATT
  GACCAGTTCCGGGTGAAGTTCCTGGGGTCTGTCCAGGTTCCTTATCACAAGGGCAATGATGTCCTCTGTG
  CTGCTATGCAAAAGATCGCCACCACCCGCCGGCTCACCGTGCACTTTAACCCGCCCTCCAGCTGTGTCCT
  TGAGATCAGTGTCAGGGGTGTCAAGATAGGCGTCAAAGCTGATGATGCTCTGGAGGCCAAGGGAAATAAA
  TGTAGCCACTTCTTCCAGCTAAAGAACATCTCTTTCTGTGGATACCATCCAAAGAATAACAAGTACTTTG
  GGTTTATCACTAAGCACCCTGCTGACCACCGGTTTGCCTGCCATGTCTTTGTGTCTGAAGATTCCACCAA
  AGCCCTGGCGGAGTCTGTGGGGCGTGCATTTCAGCAGTTCTACAAGCAGTTTGTGGAGTATACCTGTCCT
  ACAGAAGATATCTACTTGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011162
• Insert Size: 2124 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011162.5, NP_035292.2
• RefSeq Size: 2954 bp
• RefSeq ORF: 2124 bp
• Locus ID: 19099
• UniProt ID: Q9WVI9
• Cytogenetics: 2 E1
• Gene Summary: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity). Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells (PubMed:23963642).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.