Fgfr2 (BC091652) Mouse Untagged Clone

CAT#: MC220722

Fgfr2 (untagged) - Mouse fibroblast growth factor receptor 2 (cDNA clone MGC:102519 IMAGE:5349249), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgfr2
• Synonyms: KGFRTr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC091652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCTGGGGGCGCTTCATCTGCCTGGTCTTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCT
  CCTTCAGTTTAGTTGAGGATACCACTTTAGAACCAGAAGGAGCACCGTACTGGACCAACACCGAGAAGAT
  GGAGAAGCGGCTCCACGCTGTCCCTGCCGCCAACACTGTGAAGTTCCGCTGTCCGGCTGGGGGGAATCCA
  ACGCCCACAATGAGGTGGTTAAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTATAAGG
  TACGAAACCAGCACTGGAGCCTTATTATGGAAAGTGTGGTCCCGTCAGACAAAGGCAACTACACCTGCCT
  GGTGGAGAATGAATACGGGTCCATCAACCACACCTACCACCTCGATGTCGTTGAACGGTCACCACACCGG
  CCCATCCTCCAAGCTGGACTGCCTGCAAATGCCTCCACGGTGGTCGGAGGGGATGTGGAGTTTGTCTGCA
  AGGTTTACAGCGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGG
  GCCTGATGGGCTGCCCTACCTCAAGGTCCTGAAGCACTCGGGGATAAATAGCTCCAATGCAGAAGTGCTG
  GCTCTGTTCAATGTGACGGAGATGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGG
  CCAACCAGTCTGCCTGGCTCACTGTCCTGCCCAAACAGCAAGCGCCTGTGAGAGAGAAGGAGATCACGGC
  TTCCCCAGATTATCTGGAGATAGCTATTTACTGCATAGGGGTCTTCTTAATCGCCTGCATGGTGGTGACA
  GTCATCTTTTGCCGAATGAAGACCACGACCAAGAAGCCAGACTTCAGCAGCCAGCCAGCTGTGCACAAGC
  TGACCAAGCGCATCCCCCTGCGGAGACAGGTAACAGTTTCGGCCGAGTCCAGCTCCTCCATGAACTCCAA
  CACCCCGCTGGTGAGGATAACAACGCGTCTGTCCTCAACAGCGGACACCCCGATGCTAGCAGGGGTCTCC
  GAGTATGAGTTGCCAGAGGATCCAAAGTGGGAATTCCCCAGAGATAAGCTGACGCTGGGCAAACCCCTGG
  GGGAAGGTTGCTTCGGGCAAGTAGTCATGGCTGAAGCAGTGGGAATCGATAAAGACAAACCCAAGGAGGC
  GGTCACCGTGGCAGTGAAGATGTTGAAAGATGATGCCACAGAGAAGGACCTGTCTGATCTGGTATCAGAG
  ATGGAGATGATGAAGATGATTGGGAAACATAAGAACATTATCAACCTCCTGGGGGCCTGCACGCAGGATG
  GACCTCTCTACGTCATAGTTGAATATGCATCGAAAGGCAACCTCCGGGAATACCTCCGAGCCCGGAGGCC
  ACCTGGCATGGAGTACTCCTATGACATTAACCGTGTCCCCGAGGAGCAGATGACCTTCAAGGACTTGGTG
  TCCTGCACCTACCAGCTGGCTAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATCCATCGAGATTTGG
  CTGCCAGAAACGTGTTGGTAACAGAAAACAATGTGATGAAGATAGCAGACTTTGGCCTGGCCAGGGATAT
  CAACAACATAGACTACTATAAAAAGACCACAAATGGGCGACTTCCAGTCAAGTGGATGGCTCCTGAAGCC
  CTTTTTGATAGAGTTTACACTCATCAGAGCGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTTA
  CTTTAGGGGGCTCACCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTCAAAGAGGGACACAG
  GATGGACAAGCCCACCAACTGCACCAATGAACTGTACATGATGATGAGGGATTGCTGGCATGCTGTACCC
  TCACAGAGACCCACATTCAAGCAGTTGGTCGAAGACTTGGATCGAATTCTGACTCTCACAACCAATGAGG
  AATACTTGGATCTCACCCAGCCTCTCGAACAGTATTCTCCTAGTTACCCCGACACAAGGAGCTCTTGTTC
  TTCAGGGGACGATTCTGTGTTTTCTCCAGACCCCATGCCTTATGAACCCTGTCTGCCTCAGTATCCACAC
  ATAAACGGCAGTGTTAAAACATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
  TCCTGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: BC091652
• Insert Size: 1635 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC091652
• RefSeq Size: 4271 bp
• RefSeq ORF: 2123 bp
• Locus ID: 14183
• Cytogenetics: 7 F3
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.[UniProtKB/Swiss-Prot Function]