Clcn4 (BC005553) Mouse Untagged Clone

CAT#: MC220642

Clcn4 (untagged) - Mouse chloride channel 4-2 (cDNA clone MGC:6024 IMAGE:3586154), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clcn4
• Synonyms: Clc4-2; Clcn4-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005553
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTCCTCGAGGAGCCCTTCCCTGACGTGGGGACCTACGAGGACTTCCACACCATAGACTGGCTGA
  GGGAAAAGTCCCGGGATACCGACAGACATAGGAAGATCACCAGCAAAAGTAAGGAGTCTATTTGGGAGTT
  CATCAAGAGCCTGCTGGACGCGTGGTCGGGATGGGTGGTGATGCTACTCATTGGGCTGCTGGCAGGTACC
  TTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGGACCTCAAGGAGGGGGTCTGTCTGTCCGCAT
  TCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGAGACCACTTTTGAGGACAGGGACAAGTGTCC
  CCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCAGAGGGCGCCAGCGCTTACATTCTGAATTAC
  TTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGGCTGTCTCCCTGGTACGTGTGTTCGCACCGT
  ATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTTGAGTGGCTTTATCATCAGGGGCTACTTGGG
  GAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTCGTCGTATCCTCTGGCCTGAGCCTTGGCAAA
  GAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTATAGCA
  AGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGCTGCTGCTGGTGTCTCTGTGGCCTTTGGTGC
  TCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGTTACTACTTTCCCTTGAAAACCTTGTGGAGG
  TCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCTCCATCAACCCCTTTGGAAATAGCCGCCTGG
  TTCTCTTTTACGTGGAGTATCATACACCCTGGAGGCGTAAGACCACCAGGCTGGGCAGGTACCCAGTGTT
  GGAGGTTATTGCGGTGACAGCCGTCACCGCCATCGTGGCCTACCCCAATCCCTACACTCGCCAGAGCACC
  AGTGAGCTCATCTCTGAGCTCTTCAACGATTGTGGGGCTCTCGAGTCTTCTCAGCTCTGTGACTACATCA
  ACGACCCCAACATGACTCGGCCTGTGGATGACATTCCGGACCGGCCGGCTGGGGTTGGAGTTTACACAGC
  CATGTGGCAGCTGGCCTTGGCACTGATCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATT
  CCCTCAGGTCTCTTCATCCCCAGTATGGCTGTCGGAGCCATGGCAGGCCGGATGGTGGGAATCGGTGTGG
  AGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGT
  CACACCAGGGCTTTATGCGATGGTGGGAGCTGCAGCCTGTCTAGGTGGGGTGACTAGGATGACAGTGTCT
  CTAGTGGTCATTATGTTTGAACTGACTGGAGGTCTGGAGTATATTGTACCCCTGATGGCAGCTGCTGTCA
  CCAGCAAGTGGGTGGCTGATGCCTTTGGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTA
  CCCATTTCTTGATGTGAAGGATGAGTTCACCCACCGTACGCTGGCCACTGATGTGATGCGGCCCCGGAGG
  GGAGAACCGCCATTATCGGTACTAACCCAGGACAGCATGACTGTGGAGGACGTGGAGACTCTCATCAAGG
  AGACAGACTACAACGGCTTTCCTGTGCTCGTCTCCAGAGACTCGGAGCGTCTCATCGGGTTTGCCCAGAG
  GCGGGAGCTAATCTTGGCTATAAAAAATGCCAGGCAGAGGCAAGAGGGCATTGTGAGCAATTCCATCATG
  TACTTCACAGAGGAGCCTCCTGAGCTGCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTTTGA
  ACCTGAGCCCTTTCACGGTCACAGATCACACCCCCATGGAGACGGTGGTGGACATTTTCCGGAAACTGGG
  GCTCCGACAATGCCTGGTGACACGGAGTGGGAGACTTCTTGGGATCATCACAAAAAAGGATGTTCTGAGA
  CACATGGCCCAGATGGCAAACCAGGACCCTGAATCCATCATGTTTAATTAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACA
  AGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: BC005553
• Insert Size: 2151 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005553, AAH05553
• RefSeq Size: 2447 bp
• RefSeq ORF: 2150 bp
• Locus ID: 12727
• Cytogenetics: 7 4.23 cM
• Gene Summary: Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).[UniProtKB/Swiss-Prot Function]