Nolc1 (NM_001039351) Mouse Untagged Clone

CAT#: MC220591

Nolc1 (untagged) - Mouse nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nolc1
• Synonyms: NOPP130; NOPP140; P130
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220591 representing NM_001039351
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATACCGGCTTGCGCCGCGTGGTTCCCAGCGACCTTTATCCCCTTGTGCTCAGATTTCTGCGGG
  ATAGCCAACTCTCGGAGGTGGCCAGTAAATTTGCAAAAGCGACCGGCGCTACACAGCAGGACGCCAATGC
  CTCGTCCCTCTTGGACATCTATAGCTTCTGGCTCAAGTCCACCAAAGCCCCAAAGGTGAAGTTACAGTCA
  AATGGACCAGTGACCAAGAAGGCTAAGAAAGAGACTTCATCCAGTGACAGCAGTGAGGACAGCAGTGAGG
  ACGAGGACAAAAAAGCCCAGGGACTTCCCACACAGAAGGCTGCCGCACAGGTCAAGCGAGCCAGTGTGCC
  TCAGCATGCTGGAAAGGCAGCAGCCAAAGCTTCAGAGAGCAGCAGTAGTGAAGAATCCAGTGAGGAAGAG
  GAAGAGGACAAAAAGAAAAAGCCTGTCCAGAAGGCAGCTAAGCCCCAAGCCAAGGCAGTCAGACCTCCTG
  CGAAGAAGGCAGAGAGCTCTGAGTCGGACTCAGACTCGGATTCGGACTCCAGCTCAGAGGAAGAAACACC
  ACAGACCCAGAAGCCAAAGGCAGCTGTGGCAGCAAAAGCTCAGACTAAAGCCGAAGCCAAACCAGGTACA
  CCAGCGAAAGCACAGCCTAAGGTAGCCAATGGCAAAGCAGCCGCCAGCAGCAGCAGCAGCAGCAGCAGCG
  ATGACTCAGAGGAAGAGAAGAAGGCAGCTGCACCTCCCAAGAAGACTGTACCAAAAAAGCAAGTCGTGGC
  CAAGGCCCCAGTGAAAGTAGCTGCCGCCCCCACCCAGAAGAGCTCCAGCAGTGAGGATTCTTCCAGTGAA
  GAGGAGGAGGGACAGAGACAACCCATGAAGAAAAAAGCAGGTCCCTACAGTTCAGTTCCACCACCCTCTG
  TTCCTTTACCAAAGAAGTCCCCGGGAACCCAGGCTCCAAAGAAAGCTGCTGCGCAGACACAGCCTGCAGA
  CAGCAGTGACGACAGCAGTGACGATTCTGATTCAAGTTCTGAGGAAGAGAAAAAACCTCCAGCTAAGACG
  GTCGTCTCCAAGACACCCGCCAAAGCAGCTCCAGTGAAGAAGAAAGCAGAAAGCTCTTCAGACAGCTCGG
  ATTCTGACAGTTCTGAGGATGAAGCTCCTGCCAAGCCAGTCAGTACAACCAAGAGTCCCAAGCCAGCTGT
  CACTCCGAAGCCATCTGCAGCAAAGGCAGTGACAACTCCTAAGCAACCTGCAGGCAGTAACCAGAAACCT
  CAGAGCAGGAAGGCTGACAGCAGCTCCAGCGAGGAGGAAAGCAGCTCCAGCGAGGAGGAGGAGGCCTCCA
  AGAAAAGTGCCACAACCCCCAAGGCCAAGGTGACTGCTAAAGCAGCACCCGCCAAACAGGCCCCTCAGGC
  TGCTGGGGACAGCAGCTCTGACTCAGATAGTTCCAGCAGTGAAGAGGAGGAGAAGACTCCTAAGCCCCCA
  GCTAAGAAGAAGGCAGCAGGTGGAGCCGTTTCTACACCAGCCCCTGGGAAGAAAGCAGAGGCCAAGAGCA
  GCAGCAGCAGCAGCAGCAGCAGCTCCGAAGATTCCAGTGAAGAGGAGAAAAAAAAGAAGCCCAAAGCTAC
  TACCCCTAAAATACAGGCAAGCAAGGCCAATGGCACTCCAGCTTCTCTGAATGGAAAAGCAGCCAAGGAA
  AGTGAGGAGGAAGAGGAGGAGGAAGAAACAGAAGAGAAGAAAAAGGCAGCTGGGACCAAGCCAGGTTCAG
  GCAAAAAACGGAAGCAGAATGAGACCGCAGATGAAGCAACAACTCCTCAAGCTAAGAAAGTTAAGCTCGA
  GACCCCCAATACGTTTCCAAAAAGGAAGAAGGGAGAAAGAAGGGCGTCTTCCCCTTTCCGAAGGGTCAGG
  GAGGAGGAGATTGAGGTGGACTCTCGAGTGGCGGACAATTCCTTTGATGCCAAGCGAGGTGCAGCTGGAG
  ACTGGGGGGAGCGAGCCAATCAGGTTCTGAAGTTCACCAAAGGCAAGTCCTTCCGGCATGAAAAAACGAA
  GAAGAAGCGAGGCAGCTACCGGGGAGGCTCCATCTCTGTCCAGGTCAATTCCGTCAAATTCGACAGCGAG
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039351
• Insert Size: 2103 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039351.2, NP_001034440.1
• RefSeq Size: 3659 bp
• RefSeq ORF: 2103 bp
• Locus ID: 70769
• UniProt ID: E9Q5C9
• Cytogenetics: 19 C3
• Gene Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (By similarity). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (By similarity). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:11424213). It has intrinsic GTPase and ATPase activities (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the central coding region, compared to variant 3. The encoded isoform (B) is shorter, compared to isoform C.