Mdm1 (NM_010785) Mouse Untagged Clone

CAT#: MC220495

Mdm1 (untagged) - Mouse transformed mouse 3T3 cell double minute 1 (Mdm1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdm1
• Synonyms: Arrd2; Mdm-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220495 representing NM_010785
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGCGCTTCAAGGGGCTGAGTGAATACCAGAGAAACTTCCTGTGGAAAAAGTCCTATTTGTCAG
  AGTCTTATAATCCCTCAGTGGGACAAAAGTACTCATGGGCAGGACTTAGATCGGATCAGTTGGGGATCAC
  GAAAGAACCAGGTTTTATTTCAAAAAGAAGAGTTCCCTACCATGACCCTCAGATTTCAAAATACCTGGAG
  TGGAACGGAACCGTCAGAAAGAAGGATACGCTTGTCCCACCAGAACCCCAGGCCTTTGGAACGCCAAAGC
  CACAAGAGGCTGAGCAAGGAGAAGATGCCAATCAAGAAGCAGTTCTCTCACTAGAGGCCTCCAGGGTTCC
  CAAGAGAACTCGGTCTCATTCTGCGGACTCGAGAGCTGAAGGGGTTTCAGACACTGTGGAAAAGCACCAG
  GGTGTCACGAGAAGCCATGCGCCAGTTAGCGCGGATGTGGAGCTGAGACCTTCCAGCAAACAACCTCTCT
  CCCAGAGCATAGATCCCAGGTTGGATAGGCATCTTCGTAAGAAAGCTGGATTGGCCGTTGTTCCCACGAA
  TAATGCCTTGAGAAATTCTGAATACCAAAGGCAGTTTGTTTGGAAGACTTCTAAAGAAAGCGCTCCAGTG
  TTTGCATCCAATCAGGTTTTCCGTAATAAAAGCCAAATTATTCCACAGTTCCAAGGCAATACATTCACCC
  ACGAGACTGAATACAAGCGAAATTTCAAGGGTTTAACTCCAGTGAAGGAACCAAAGTCAAGAGAGTATTT
  GAAAGGAAACAGCAGTCTGGAGATGCTGACTCCAGTAAAGAAGGCAGATGAGCCTTTAGACTTAGAAGTA
  GACATGGCGTCGGAAGACTCAGACCAGTCTGTAAAGAAGCCTGCTTCATGGAGACACCAAAGGCTTGGAA
  AAGTGAATTCTGAATATAGAGCAAAGTTCCTGAGCCCAGCCCAGTATTTCTATAAAGCTGGAGCTTGGAC
  CCGGGTGAAGGAGAACCTGTCAAACCAGGTTAAGGAGCTCCGAGAAAAGGCCGAATCTTACAGGAAGCGA
  GTTCAGGGGACACATTTTTCTCGGGACCATCTGAACCAGATTATGTCGGACAGCAACTGCTGTTGGGACG
  TCTCCTCAGTCACAAGCTCGGAAGGCACCGTCAGTAGCAACATCCGAGCACTGGATCTTGCTGGAGACCT
  TACAAACCACAGGACCCCCCAGAAACACCCTCCTACCAAACTAGAAGAAAGAAAAGTTGCCTCGGGAGAG
  CAGCCCCTGAAAAACTCCACCAGGAGACTGGAGATGCCAGAGCCTGCCGCCTCGGTCAGGAGGAAGCTGG
  CTTGGGATGCTGAGGAGAGCACGAAGGAAGACACCCAGGAGGAGCCCAGGGCGGAGGAGGACGGGAGAGA
  GGAGAGAGGACAGGACAAGCAGACCTGTGCGGTAGAGCTGGAGAAACCGGACACACAGACACCCAAGGCA
  GACAGACTGACAGAAGGGTCGGAGACATCTTCTGTTTCCTCAGGGAAGGGAGGCAGGCTTCCTACACCGA
  GGCTGAGAGAACTCGGTATCCAGCGGACGCACCATGATCTCACGACGCCAGCTGTTGGTGGCGCAGTCTT
  AGTGTCTCCATCTAAAGTGAAGCCACCAGGCCTCGAGCAGAGGAGGAGAGCGTCCTCCCAAGATGGCTTA
  GAAACTCTGAAGAAAGACATTACTAAGAAAGGAAAACCCCGTCCCATGTCTCTGTTGACTTCTCCGGCTG
  CTGGCATGAAGACAGTTGATCCCCTGCCTCTGCGAGAAGACTGTGAAGCCAATGTGCTCAGATTTGCTGA
  TACTCTTCCTGTTTCGAAAATTTTGGACCGTCAGCCCAGCACCCCTGGGCAGCTGCCTCCATGTGCCCCG
  CCTTACTGTCATCCGTCCAGCAGGATCCAGGGCCGTCTGCGAGACCCTGAGTTTCAGCACAACAATGCAG
  ATAGACTGTCTGAGATCTCTGCTCGCTCTGCAGTTTCCAGCCTCCGGGCTTTCCAGACTCTAGCCCGAGC
  TCAGAAAAGAAAGGAGAATTTCTGGGGCAAGCCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010785
• Insert Size: 2067 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010785.2, NP_034915.2
• RefSeq Size: 3071 bp
• RefSeq ORF: 2067 bp
• Locus ID: 17245
• UniProt ID: Q9D067
• Cytogenetics: 10 66.65 cM
• Gene Summary: Microtubule-binding protein that negatively regulates centriole duplication. Binds to and stabilizes microtubules.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.