Acss1 (NM_080575) Mouse Untagged Clone

CAT#: MC220439

Acss1 (untagged) - Mouse acyl-CoA synthetase short-chain family member 1 (Acss1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acss1
• Synonyms: 1110032O15Rik; Acas2; Acas2l; AceCS2; AI788978
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220439 representing NM_080575
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCGCAGCCTCGGCAGCGGTGTGGGGCGACTGCTGCGTGGCCTGCAAGGGCGCTCTGGACAAT
  CGGGGTGGTCACTGAGCGTGTCCCGCTCGACCGCCACCCGACTCCCGGGCTGCGTTCCTGCAGCAGCGCA
  ACCAGGCTCGTACCCCGCGTTGAGCGCTCAGGCAGCCCAGGAGCCGGCCGCCTTCTGGGGGCCGCTGGCC
  CGTGACACACTAGTTTGGGACACTCCTTACCATACTGTCTGGGACTGTGACTTCAGGACGGGCAAGATCG
  GCTGGTTCCTGGGAGGCCAGTTGAATGTGTCTGTCAACTGCCTGGATCAGCATGTGCAGAAGTCTCCTGA
  GACCATAGCTTTGATCTGGGAGAGAGATGAACCTGGGACAGAAGTGAGAATCACTTACAGGGAGCTTCTG
  GAGACCACATGCCGCCTGGCCAATACACTGAAGAGACATGGAGTGCACCGAGGGGACCGTGTGGCCATCT
  ACATGCCTGTGTCCCCACTGGCTGTGGCAGCAATGCTGGCCTGTGCCAGGATTGGAGCTATCCACACAGT
  GGTATTTGCTGGCTTTAGTGCAGAGTCCTTGGCTGGGAGGATCAATGATGCCAAATGCAAGGCTGTTATC
  ACCTTCAACCAAGGACTCAGGGGAGGGCGTGTGGTGGAGCTGAAGAAAATAGTGGATGAAGCTGTGAAGA
  GCTGCCCAACAGTCCAGCATGTCCTGGTGGCTCACAGGACGGACACCAAGGTTCCCATGGGGAGTCTGGA
  TATCCCCCTTGAACAGGAGATGGCCAAGGAGGCCCCTGTTTGCACCCCTGAGAGCATGAGCAGTGAAGAC
  ATGCTCTTTATGCTCTACACCTCAGGGAGCACCGGGACACCCAAGGGACTCGTTCATACACAGGCAGGCT
  ATCTACTGTATGCCGCCATGACGCACAAGCTCGTGTTTGACTACCAGCCAGGTGATGTCTTTGGCTGTGT
  GGCTGACATCGGTTGGATCACAGGACACAGCTATGTGGTGTATGGACCCCTCTGCAATGGAGCTACCACA
  GTCCTTTTTGAGAGCACCCCAGTTTACCCTGATGCTGGTCGTTACTGGGAGACAGTGCAGAGGCTAAAGA
  TCAACCAGTTCTATGGTGCCCCGACAGCTGTCCGGCTGCTGCTGAAGTATGGGGATGCCTGGGTGAAAAA
  GTATGACCGCTCTTCCCTGCGCACACTGGGGTCAGTGGGAGAGCCTATCAACCACGAAGCCTGGGAGTGG
  CTCCACAAAGTCGTGGGTGATGGCAGATGTACACTGGTGGACACTTGGTGGCAAACGGAAACTGGAGGCA
  TCTGCATTGCACCACGGCCCTCGGAAGATGGGGCAGAGATCCTCCCGGGCATGGCCATGAGGCCGTTTTT
  TGGCATCGTTCCTGTACTCATGGATGAGAAGGGCAATGTTTTGGAGGGTGGAGATGTCTCTGGGGCCTTG
  TGTATTTCCCAAGCTTGGCCAGGCATGGCAAGGACCATCTATGGTGACCACCAGAGGTTCGTAGATGCCT
  ACTTCAGAGCGTACCCAGGTTATTACTTCACTGGAGACGGAGCTCACCGGACAGAGGGTGGCTATTACCA
  GATCACGGGGCGCATGGATGATGTCATCAATATCAGTGGTCATCGCCTGGGGACTGCAGAGATTGAGGAT
  GCAATGGCTGACCATCCCGCTGTTCCAGAGACTGCTGTCATTGGGTACCCTCATGATATCAAAGGAGAAG
  CTGCATTTGCCTTCATTGTGCTGAAAGATAACATCAGTGATGAGAACATGGTAGTGAATGAACTCAAATT
  GTCGGTGGCCACCAAGATCGCCAAGTATGCTGTGCCTGACCAGATCCTGGTGGTGAAGCGTCTCCCCAAA
  ACCAGATCTGGGAAAGTGATGAGGAGACTACTGAGGAAGATCATCACCAGCAGGGGACAGGATCTAGGGG
  ACACCACTACCCTGGAGGACCCCAGCGTCATCACAGAAATCTTGAGTGCCTTCCAGAAGTATGAAGAGCA
  GCGGGCTGCTACCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080575
• Insert Size: 2049 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080575.2, NP_542142.1
• RefSeq Size: 3594 bp
• RefSeq ORF: 2049 bp
• Locus ID: 68738
• UniProt ID: Q99NB1
• Cytogenetics: 2 G3
• Gene Summary: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).[UniProtKB/Swiss-Prot Function]