Ttll10 (NM_029264) Mouse Untagged Clone

CAT#: MC220388

Ttll10 (untagged) - Mouse tubulin tyrosine ligase-like family, member 10 (Ttll10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttll10
• Synonyms: 4833412E22Rik; 4930595O22Rik; AU022799; BC042775; Ttll5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220388 representing NM_029264
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTACACCCACAGGCCGGAAGGCCTCACAGAGATGGAAGGCTGGGACATCGGGCTGCTCGAAGAC
  CCCGAAGGGGTATAGGGACTACATCTGCCTCAAGGGTTCCTCGCCCTGGTGCCCTTATGCCTGCAACACG
  AAACCGTCCAAGATTCATCCACTGCCGGGGTCAGCCCCCTCGAACCCGTGTCAGCTCCAAGAGATCCAAA
  CGGTCCAGAATCCATCCGTGCCACACTGAGGTGCCAGGCTGGACCCATGAGAAACAGATGGGGAGCAGCG
  TCAAGGAAAGGCTCCGGCCTGAGCTAAGTCAGCTGGACCAAGATGCAGACGACCTGGAGGAAGAAGAAGC
  TGCCAGACTCCCAGTGACCTCCCCGGACGGGCTCCTGATGGAAGGAGACAAGCAGCCCAGTCCCGGTCAG
  GGCCCTTTCTTCTACATTGGAGGCACCAACGGAGCATCAATAATCAGCAACTACTGTGAGAGCAAGGGCT
  GGCAACGCACTCAGGACAGCCACTGTGAGGACTACAAGCTAAAGTGGTGTGAGATCAAGTGCAGGGACAA
  CTACTGCAGCTTTCGGGAAGGCCAGCAGTTGCTGTTCCAGCTCCCCAACAACAAGCTTCTTACGACCAAG
  ATCGGGTTGCTCAGTGCTCTGCGGGAGCACGCACGCACTTTGAGCAAAGCCAGGATGCTGCCCAGTACCC
  AAACCAAGGTCCTGAAAATGGAAGAGTTTTTTCCAGAGACCTATCGGCTGGACATCAGAGACGAGAGGCA
  GGCTTTCTTCGCTCTATTTGATGAAACCCAGATGTGGATCTGCAAACCTACGGCCTCCAACCAGGGAAAG
  GGCATCTTTCTGATCAGGAGCCAGGAGGAGGCTGCTGCCCTGCAGGCCAAGACCCAGAGCATTGAGGATG
  ACCCCATCTACCGCAAGATGCCCTTCCGTGCACCACAGGCGCGAGTGGTGCAGAGATATGTCCAGAACCC
  GCTGCTGCTAGATGGGAAGAAGTTTGATGTGCGCTCCTACATGCTCATTGCCTGTGCCATGCCTTACATG
  GTCTTTTTTGGCCATGGCTATGCCCGCCTCACCCTCAGCCTGTATAACCCTCATTCCAGTGACCTCAGTG
  GTCATTTGACCAATCAGTTCATGCAGAAGAAGAGCCCCCTGTACACGCTGCTGAAGGAGAGCACCGTGTG
  GACCATGGAGCACCTCAACCGATACATCAATGACAAATTTAGGAAGACCAAGGGCCTCCCCAGAGATTGG
  GTCTTCACCACCTTCACGAAGCGGATGCAGCAGATCATGTCCCACTGCTTCCTGGCTGTCAAGTCCAAGC
  TGGAGTGCAAGCTGGGCTACTTTGACCTCATTGGCTGTGACTTCCTGATTGATGAAAACTTCAAGGTGTG
  GCTGCTGGAGATGAACGCCAACCCTGCCCTGCATACCAACTGTGAAGTCCTGAAGGCAGTGATCCCCGGA
  GTGGTTATAGAGACCTTGGACCTGGCGCTTGAGACCTGCCAAAAGAGCTTGCACAGTCAGAAGATGCTGC
  CTCTGCTGTCACAGCGTCGCTTTGTGCTCCTTTACAACGGGGAGACTACGGACTTGTGGCCGCGCTTGGC
  GAGCTCGCGCCCACTTAACCGCTTGCCTAACCCTCACCCTAACCCTAACCCTAACCCTCACCCTCACCCT
  CACCCTCACCCTAACCCTAACCCTCACCCTCACCCTCACCCTAACGCTAACCCTCACCCTCCCCGGCCCA
  CCTGTGAAGCCGCATCCTCTGCGCTTTCCTCCGCACGGGCCGCTATCTCAGAACGGCCTGGTGCACGCAA
  GTCGATGCCCTCCAGGGGAGCTCCCGTGTGCACACCCCGCAAATCGCGGCTGTCTGACTCGAGCGGTAGC
  TCCATAGCGGAGTCTGAGCCCTCTCTCTGTTCTGGGTCCCTGGAAGGCAGCAGGGACACAGCCCGGGAAC
  CCTCCCTGGGTCCGCCCGAGGAGGAGCGCGAGGAGGAACAGAGAAGTACGAGCCATCGAGGCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029264
• Insert Size: 2028 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029264.2, NP_083540.1
• RefSeq Size: 2307 bp
• RefSeq ORF: 2028 bp
• Locus ID: 330010
• UniProt ID: A4Q9F3
• Cytogenetics: 4 E2
• Gene Summary: Polyglycylase which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues of target proteins. Polyglycylates alpha-tubulin and beta-tubulin, but is not able to initiate glycylation and only has activity toward monoglycylated tubulin. Has the ability to polyglycylate non-tubulin proteins such as NAP1; in this case it can initiate glycylation and does not require preliminary monoglycylation by another glycylase.[UniProtKB/Swiss-Prot Function]