Gpsm1 (NM_153410) Mouse Untagged Clone

CAT#: MC220348

Gpsm1 (untagged) - Mouse G-protein signalling modulator 1 (AGS3-like, C. elegans) (Gpsm1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpsm1
• Synonyms: 1810037C22Rik; Ags3; AW107933
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220348 representing NM_153410
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCCCGGCCCCGCCCGTGGCCGAGGAGCTCCCGGGCCCGGCCTCCAGGCGCCTCTACTCCAGGA
  TGGAGGCCTCCTGTCTAGAGCTGGCCCTGGAAGGGGAGCGGCTGTGCAAGGCGGGGGACTTCAAGGCAGG
  TGTGGCTTTCTTCGAGGCTGCTGTGCAGGTGGGCACTGAGGACCTGAAGACACTGAGTGCCATCTACAGC
  CAGCTGGGCAATGCCTACTTCTACCTGAAGGAGTATGCCCGGGCCCTGCAGTTCCACAAACATGACTTGC
  TGCTGGCACGGACCATTGGTGACCGCATGGGGGAGGCCAAGGCTAGTGGGAACCTGGGCAACACACTCAA
  GGTCCTAGGCCGATTCGATGAGGCAATCGTCTGCTGCCAACGACACTTGGACATTGCCCAGGAGCAGGGG
  GACAAGGTTGGGGAGGCGAGAGCACTCTACAACATTGGAAATGTGTACCACGCCAAGGGCAAACAGCTTT
  CCTGGAATGCTGCACAGGACCCCGGGCACCTGCCACCTGATGTCCGCGAGACACTGCACAGGGCCTCTGA
  GTTTTATGAGAGGAACCTGTCTTTGGTGAAGGAACTAGGCGACCGGGCGGCCCAGGGCAGGGCCTATGGC
  AACCTGGGTAACACCCACTACCTACTGGGAAACTTCACGGAGGCCACAACCTTCCACAAAGAGCGCCTGG
  CCATCGCCAAGGAGTTTGGGGACAAGGCAGCTGAGCGGAGGGCCTACAGCAACCTGGGCAATGCTCACAT
  CTTCTTGGGGCGCTTTGATGTGGCTGCTGAACATTACAAGAAGACGCTGCAGCTGTCTCGGCAGCTCCGG
  GACCAGGCAGTGGAGGCTCAGGCTTGCTACAGCCTGGGCAACACCTACACACTGCTACAGGACTACGAGC
  GTGCTGCTGAGTACCACCTGCGGCACCTAGTCATTGCCCAGGAGCTGGCTGATAGGGTGGGAGAGGGCCG
  AGCATGCTGGAGCCTGGGGAACGCCTATGTGTCCATGGGGAGCCCTGCACAGGCCTTGACCTTTGCCAAG
  AAACATCTGCAGATCTCCCAGGAGATTGGAGACCGAAACGGAGAACTGACAGCCCGCATGAATATTGCCC
  ACCTGCAGCTGGCCCTGGGCCGGCTGACTAGCCCAGCAGCAGCGGAGAAGCCAGATCTGGCTGGCTATGA
  GGCACAAGGAGCAAGACCCAAACGGACACAGAGGCTGAGTGCCGAGACCTGGGACCTGCTGCGGCTCCCC
  CTGGACCGGGAGCAGAATGGAGAGACCCACCACACAGGGGACTGGAGGGGTCCAGGCAGGGACTCGCTTC
  CCCTCCCCATGAGGAGCAGGAAGTACCAGGAAGGTCCAGATGCCATTGAGAGGAGACCCCGGGAGGGTAG
  TCACTCTCCACTGGACAGTGCTGATGTAAGGGTACAGGTGCCTCGTACGGGTATTCCCAGGGCCCCGTCT
  TCTGACGAGGAGTGTTTCTTTGACCTGCTGAGTAAGTTCCAGAGCAGTCGCATGGATGACCAGCGCTGTC
  CCCTGGAGGAAGGCCAGGCTGGGGCTGCTGAGGCCACAGCTGCCCCATCCGTGGAGGATAGAGCAGCTCA
  GTCCTCCGTGACAGCTTCACCACAGACAGAGGAGTTCTTTGACCTCATTGCCAGCTCCCAGAGCCGCCGG
  CTGGACGACCAGAGGGCTAGCGTAGGCAGCCTGCCTGGGCTACGCATCACCCTCAACAATGTGGGGCACC
  TCCGAGGCGACGGGGACGCCCAGGAGCCGGGGGATGAGTTTTTCAACATGCTTATCAAATACCAGTCCTC
  CAGGATTGATGACCAGCGCTGCCCACCCCCTGATGTGCTGCCCCGTGGCCCTACCATGCCTGATGAGGAT
  TTCTTCAGCCTTATCCAGAGGGTGCAGGCTAAGCGGATGGATGAGCAGCGTGTGGACCTTGCTGGGAGTC
  CAGAGCAAGAGGCCAGTGGGCTGCCTGATCCCCAGCAGCAGTGTCCACCGGGTGCCAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153410
• Insert Size: 2022 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153410.5, NP_700459.2
• RefSeq Size: 3389 bp
• RefSeq ORF: 2022 bp
• Locus ID: 67839
• UniProt ID: Q6IR34
• Cytogenetics: 2 A3
• Gene Summary: Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) has an alternate 5' terminus which results in the use of an alternate tranlation initiation site, compared to variant 3. Variant 1 encodes isoform 1 which has a shorter and distinct N-terminus, compared to isoform 3.