Phactr4 (NM_001161797) Mouse Untagged Clone

CAT#: MC220277

Phactr4 (untagged) - Mouse phosphatase and actin regulator 4 (Phactr4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phactr4
• Synonyms: 3110001B12Rik; AI527228; AW495572; C330013F19Rik; mKIAA4120; N28169
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220277 representing NM_001161797
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACCCATCGGAAGAAGCAGAGCAGCCCTCTGGAGACCCAGGCATGGGCATGGACAGTGTGGAAG
  CAGGGGACACAACACCTCCCACCAAAAGGAAAAGCAAGTTCTCAGCGTTAGGCAAGATCTTCAAGCCGTG
  GAAATGGAGGAAAAAAAAAAGCAGTGATAAGTTCAAAGAGACATCAGAAGATGGTGAGGATTCAGGAAAG
  CTGAGCCATGCTGCATTAAAGAACGGACACACCACCCCCATAGGCAGTGCCAGGTCCTCTAGCCCAGTCC
  TGGTAGAAGAAGAGCCAGAGAGAAGTCTCAGGAACCTCACTCCAGAAGAGGAGTCAAAGAAGAGACTTGG
  CTCAACTGGAAGCCAGCCTAACTCTGAAGCCGAGCCTGGTCCTGAGCATGCACCTAAGCAGCCTCTGCTG
  CCCCCCAAGAGACCCTTGTCCTCTTCTTGTGAAGCAAAGGAGGTTCCGGCTGGCAGTACAGCAAGGTCCG
  TCTCCTCCACCTCTGGCTCTACCACCGTGACATCTGCTGCCACCACTGCTGCCACAGACATGACAAAGAC
  TGTTAAGTCCTTTGTGGGCCCCACCCCCGCTCCTGCCCCCGCCCCCAGGACTCTGCCTGCTGCTCCTGCC
  AGTGCTAACACTGCTGCCACCACTACAGCCCCTGCCAAGCAACCCCCCATCCCACCCCCTAAACCAGCTC
  AGAGAAACAGCAACCCCATCATTGCTGAACTATCCCAAGCAATGAACAGTGGCACAGTGCTGTCGAAACC
  ATCCCCACCCTTGCCACCCAAGAGAGGCATTCCATCAACTTCGATACCCAGCTTGGAGCCTGCTGCTTCG
  TTCACAACTAAGACAGCAAATGATCAAAGAGAAAAAACTGTCTCTTTGTGTTTGGAACCACCACTGATAA
  TCCCGCCTTCGTCCCCATCACCCCCACTGCCTACCCACATACCTCCCGAGCCTCCACGGTCCCCTCTAGT
  GCCTGCTAAGACTTTTCAAATTGTGCCAGAGGTTGAGTTTTCCTCTTCTTCAGATCTATTCCAGGACATT
  TCCCAGCAGGAGGATCAGAAAACAGAAGTCCCCAAGAAGATACAGGATCAGAGCTTTGGGGAGTCCCATA
  TACCCTCCAGGCTGCCCCCACTCCCACTGCATATCCGAATTCAGCAGGCCCTTACCAGCCCCCTTCCTGT
  GACCCCTCCCTTGGAAGGCACTCACAGAGCCCATTCACTGCTTTTTGAGAACAGTGACAGCTTCTCCGAG
  GACACTGGTACCCTGGGTCGTACCAGGTCACTTCCCATCACGATTGAGATGTTGAAAGTTCCAGATGATG
  AAGAGGAAGAGCAGACCTGCCCGTTTGTTGAAGACGTGACGTCTACCTCAGCCACTCCTAGCCTACCACT
  GTGTCTGCGAGAGGAAGAGAAGGAGAGTGACTCAGATTCAGAGGGTCCCATTAAGTACCGAGATGAAGAA
  GAAGATGATGATGATGATGAAAGCCATCAGAGTGCCCTGGCCAACAGAGTGAAGAGGAAGGACACGCTGG
  CAATGAAGCTGAGCAGCAGACCCAGTGAACCAGAGACCAACCTGAATTCTTGGCCTCGTAAAAGCAAGGA
  GGAGTGGAATGAAATACGGCACCAGATCGGGAACACGCTGATCCGGCGGCTGAGTCAGAGACCAACAGCA
  GAAGAATTGGAGCAGCGGAACATACTACAGCCTAAAAATGAAGCTGATCGGCAAGCAGAGAAACGGGAAA
  TCAAGCGTCGCCTCACTAGAAAGCTCAGTCAAAGGCCAACTGTGGCTGAACTGCTTGCCAGGAAGATTCT
  GCGGTTTAATGAGTATGTGGAAGTAACTGATGCTCATGACTATGACCGGCGAGCAGACAAACCATGGACC
  AAACTGACTCCTGCTGACAAGGCTGCCATAAGGAAAGAATTAAATGAATTTAAGAGCTCGGAGATGGAGG
  TTCATGTAGACAGCAAACACTTTACACGCTACCATCGCCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161797
• Insert Size: 2004 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161797.1, NP_001155269.1
• RefSeq Size: 4504 bp
• RefSeq ORF: 2004 bp
• Locus ID: 100169
• UniProt ID: Q501J7
• Cytogenetics: 4 D2.3
• Gene Summary: Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter isoform (2) compared to isoform 1.