5730559C18Rik (NM_028872) Mouse Untagged Clone

CAT#: MC220258

5730559C18Rik (untagged) - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 5730559C18Rik
• Synonyms: 1700034M08Rik; 4933426C09Rik; AI586180; D1Mgi54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220258 representing NM_028872
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCAGGGGACAGATGTCTTGGGATCTTTAACATTTGGGATGCTGCAAATGCCGAAGTTAAATGAAA
  TACCTCCGGGGAGGGGAGGCCCGGGGGAGCCTTGGGGAGAGGGAAGATGGGCGGGACCAACAGGTCCTGA
  AGCTGCGCGACCGGCGCGGGGGGCGCGGGGGCAGGCGCGAGGCGCCAGAGCTCGGTGGGACAGCTGGGAA
  CACTCCAGACTACCTACACATCCTGGCCCAGGCTGGGACCAGTGCAGCCCCTCCTTCCTCTGTGCCCCCT
  CTTCCCAGAAACTCATCATGGAGAGTAAGGATGAAGTCAGTGACTCCGACAGCGGCATCATCCTGCAGTC
  TGGCCCGGACAGCCCTGTCTCTCCAATGAAAGAGCTGACCAATGCAGTACGCAAGCAGCAGAGGGCTCTG
  GAAGCGAGGCTGGAGGCCTGCCTGGAGGAGCTGCGCAGACTCTGCCTGAGGGAGGCGGAGCTGACTGGGA
  CTTTGCCGGCAGAGTATCCCCTTAAGCCTGGTGAGAAGGCCCCCAAGGTCCGGCGCAGGATCGGAGCAGC
  GTACAAGCTGGATGAGTGGGCCCTCCACAGAGAGGACCCCCTGAGCAGCCTAGAGCGCCAGTTGGCTCTG
  CAGCTTCAGATCACCGAGGCGGCTCGAAGGCTGTGTGCAGAGGAGAACCTCAGCAGACAGGCACGCAGGC
  AGCGGAAGCACGCTGCGCTGCAGGAGGAGAAGAAGCTGCGGGACCTTCAGCGTTGCCTGGGTGATCGGCG
  GCGGAACAGTGAGCCCCCTCCTACCACTGTGCCCTCCCTGGGCAGAGAACTCAGTGCCTCTGACGACAGC
  TCCCTGTCTGATGGGCTGCTCCTGGAGGAAGAAGACTCCCAAGCGCCAAAACCTCCCCCAGAGTCACCGG
  CACCACCTTCGCGGCCTCTCCCACCCCAGAGCCTTGAGGGCCTACAGCCAACAGGACCTGAGTCTGGCGG
  CCAGGAACGGGCTCCCATCCAAAACAGTCCCTGGAAGGAGACCAGCCTGGACCACCCCTATGAGAAGCCC
  AGGAAGTCTTCTGAGCTCAGTAGCGAGTCCAGCAGCCCGGCCACCACTCCTCAGGACCAGCCCAACCCTT
  CCAGCCTGTGGGTGCTGGATGCTGCCTCTTACCACGTGGTCCCCATCCGAAATGTTCCCGGCCAGCGTCA
  GGGCCGCACCAGTGCCCCAGCCACCCCTGAGATGCAGGGCAGGAGGGGCCAGTCACAGTCTCTGAGGGTG
  GACTCCTTCCGAGCAGGCGCGGAGGGCCGAGGTCGCAGTGCCTTTCCCCGCCGCCGCCCCACTCATTACA
  CAGTGACAGTGCCAGACTCCTGCTTCACACCCGGCAAGCCCCCACTGCCACACCCTGCCTGCCATTCCTG
  CTCTGAAGACAGTGGCTCTGATGTCTCCAGCATCTCCCATCCCACCTCACCTGGCAGCAGCAGTCCAGAC
  ATCTCCTTTCTGAGGCCCCTCTGTCTCCCTGAGCCACCTCGCCACCGTGGGGCCTGGGGCCCAGCCTGTG
  GCAGAGAGCTGGCCCCTCATTACTCTAAACTTCTGCTACCCGCTGGATACTTCCCAACGGGGCGCTATGT
  GATGGTGGCCGAGGGCCACCTGCCGCCGGGCGAGTGGGAGCTGTGTCGTGCAGCCGTGGGTGCTGCCTAT
  GACGAGGAAGGTGCACCCCTGCGTTACCAGCGACTGGTACCCTCCCACAGCCGCATTGTGCGCACACCCT
  CACTAAAGGACAGTCCTGCGGGCCGGGGACTCAGCAAGGCAGCAGTCTCGGAGGAGCTCAAGTGGTGGCA
  TGAACGCGCACGTCTCCGGAGCAGCCGCCCCCACTCGCTCGACCGCCAAGGGGCGTTCCGTGTTAGGAGT
  CTGCCTCCTGGGAGGGAGAGCTTCGGGCGGGCCTCAGGACCCAGGACACAGGTGCCCCCCGTGTACGTAC
  TCCGGAGGTCGACGGACGGGGCCCCAGTGCAAGTCTTTGTGCCTGAGAACGGAGAGATTATCAGCCAAGT
  GTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028872
• Insert Size: 2034 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028872.3, NP_083148.3
• RefSeq Size: 3013 bp
• RefSeq ORF: 2034 bp
• Locus ID: 67313
• UniProt ID: Q7TN12
• Cytogenetics: 1
• Gene Summary: Expressed in peripheral macrophages and intestinal myeloid-derived cells, is required for optimal PRR (pattern recognition receptor)-induced signaling, cytokine secretion, and bacterial clearance. Upon stimulation of a broad range of PRRs (pattern recognition receptor) such as NOD2 or TLR2, TLR3, TLR4, TLR5, TLR7 and TLR9, associates with YWHAQ/14-3-3T, which in turn leads to the recruitment and activation of MAP kinases and NF-kappa-B signaling complexes that amplifies PRR-induced downstream signals and cytokine secretion (By similarity). In the intestine, regulates adherens junction stability by regulating the degradation of CYTH1 and CYTH2, probably acting as substrate cofactor for SCF E3 ubiquitin-protein ligase complexes. Stabilizes adherens junctions by limiting CYTH1-dependent ARF6 activation (PubMed:29420262).[UniProtKB/Swiss-Prot Function]