Dbf4 (NM_013726) Mouse Untagged Clone

CAT#: MC220248

Dbf4 (untagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dbf4
• Synonyms: AA545217; Ask
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220248 representing NM_013726
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCGAGACCATGAGGATCCACAGCAAAGCACCTCTCCCGGGTGGCATCCAAGACAGAAATGAAA
  AGAATCGACCATCCTTGAAATCCCTGAAGGCTGATAATAGACTGGAAAAATCCAAATATAAGCCCCTTTG
  GGGAAAAATATTTTACCTTGACTTGCCTTCTATCACCATATGTGAGAAACTACAAAAAGATATAAAGGAA
  CTCGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATTTTGTTTCAAATAAAAAGGAAGCAA
  AGTATGCACAGACATTGGGTCGCGTTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCAC
  CTCTCCTCATCCCAGCCATGATGGAAGTTCCTTTAAGTCTCAAGACAGAGTATGTTTGAGCCGAGGAAAA
  TTGTTAGCTGAAAAAGCTGTGAAGGATCATGATTTTATTCCTGCTAATAGTATATTATCAAATGCCTTAT
  CATGGGGAGTAAAGATTCTTCATATTGATGACATTCGATACTACATTGAACAGAAGAAGAAAGCACTGAG
  TGCACTCAAGAAATCCAGTGCTTCTGGGAAGGATGCGGGGAAAAAAGCTGGTCCTGGAATACAAAAGACA
  AGAACAGGGAGACTCAAGAAGCCTTTTTTAAAGGTTGAAGATGTGAACCGGTGCTACAGGCCATTTTACC
  TTCAGCTCCCCAGTCTGCCGTGTATAAACTACTTTCTTCAGAAGCCCTGCAGTCCGTTTGATATAGAGAA
  GTCATCCAGCGTCCAGAAACAAGCTCAGCCCAAGCTCAGAATTAATATGGACGGCGATAAATGTGGAACC
  CCAGTTCAACTCCAGTTGAAGGAAAAGAGAAAAAAAGGATATTGTGAATGTTGCTTACAGAAATACGAAG
  ATCTCGAAACTCACCTTCTGAGTGAGAAACACAGGAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGA
  TGATATTGTATCTCAGTTAGTTTTCGATTTTGTGGAATATGGAAGAGACACACCTCAAAAGAAAAGAATA
  AGATACAGTGTCGGGTCCCTTTCTTCTGTTTCTGCAAATGTCCTGAAAAACACTGCACCGAAGGAGAAGC
  CACTCTTGGAGCCCAATTTCCAGAAGGACGTGGGCGAAAGCAGTGGGCACCTGCTCAAGCCGAACTCTCA
  GTATGAAGAAACCCAGAAACCTGAAGAAAAGCATGGGTTTGCTTCAGAGCCCACAACCTATTCTTCAGCT
  GGATTGAAAGGATGTGACAGGAAACCAGTGAGCATGTTCAATGCAAGTGAGCCTGACCCAGAGCAGGAGT
  ATGCACAGCTACCTCTGCGTGACAGCACACCGGAACACCAAGTGACCGAGGGTAGAAATGACGGAGAACA
  GAGAGTAGACCCTGCTCCAGGCGTGTCGCAGTCATGTGGACAAGTTTCTCATCTCAGTACAGAAAGTAAT
  CTACCCCAACCACAGTTAGCTGCAGACATTACACAGCTTTCAGCAAAAGATCTGCAAGAAAAGGGCTTTC
  ATGTTGTGATAGGTCATGCTTCTGACCTGGTGGCATTAAATACATCAAAAGAGCAGCTAACAATGAAGGC
  GAGAACTCCACCCTGTAGTCCCCAGGAGCCTCATGAGTGTGACACTGAGAACATGGAGAATTTGCCTTGT
  GGTAAAATCCAGCGGAAAGTGAGAATGCTATTAGGGCAACAGAAAGCAAATGCTGAACCGAGTGCTGAAT
  TGGATAAAAAGAGAACTGAGTATCTTCCTGCACATGAAGACAGAACTTGTGGGTCACCAGTACAGTCTCT
  ATTGGACCTTTTTCAGACAAGCGAGGAGAAATCAGAATTTTTGGGTTTTACAGGCTACACTGAAAACAGT
  GGAATATGTGATGTTTTAGATATTTGGGAGGAAGAAAATTCAAGTACCCTGTTGTCAACGTTTTTTTCTT
  CCCCTTCAACTTCTGCATTCGTTGGATTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013726
• Insert Size: 1992 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013726.3, NP_038754.1
• RefSeq Size: 2444 bp
• RefSeq ORF: 1992 bp
• Locus ID: 27214
• UniProt ID: Q9QZ41
• Cytogenetics: 5 A1
• Gene Summary: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).