Bmx (NM_009759) Mouse Untagged Clone

CAT#: MC220149

Bmx (untagged) - Mouse BMX non-receptor tyrosine kinase (Bmx), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bmx
• Synonyms: Etk; Etk/Bmx; Tyro8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220149 representing NM_009759
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACAATATGGAGAGTAAATCCATTCTGGAAGAACTGCTTCTCAAAAAGTCACAGCAAAAGAAGA
  AAATGTCACCAAATAATTACAAGGAGCGGCTTTTTGTTCTAACCAAAACAAGCCTTTCCTACTATGAATA
  CGACAAAATGAAAAGAGGAAGCAGAAAAGGATCAATAGAAATTAAGAAAATCAGATGTGTGGAAAAAGTC
  AACCTTGAGGAGCAGACACCGGTGGAGAGACAGTACCCATTTCAGATTGTGTATAAAGATGGGCTTCTTT
  ATGTCTATGCATCGAATGAAGAGAGCCGATGTCAGTGGTTAAAAGCGCTGCAAAAAGAGATACGGGGCAA
  CCCTCACCTGTTGATCAAGTATCACAGTGGCTTCTTTGTGGATGGAAAGTTCCTGTGTTGCCAGCAGAGC
  TGCAAAGCAGCCCCAGGATGCACTCTCTGGGAAGCATATGCTGATCTGCACATCGCAATCAGTGATGAGA
  AACACAGAGCTCCCACTTTCCCAGAGAGGCTACTGAAGATTCCAAGGGCAGTTCCCGTTCTCAAAATGGA
  TGCATCATCTTCAGGTGCCATTCTACCCCAATATGACAGCTATTCAAAGAAAAGTTGTGGTTCCCAGCCA
  ACCAGCAACATACGCTATATTCCAAGGGAAGACTGCCCTGACTGGTGGCAAGTAAGAAAACTGAAAAGCG
  AGGAAGACATTGCATGCAGTAACCAACTGGAAAGAAATATCGCCTCTCACAGCACCTCAAAGATGTCATG
  GGGATTCCCTGAGTCAAGTTCATCAGAAGAAGAGGAAAATCTGCATGCTTATGACTGGTTTGCTGGGAAT
  ATCTCCAGGTCACAATCTGAGCAGTTACTGAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTCGGAATT
  CCAGCCAGATGGGAATGTACACTGTGTCCTTATTTAGTAAGGCTGTAAATGATAAAAAAGGAACTGTCAA
  GCATTACCACGTGCATACTAATGCTGAAAATAAATTATACCTGGCTGAAAACTACTGCTTTGATTCCATT
  CCAAAGCTCATTCACTATCACCAACATAATTCAGCAGGCATGATCACACGGCTCCGCCACCCAGTGTCAA
  CCAAGGCCAACAAGGTCCCGGTGTCTGTGGCTCTGGGAAGTGGAATTTGGGAACTGAAAAGAGAAGAGAT
  TACCTTGCTGAAGGAGCTGGGCAATGGCCAGTTTGGAGTGGTCCAGCTGGGCCAGTGGAAGGGGCAATAT
  GATGTGGCTGTAAAGATGATCAAGGAGGGTGCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACCA
  TGATGAAACTCAGCCATCCCAAGCTGGTGAAGTTCTACGGAGTATGCTCAAAGAAATACCCCATCTATAT
  AGTAACTGAGTATATAACAAATGGTTGCTTGCTTAATTACCTGAAGAGTCATGGGAAAGGACTAGAAAGT
  TGCCAGCTCTTAGAAATGTGTTATGATGTCTGTGAAGGCATGGCCTTCTTGGAGAGCCATCAGTTCATAC
  ATCGAGATTTGGCTGCTCGGAACTGTTTGGTGGACAGTGATCTCTCTGTGAAAGTCTCAGACTTTGGAAT
  GACGAGATATGTCCTTGATGATCAGTATGTCAGCTCAGTAGGAACCAAGTTTCCAGTCAAGTGGTCAGCT
  CCAGAGGTGTTTCACTACTTCAAATACAGCAGCAAGTCGGATGTATGGGCATTTGGGATCCTGATGTGGG
  AGGTGTTTAGTCTAGGGAAGCAGCCCTATGACTTATATGATAACTCCGAGGTGGTTGTGAAGGTCTCCCA
  GGGCCACAGACTCTACCGGCCCCAACTGGCATCAGACACCATCTACCAGATCATGTACAGCTGCTGGCAT
  GAGCTTCCAGAAAAGCGCCCCACATTTCAGCAACTCCTGTCTGCCATAGAACCACTTCGGGAACAAGATA
  AACCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009759
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009759.4, NP_033889.2
• RefSeq Size: 3005 bp
• RefSeq ORF: 1968 bp
• Locus ID: 12169
• UniProt ID: P97504
• Cytogenetics: X F5
• Gene Summary: Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.[UniProtKB/Swiss-Prot Function]