Ripk1 (NM_009068) Mouse Untagged Clone

CAT#: MC220144

Ripk1 (untagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ripk1
• Synonyms: D330015H01Rik; Rinp; RIP; RIP-1; Rip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220144 representing NM_009068
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACCAGACATGTCCTTGGACAATATTAAGATGGCATCCAGTGACCTGCTGGAGAAGACAGACCTAG
  ACAGCGGAGGCTTCGGGAAGGTGTCCTTGTGTTACCACAGAAGCCATGGATTTGTCATCCTGAAAAAAGT
  ATACACAGGGCCCAACCGCGCTGAGTACAATGAGGTTCTCTTGGAAGAGGGGAAGATGATGCACAGACTG
  AGACACAGTCGAGTGGTGAAGCTACTGGGCATCATCATAGAAGAAGGGAACTATTCGCTGGTGATGGAGT
  ACATGGAGAAGGGCAACCTGATGCACGTGCTAAAGACCCAGATAGATGTCCCACTTTCATTGAAAGGAAG
  GATAATCGTGGAGGCCATAGAAGGCATGTGCTACTTACATGACAAAGGTGTGATACACAAGGACCTGAAG
  CCTGAGAATATCCTCGTTGATCGTGACTTTCACATTAAGATAGCCGATCTTGGTGTGGCTTCCTTTAAGA
  CATGGAGCAAACTGACTAAGGAGAAAGACAACAAGCAGAAAGAAGTGAGCAGCACCACTAAGAAGAACAA
  TGGTGGTACCCTTTACTACATGGCACCCGAACACCTGAATGACATCAATGCAAAGCCCACGGAGAAGTCG
  GACGTGTACAGCTTTGGCATTGTCCTTTGGGCAATATTTGCAAAAAAGGAGCCCTATGAGAATGTCATCT
  GTACTGAGCAGTTCGTGATCTGCATAAAATCTGGGAACAGGCCAAATGTAGAGGAAATCCTTGAGTACTG
  TCCAAGGGAGATCATCAGCCTCATGGAGCGGTGCTGGCAGGCGATCCCAGAAGACAGGCCAACATTTCTT
  GGCATTGAAGAAGAATTTAGGCCTTTTTACTTAAGTCATTTTGAAGAATATGTAGAAGAGGATGTGGCAA
  GTTTAAAGAAAGAGTATCCAGATCAAAGCCCAGTGCTGCAGAGAATGTTTTCACTGCAGCATGACTGTGT
  ACCCTTACCTCCGAGCAGGTCAAATTCAGAACAACCTGGATCGCTGCACAGTTCCCAGGGGCTCCAGATG
  GGTCCTGTGGAGGAGTCCTGGTTTTCTTCCTCCCCAGAGTACCCACAGGACGAGAATGATCGCAGTGTGC
  AGGCTAAGCTGCAAGAGGAAGCCAGCTATCATGCTTTTGGAATATTTGCAGAGAAACAGACAAAACCGCA
  GCCAAGGCAGAATGAGGCTTACAACAGAGAGGAGGAAAGGAAACGAAGGGTCTCTCATGACCCCTTTGCA
  CAGCAGAGAGCTCGTGAGAATATTAAGAGTGCAGGAGCAAGAGGTCATTCTGATCCCAGCACAACGAGTC
  GTGGAATTGCAGTGCAACAGCTGTCATGGCCAGCCACCCAAACAGTTTGGAACAATGGATTGTATAATCA
  GCATGGATTTGGAACTACAGGTACAGGAGTTTGGTATCCGCCAAATCTAAGCCAAATGTATAGTACTTAT
  AAAACTCCAGTGCCAGAGACCAACATACCGGGAAGCACACCCACCATGCCATACTTCTCTGGGCCAGTAG
  CAGATGACCTCATAAAATATACTATATTCAATAGTTCTGGTATTCAGATTGGAAACCACAATTATATGGA
  TGTTGGACTGAATTCACAACCACCAAACAATACTTGCAAAGAAGAGTCGACTTCCAGACACCAAGCCATC
  TTTGATAACACCACTAGTCTGACTGATGAACACCTGAACCCTATCAGGGAAAACCTGGGAAGGCAGTGGA
  AAAACTGTGCCCGCAAGCTGGGCTTCACTGAGTCTCAGATCGATGAAATCGACCATGACTATGAAAGAGA
  TGGACTGAAAGAGAAAGTTTACCAAATGCTTCAGAAGTGGCTGATGCGGGAAGGCACCAAAGGGGCCACA
  GTGGGAAAGTTGGCCCAGGCACTTCACCAATGTTGCAGGATAGACCTGCTGAACCACTTGATTCGTGCCA
  GCCAGAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009068
• Insert Size: 1971 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009068.3, NP_033094.3
• RefSeq Size: 4512 bp
• RefSeq ORF: 1971 bp
• Locus ID: 19766
• UniProt ID: Q60855
• Cytogenetics: 13 14.01 cM
• Gene Summary: Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage (PubMed:12654725, PubMed:19590578). Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor (By similarity). Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (By similarity). Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules (By similarity). Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation (By similarity). Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death (By similarity). RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (By similarity).[UniProtKB/Swiss-Prot Function]