Vit (NM_028813) Mouse Untagged Clone

CAT#: MC220057

Vit (untagged) - Mouse vitrin (Vit), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vit
• Synonyms: 1700052E02Rik; 1700110E08Rik; 2810429K11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220057 representing NM_028813
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATTGTTGTTCCCACTATGAAGGCATCTGTTATTGAAGTGTTGCTTGTTTTGCTGGTAACTGGAA
  TACATTCAAATAAAGAAACGCCAAAGAAGACTAAAAGGCCCAAACTCACTGTGCCTCAGATCAACTGCGA
  TGTCAAAGCTGGAAAGATCATCAATCCTGAGTTCATGGTGAAATGTCCAGCGGGCTGCCAGGACCCCAAA
  TACCATGTTTACGGCACTGGCGTCTACGCGTCTTACTCCAGCGTGTGTGGCGCCGCAATTCACAGCGGTG
  TGCTGGATAATTCAGGAGGGAAAATACTTGTTCGGAAAGTTGCTGGGCAGTCTGGTTACAAAGGAAGCTA
  CTCGAATGGGGTTCAGTCTTTATCTTTACCCAGATGGCGAGAGTCCTTCATCGTCGCAGAAAGTAAGCCC
  CAAAAGGGTGTAGCCTACCCATCGACTCTTACATATTCATCATCGAAAACTGCAGCTGCTAAAGCAGGTG
  AGACCACAAAAGCCTATGAGAAGCCATCTATTCCAGGGACAACCATACAGCCTGTCACCCTGACACAGGC
  TCAAGCTACCCCTGTAGCTGAGGTCACCCACAGATCCACCTCAAAGCCATTTGCAGCATCTGTCACCAAC
  AGCCCCAGACCCCAACCTGTGGGCCACAGGAGTCAGGAGATGGAAGAGGTGGACGGGTGGAAGCCTGGAC
  CAGTCCTTTTAGATTCAGGATTTGTGCCAAAAGAAGAACTCAGCACACAGTCTTCGGAGCCAGTGCCGCA
  GGGAGATCCAAACTGCAAAATTGACTTGTCCTTCTTAATTGATGGGAGCACCAGCATCGGCAAGCGCCGC
  TTCCGGATCCAGAAGCAGTTCCTAGCGGACGTCGTCCAGGCTCTTGACATCGGCCCTGCCGGTCCCCTCG
  TGGGTGTTGTTCAGTACGGAGACAATCCTGCTACCCAGTTTAACCTCAAAACTCACATGAATTCTCAAGA
  TCTGAAGACAGCTATAGAGAAAATTACCCAGAGAGGAGGCCTTTCTAATGTAGGCCGTGCCATCTCCTTT
  GTAACCAAGACCTTCTTTTCCAAAGCCAATGGGAACAGAGGTGGTGCTCCCAATGTGGCTGTGGTCATGG
  TGGATGGCTGGCCCACAGACAAGGTGGAAGAGGTGTCACGAGTTGCCAGAGAGTCTGGGATCAACGTCTT
  CTTCATCACCGTCGAAGGGGCCGCCGAAAGGGACATACAGCATGTGGTGGAGCCCGGTTTTGCCAGCAAG
  GCAGTGTGCAGAACAAACGGGTTCTACTCCTTCAATGTGCAGAGCTGGCTCAGCCTTCACAAAACAGTGC
  AGCCCCTGGTGAAGCGGGTCTGCGACACGGACCGCCTAGCCTGCAGCAAGACCTGTTTAAACTCTGCGGA
  CATAGGCTTTGTCATCGATGGGTCTAGCAGCATGGGGACAAGCAACTTCCGCACGGTCCTGCAGTTCGTG
  GCCAACCTCAGCAAGGAGTTTGAGATTTCAGACACCGACACACGCGTCGGGGCCGTGCAGTACACCTACG
  AGCAGCGGCTCGAATTTGGGTTCGACAAGTATAACAGCAAAGCCGACATCCTCAGTGCCATCAGGAGGGT
  GGGGTACTGGAGCGGAGGCACCAGCACAGGGGCTGCCATCCAGTATGCCCTCGAACAGCTCTTCAAGAAG
  TCCAAACCGAACAAGAGGAAGGTAATGATCATTATCACTGACGGCAGGTCTTACGATGACGTGCGGATCC
  CAGCCATGGCTGCCTACCAGAAGGGGGTGATCACGTATGCAATAGGCATTGCGTGGGCTGCTCAGGACGA
  GCTGGAGGTAATGGCCACTCACCCCGCCAAGGACCATTCCTTCTTTGTGGATGATTTTGACAACCTCTAC
  AAAATTGCCCCCAGGATCATCCAGAATATTTGTACAGAGTTCAACTCACAGCCTCGAAACTGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_028813
• Insert Size: 1953 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028813.2, NP_083089.1
• RefSeq Size: 2737 bp
• RefSeq ORF: 1953 bp
• Locus ID: 74199
• UniProt ID: Q8VHI5
• Cytogenetics: 17 E3
• Gene Summary: Promotes matrix assembly and cell adhesiveness (PubMed:18757743). Plays a role in spinal cord formation by regulating the proliferation and differentiation of neural stem cells (PubMed:25331329).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).