Kcnc2 (NM_001025581) Mouse Untagged Clone

CAT#: MC220043

Kcnc2 (untagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 2 (Kcnc2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnc2
• Synonyms: AW047325; B230117I07; KShIIIA; Kv3.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220043 representing NM_001025581
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGATCGAGAGCAACGAGAGGGTGATCCTCAATGTCGGGGGTACCAGGCACGAAACCTACCGCA
  GCACCCTCAAGACCCTGCCTGGAACTCGCCTGGCCCTTCTTGCCTCCTCTGAACCTCAGGGCGACTGCCT
  GACTGCGGCCGGGGACAAGCTGCAACCGCTGCCCCCTCCGCTGTCTCCGCCGCCACGACCGCCTCCCTTG
  TCCCCTGTCCCCAGCGGCTGCTTCGAGGGCGGCGCAGGCAACTGCAGTTCGCACGGTGGCAACGGCGGCA
  ACGGCGGCAGCGACCACCCTGGGGGAGGCCGCGAATTCTTCTTCGATCGCCACCCAGGAGTATTCGCCTA
  TGTGCTCAATTACTACCGCACGGGCAAGCTGCACTGCCCCGCCGACGTGTGCGGGCCGCTCTTCGAGGAA
  GAGCTGGCTTTCTGGGGCATCGATGAGACCGACGTGGAGCCCTGCTGCTGGATGACCTACAGGCAGCACC
  GGGACGCGGAGGAGGCCCTGGACATCTTTGAGACACCCGACCTCATCGGGGGCGACCCTGGTGATGATGA
  GGACCTAGCGGCCAAGAGATTGGGCATTGAGGATGCTGCGGGGCTGGGAGGACCCGATGGCAAGTCAGGC
  CGCTGGAGGAAGCTGCAGCCTCGCATGTGGGCTCTTTTTGAGGACCCCTACTCATCTAGAGCCGCTAGGT
  TTATTGCTTTTGCTTCTTTGTTCTTCATTTTGGTTTCCATCACAACCTTTTGCCTGGAGACACATGAAGC
  TTTCAATATTGTTAAAAACAAGACGGAGCCCGTCATCAATGGCACCAGCCCGGTCCTCCAGTACGAAATC
  GAAACGGATCCCGCCCTGACGTACGTGGAAGGAGTATGTGTGGTGTGGTTTACGTTTGAATTTTTAGTCC
  GAATTGTTTTCTCACCCAATAAACTTGAATTCATCAAAAATCTCTTGAACATCATTGACTTTGTGGCCAT
  CCTCCCCTTCTACCTAGAGGTGGGACTCAGCGGGCTGTCCTCCAAAGCGGCCAAAGATGTGCTCGGCTTT
  CTCAGGGTGGTTAGGTTTGTGAGGATCCTGAGAATCTTCAAGCTCACCCGCCATTTCGTAGGTCTGAGGG
  TGCTCGGACATACTCTTCGGGCGAGCACCAACGAATTTTTGTTGCTGATCATCTTCCTGGCGCTGGGAGT
  TTTGATATTCGCTACGATGATCTACTACGCTGAGAGAGTAGGGGCTCAGCCCAATGACCCTTCAGCTAGT
  GAGCACACGCAGTTCAAAAACATCCCCATTGGTTTCTGGTGGGCCGTAGTGACCATGACTACCTTAGGTT
  ACGGGGATATGTACCCCCAAACATGGTCAGGGATGTTGGTGGGGGCCTTGTGTGCCCTGGCCGGAGTGCT
  GACAATAGCCATGCCTGTGCCTGTCATTGTCAACAATTTTGGAATGTACTACTCCTTGGCAATGGCGAAG
  CAGAAACTTCCAAGAAAGAGAAAGAAGCATATTCCTCCTGCCCCTCTGGCAAGCTCGCCTACATTTTGCA
  AGACAGAATTGAACATGGCTTGCAACAGTACCCAGAGTGACACATGTCTGGGCAAAGAAAACCGGCTTCT
  GGAACATAACAGATCAGTGTTATCAGGTGACGACAGTACAGGAAGTGAGCCGCCATTATCACCTCCAGAA
  AGGCTCCCTATCAGACGCTCTAGTACCAGAGACAAAAACAGAAGAGGGGAAACGTGTTTCCTGTTGACGA
  CAGGTGATTACACGTGCGCTTCTGATGGAGGAATCAGGAAAGCAAGCACACTAGAGCCCATGGAGAGTAC
  TGCACAGACTAAAGGAGACACAAGACCAGAAGCTCATTGGAATTGTGCGCACTTACTCAATTTTGGGTGT
  CCTACAGGAAGTTCATTTCCCACCCTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025581
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025581.1, NP_001020752.1
• RefSeq Size: 6196 bp
• RefSeq ORF: 1920 bp
• Locus ID: 268345
• Cytogenetics: 10 60.3 cM
• Gene Summary: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain. Contributes to the regulation of the fast action potential repolarization and in sustained high-frequency firing in neurons of the central nervous system (PubMed:10561420, PubMed:10414303, PubMed:11124984, PubMed:10903572, PubMed:11506885, PubMed:15317859, PubMed:15917463, PubMed:17761775, PubMed:21414897). Homotetramer channels mediate delayed-rectifier voltage-dependent potassium currents that activate rapidly at high-threshold voltages and inactivate slowly (PubMed:10414303). Forms tetrameric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (By similarity). Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNC1, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:10531438, PubMed:12000114). Channel properties may be modulated by either the association with ancillary subunits, such as KCNE1, KCNE2 and KCNE3 or indirectly by nitric oxide (NO) through a cGMP- and PKG-mediated signaling cascade, slowing channel activation and deactivation of delayed rectifier potassium channels (By similarity). Contributes to fire sustained trains of very brief action potentials at high frequency in thalamocortical and suprachiasmatic nucleus (SCN) neurons, in hippocampal and neocortical interneurons and in retinal ganglion cells (PubMed:10561420, PubMed:10903572, PubMed:11506885, PubMed:17761775). Sustained maximal action potential firing frequency in inhibitory hippocampal interneurons is negatively modulated by histamine H2 receptor activation in a cAMP- and protein kinase (PKA) phosphorylation-dependent manner (PubMed:10903572). Plays a role in maintaining the fidelity of synaptic transmission in neocortical GABAergic interneurons by generating action potential (AP) repolarization at nerve terminals, thus reducing spike-evoked calcium influx and GABA neurotransmitter release (PubMed:15917463). Required for long-range synchronization of gamma oscillations over distance in the neocortex (PubMed:22539821). Contributes to the modulation of the circadian rhythm of spontaneous action potential firing in suprachiasmatic nucleus (SCN) neurons in a light-dependent manner (PubMed:21414897).[UniProtKB/Swiss-Prot Function]