Ubqln2 (NM_018798) Mouse Untagged Clone

CAT#: MC219990

Ubqln2 (untagged) - Mouse ubiquilin 2 (Ubqln2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubqln2
• Synonyms: Chap1; Dsk2; HRIHFB2157; Plic-2; Plic2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219990 representing NM_018798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGAACGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCGCGGCCCTGCGGCGGCCCCAGGCGCGG
  CCAGCCCGCCGGCCGAGCCCAAAATCATCAAAGTCACTGTGAAGACCCCCAAAGAGAAGGAGGAGTTCGC
  GGTGCCCGAGAACAGCACCGTGCAGCAGTTCAAGGAAGCGATTTCCAAACGCTTCAAATCGCAGACCGAT
  CAGCTCGTGCTGATTTTCGCCGGAAAGATCCTGAAAGATCAAGATACCTTGATGCAGCATGGCATCCATG
  ATGGACTGACTGTTCACCTGGTTATCAAAAGCCAGAACCGTCCGCAGGGCCAGGCCACCACGCAGCCCAG
  CACGACTGCGGGAACGAGCACGACGACCACGACCACCACCACGGCGGCGGCGCCGGCGGCGACGACATCA
  TCGGCTCCCAGGAGTAGCTCCACACCTACCACCACGAATAGCAGCTCCTTTGGGCTGGGAAGCCTTTCCA
  GCCTTAGTAACCTGGGCTTGAACTCGCCCAACTTCACCGAGCTTCAGAACCAGATGCAGCAGCAGCTCCT
  GGCCAGCCCTGAGATGATGATCCAGATCATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCTGAT
  CTGATGAGGCAGCTCATCATGGCCAATCCACAGATGCAACAATTGATCCAGAGAAACCCAGAAATCAGCC
  ACCTACTGAACAACCCAGATATAATGAGGCAGACGCTGGAAATCGCCAGGAATCCTGCCATGATGCAAGA
  GATGATGCGAAATCAAGACCTGGCTCTCAGTAATCTCGAAAGCATCCCAGGTGGCTACAATGCTCTGCGG
  CGCATGTACACTGACATTCAAGAACCTATGCTGAATGCCGCACAGGAGCAGTTTGGGGGCAACCCGTTTG
  CCACGGTGGGGAGCAGTTCCACCTCAGGGGAAGGTACTCAGCCCTCCCGCACGGAGAATCGTGATCCGCT
  GCCCAATCCTTGGGCACCACCGCCAACAACCCAGACGGCTGCGACCACCACCACCACCACGACCACAAGC
  AGTGGCAGTGGCTCTGGCAGCAGCTCCAGCAGCACCACCGCCGGGAACACCATGGCTGCAGCTAATTACG
  TGGCTAGCATCTTCAGCACCCCAGGAATGCAGAGCCTGCTGCAGCAGATAACTGAGAATCCCCAGCTGAT
  TCAGAATATGCTGTCTGCGCCCTACATGCGGAGCATGATGCAGTCGCTGAGCCAGAATCCAGATATGGCG
  GCCCAGATGATGCTGAGTAGCCCGCTGTTCACTTCGAATCCTCAGCTGCAGGAGCAGATGCGTCCACAGC
  TCCCGAATTTCCTGCAGCAGATGCAGAATCCAGAAACCATCGCGGCCATGTCAAACCCGAGAGCGATGCA
  AGCGCTAATGCAGATCCAGCAGGGGCTACAGACTCTAGCCACTGAAGCACCTGGCCTCATTCCCAGCTTC
  GCTCCAGGTGTGGGGATGGGAGTGCTGGGAACCGCCATAACCCCTGTGGGCCCAGTCACTCCCATAGGGC
  CCATCGGCCCTATAGTTCCTTTCACCCCCATAGGCCCCATCGGGCCTATAGGACCCACTGGCCCTGCAAG
  CTCCCCCGGCTCCACCGGCACTGGCATCCCGCCTGCAACCACTGTGTCCAGCTCTGCACCTACTGAAACC
  ATAAGTCCAACCTCGGAATCTGGACCCAACCAGCAGTTCATTCAACAAATGGTGCAGGCTCTCACTGGAG
  GAAGCCCTCCACAGCCGCCGAATCCTGAAGTGAGATTTCAGCAGCAACTGGAACAGCTCAACGCCATGGG
  GTTCTTAAACCGCGAAGCAAACTTGCAGGCCCTAATAGCAACAGGAGGCGACATCAACGCGGCCATTGAG
  AGGCTGCTGGGCTCTCAGCCATCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_018798
• Insert Size: 1917 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018798.2, NP_061268.2
• RefSeq Size: 3343 bp
• RefSeq ORF: 1917 bp
• Locus ID: 54609
• UniProt ID: Q9QZM0
• Cytogenetics: X F3
• Gene Summary: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs) (By similarity). Links CD47 to vimentin-containing intermediate filaments of the cytoskeleton (PubMed:10549293).[UniProtKB/Swiss-Prot Function]