Synb (NM_173420) Mouse Untagged Clone

CAT#: MC219767

Synb (untagged) - Mouse RIKEN cDNA D930020E02 gene (D930020E02Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Synb
• Synonyms: D930020E02Rik; syncytin-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219767 representing NM_173420
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGCTTTTGGGTCCTCTGTTTCGTCCTTTTCCCCTCCTCCTTATCCTATCCGGAAAGCTGGATGC
  CCCTTGTAAACCTCACTCACCACATCCTACGTGATACCAACTCTTCCCTGTTTTCCAACTGTTGGGTCTG
  CTTGTCTACCCAAACCCAGCGGTCCTTAGCAGTCCCAGCCCCTCTGTCCATTTGGACAGATACACCCATG
  AAGCTTCATCTTACCTACTCAGTCAGGCCCTTCTCTGGCTCCTTTTCCATTAGCGACATTGAAAGACGCC
  TCCGTCTCTTCCGCCCACTGACTGCCTCCTATTCTTTCCACAATCCTGACAGAAGGGCGATTGCTTTTCT
  TCAACTCGTCAGCTCAACAGGCATATTTCGGATCATCACCCGGATAACCTCTGTGATATATCCCCATAAG
  GACCGTTTCTTCGAATCTGCCCAACGCCCTCTCTGGGGACCACTCTTTACTGAGACCGTGCTCAGGTCGC
  AGGCCCCACTCTGCATATCTCGCTTTTTCAAGGTCTCAGCATATGCCACTTTTGTAGGCAACCTCTCTGC
  CTCTCTCTGCAACTACACCATGCATATTTCACCTTCTACCAGTCATGAAAACCTAGATCTTTCCACCACC
  CATACGTTCAAACAGGCAATGAAAAGACCGGATGCCAAATGGAAAAACCCGCTCCGTTTTTCCGGGCCCC
  CCTCCCTCATCTTCTCGAAGCCGGCTTACTATCCCTGCCCAACAGACATCAAACACTGCCATACCTCTCC
  GGCCACTCCCTGGATGCACTGTCCTCAGGCTCCCTTCGGCACCTGCTATAACCTCACTTTATTTGAACCA
  GACAACTCAACCCACCCTGTTACCATGTCAGTGAACCCTACCCACTTCAAGGTCAAACTCCAGGGGCACA
  GAGACCCCTATCCGCTCTCCCATTACCAGCCCCTCACGGGAGCTGCCCTGTCTGGACAATATTCAGTCTG
  GGAGAACGAGATCACTGTCCAAGAAAACTGGGACATCACCTCCAACATTTTCTCACATCTTCTCAGCTTC
  TCGTACGCCTTCTGCCTCAACTCTTCAGGCGTTTTCTTCCTCTGCGGAACATCGACTTACATCTGCCTCC
  CAGCCAATTGGTCCGGTGTCTGTACCCTGGTCTTCCAATACCCGGATATTGAACTTCTCCCCAATAACCA
  AACGGTGCCTGTTCCCCTTTTTGCTTCAGTTCTTTCCTCAGACTCAGTTCTTCGCCCAAAGAGGTCCCCT
  CACCTCTTTCCCTTCCTTGCAGGCCTGGGTATCTCTTCTGCCCTTGGTACGGGGATAGCTGGCTTGGCCA
  CCTCGACTCTCTATTTCCAACAGCTTTCTAAGGTTCTTTCCGAAACCTTGGAAGAAATAGCTGCCTCTAT
  CACTACCCTCCAGAACCAAATAGACTCGCTCGCAGGTGTTGTTCTACAAAACCGCCGAGCTCTGGACCTC
  ATCACTGCTGAGAAAGGGGGCACCTGTCTCTTCCTCCAGGAAGAGTGCTGCTTCTACGTAAACCAGTCTG
  GAATAGTCCGGGACGCGGCAAGGAAACTCCAAGAACGAGCATCTGAACTCGGCCAGCATTCTGACTCTTG
  GGGACAGTGGCCTGACCTTGGACGTTGGTTGCCCTGGCTGACTCCCTTTCTGGGACCTCTTCTCTTCCTC
  TTCTTCCTACTGACATTTGGGTCTTGTCTTCTGAACTGCCTAACCCGTTTTGTGTCCCAGAGACTTGGCT
  CCTTTGTTCAAGACACTGCCAAAAGGCATGTGGACAGCATCCTCCAAAATTTCCAATATAAAAAACTGCC
  CCAAGACTCCCCAGATGAGGACACCATTCCTACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173420
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC137938, AAI37939
• RefSeq Size: 2593 bp
• RefSeq ORF: 1857 bp
• Locus ID: 239167
• UniProt ID: Q8BI41
• Cytogenetics: 14 D2
• Gene Summary: Many different endogenous retrovirus families are expressed in normal placental tissue at high levels, suggesting that endogenous retroviruses are functionally important in reproduction. This gene is part of an endogenous retrovirus provirus that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product plays a major role in placental development and trophoblast fusion. The protein has the characteristics of a typical retroviral envelope protein, including a cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. [provided by RefSeq, Apr 2015]