Txnrd1 (NM_001042523) Mouse Untagged Clone

CAT#: MC219721

Txnrd1 (untagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 1, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Symbol: Txnrd1
• Synonyms: T; TR; TR1; Trx; TrxR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219721 representing NM_001042523
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGTTGATGACTGCTGGCTGTACTTCCCAGCTTCTCGAGGTAGAACCTTTGTGCAGACTGTCTGGG
  TGGCACCCACTTGCCCCAACTGTTGCTGGTTTCCAGGTTTTCTCCCTCCAGTCCCCCGGCCACCACATGT
  GCCCCGTGTGCTGCTGAGGGGCCCTCGTGGGGCTGTGCTTCCTGCTTCACGTCCCTCCAAGACACTCCCC
  TCCTCATCCCAGACGCCCTGTCCTACTGACCCCTGTATCTGCCCTCCACCCTCCACACCTGATAGTAGGC
  AGGAAAAAAATACGCAATCTGAGCTGCCGAACAAAAAAGGCCAACTTCAAAAGCTGCCAACAATGAATGG
  CTCCAAAGATCCCCCTGGGTCCTATGACTTCGACCTGATCATCATTGGAGGAGGCTCAGGAGGACTGGCA
  GCAGCTAAGGAGGCAGCCAAATTTGACAAGAAAGTGCTGGTCTTGGATTTTGTCACACCGACTCCTCTTG
  GGACCAGATGGGGTCTCGGAGGAACGTGTGTGAATGTGGGTTGCATACCTAAGAAGCTGATGCACCAGGC
  AGCTTTGCTCGGACAAGCTCTGAAAGACTCGCGCAACTATGGCTGGAAAGTCGAAGACACAGTGAAGCAT
  GACTGGGAGAAAATGACGGAATCTGTGCAGAGTCACATCGGCTCGCTGAACTGGGGCTACCGCGTAGCTC
  TCCGGGAGAAAAAGGTCGTCTATGAGAATGCTTACGGGAGGTTCATTGGTCCTCACAGGATTGTGGCGAC
  AAATAACAAAGGTAAAGAAAAAATCTATTCAGCAGAGCGGTTCCTCATCGCCACAGGTGAGAGGCCCCGC
  TACCTGGGCATCCCTGGAGACAAAGAGTACTGCATCAGCAGTGATGATCTTTTCTCCTTGCCTTACTGCC
  CGGGGAAGACCCTAGTAGTTGGTGCATCCTATGTCGCCTTGGAATGTGCAGGATTTCTGGCTGGTATCGG
  CTTAGACGTCACTGTAATGGTGCGGTCCATTCTCCTTAGAGGATTTGACCAAGACATGGCCAACAAAATC
  GGTGAACACATGGAAGAACATGGTATCAAGTTTATAAGGCAGTTCGTCCCAACGAAAATTGAACAGATCG
  AAGCAGGAACACCAGGCCGACTCAGGGTGACTGCTCAATCCACAAACAGCGAGGAGACCATAGAGGGCGA
  ATTTAACACAGTGTTGCTGGCGGTAGGAAGAGATTCTTGTACGAGAACTATTGGCTTAGAGACCGTGGGC
  GTGAAGATAAACGAAAAAACCGGAAAGATACCCGTCACGGATGAAGAGCAGACCAATGTGCCTTACATCT
  ACGCCATCGGTGACATCCTGGAGGGGAAGCTAGAGCTGACTCCCGTAGCCATCCAGGCGGGGAGATTGCT
  GGCTCAGAGGCTGTATGGAGGCTCCAATGTCAAATGTGACTATGACAATGTCCCAACGACTGTATTTACT
  CCTTTGGAATATGGCTGTTGTGGCCTCTCTGAAGAAAAAGCCGTAGAGAAATTTGGGGAAGAAAATATTG
  AAGTTTACCATAGTTTCTTTTGGCCATTGGAATGGACAGTCCCATCCCGGGATAACAACAAATGTTATGC
  AAAAATAATCTGCAACCTTAAAGACGATGAACGTGTCGTGGGCTTCCACGTGCTGGGTCCAAACGCTGGA
  GAGGTGACGCAGGGCTTTGCGGCTGCGCTCAAGTGTGGGCTGACTAAGCAGCAGCTGGACAGCACCATCG
  GCATCCACCCGGTCTGTGCAGAGATATTCACAACGTTGTCAGTGACGAAGCGCTCTGGGGGAGACATCCT
  CCAGTCTGGCTGCTGAGGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042523
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042523.1, NP_001035988.1
• RefSeq Size: 3634 bp
• RefSeq ORF: 1842 bp
• Locus ID: 50493
• UniProt ID: Q9JMH6
• Cytogenetics: 10 C1
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (4) represents the use of an alternate upstream promoter, and contains 2 novel exons compared to variant 1. The encoded isoform (2) has a longer and distinct N-terminus, resulting from translation initiation at an alternate in-frame start codon, compared to isoform 1.