Gbp5 (NM_153564) Mouse Untagged Clone

CAT#: MC219463

Gbp5 (untagged) - Mouse guanylate binding protein 5 (Gbp5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gbp5
• Synonyms: 5330409J06Rik; Gbp5a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219463 representing NM_153564
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAGAGATTCACATGCCAGAACCCTTGTGCCTCATTGGGAGCACCGAGGGACACCTAGTAACTA
  ACCAGGAAGCCCTGAAGATTCTGTCTGCCATCACACAGCCAGTGGTGGTGGTAGCCATTGTGGGTCTTTA
  TCGCACAGGCAAATCCTACCTGATGAACAAGCTGGCTGGGAAGGAGAAGGGCTTTTCTGTTGGATCCACT
  GTACAGTCTCACACCAAGGGGATCTGGATGTGGTGTGTGCCTCACCCCCAAAAGCCAGACCACACTTTGG
  TTCTGCTTGACACTGAAGGCCTGGGAGATGTGGAGAAGGATGATAAAAAGAATGATACTCAGATCTTTGC
  ACTAGCAATCCTCCTCAGTAGCACCTTTGTATACAACACTATGAACAAAATTGACCAGGGGGCTATCGAC
  CTATTGCACAATGTGACAGAACTGACAGACTTGCTCCGGACAAGAAACTCCTCTGATTCTAATCAAACTG
  AGGGTGAGGGACCTGCTGACATGAGCTTCTTCCCAGACTTGGTGTGGACTCTGAGAGATTTCTTCCTGGA
  CCTGCAAGCCAATGGGCATGCCATCACATCAGATGAATATCTGGAGAATTCACTGAAGCTGAAGCAAGGT
  AGCGATGAAAGAACTCAAACATTCAATCTACCGCGGCTGTGCATACAGAAGTTCTTCCCAGTAAAGAAAT
  GCTTTGTTTTTGACGCTCCTGCGCTTGGAAGTAAGCTTTCCCAGCTTCCAACACTCAGCAACGAGGAGCT
  GAACTCAGATTTTGTGCAGGACCTTTCAGAATTCTGTTCACACATCTTCACCCAATCTAAGACCAAGACT
  CTTCCAGGAGGCATCCAGGTCAACGGACCTCGTCTAGAAAGCCTGGTGCTGACTTATGTCGATGCCATCA
  ACAGCGGGGCTCTGCCTTCCATAGAGAACACAGTAGTAACCTTGGCCCGGAGGGAGAACTCTGCAGCAGT
  TCAAAAGGCCATTGGTCACTACGATCAGCTGATGAGCGAGAAGGTGCAGCTGCCCACAGAGACCCTCCAG
  GAGCTGCTGGACCTGCACCGGACCTGTGAGCGAGAGGCCATAGAAATCTTCAGGAAGCATTCGTTCAAGG
  ATGAGGGTGAATTTTTCCAGAAAGAATTGGAGAGCCTACTAAGTGCAAAGCAGGATGAGATTTGTAAGAA
  GAACGCGGATGCTTCTGCAGCCCTCTGCTCAACCCTACTTGGGAGTATTTTTAAGCCTCTGGAACAAGAA
  GTGGCGCAGGAGTTCTATCATAAACCAGGAGGCCACAAGCTCTTCCTTCAGAGGATGGAACAGCTGAAGG
  CAAATTACCGTCAGCAGCCAGGGAAGGGAACACAGGCTGAGGAAGTGCTGCAGACCTATTTGAACGCCAA
  AGAAACAGTGAGCCGTACAATTCTACAAACAGACCAGGTTCTCACAGACAAGGAGATCCAGAGTAAAGCG
  GAACAAGAAAGAGCAGAGGCTGCCCGGCTCGAAGCACAGAGGTTGGAGGCTATTCGCATCCAGGAAGAGC
  AAAGGAAAGCAGAGATGGAGAGACAGCATCAAGAGCAATTGAGGCAAATAGCATTGGAGAAGGCAAGAGT
  TGCACAAGAGCAACAGTGGATCCTGAAGCAGAGAGCCCAGGAAGAGGCTGATAGAATCAAGGCAGAGCAG
  GAAGCTCAACTCAGAGCACTTCAACAGCAGCTCCAACACATGAGGGAAATGAACCACCACCGTAGACATC
  ATCATGACTGTGTTATAAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153564
• Insert Size: 1773 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153564.2, NP_705792.2
• RefSeq Size: 3021 bp
• RefSeq ORF: 1773 bp
• Locus ID: 229898
• UniProt ID: Q8CFB4
• Cytogenetics: 3 H1
• Gene Summary: As an activator of NLRP3 inflammasome assembly, plays a role in innate immunity and inflammation. Promotes selective NLRP3 inflammasome assembly in response to microbial and soluble, but not crystalline, inflammasome activating agents (PubMed:22461501). Hydrolyzes GTP, but in contrast to other family members, such as GBP1 and GBP2, does not produce GMP (By similarity).[UniProtKB/Swiss-Prot Function]