Sacm1l (NM_030692) Mouse Untagged Clone

CAT#: MC219440

Sacm1l (untagged) - Mouse SAC1 (suppressor of actin mutations 1, homolog)-like (S. cerevisiae) (Sacm1l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sacm1l
• Synonyms: mKIAA0851; SA; SAC1; Sac1p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219440 representing NM_030692
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCAGCCTACGAGCATCTGAAGCTGCATATTACACCTGAAAAGTTTTATGTGGAGGCTTGTG
  ATGATGGAGCAGATGATGTACTCATCATTGACCGAGTGTCCACAGAGGTCACCCTTGCAGTCAAAAAAGA
  TGTTCCCCCCTCTGCTGTTACAAGACCAATATTTGGGATACTGGGCACAATCCATCTGGTGGCAGGTAAT
  TATCTTGTTGTCATCACCAAAAAGATGAAAGTAGGTGAATGTTTTAATCATGCGGTCTGGAGAGCCACAG
  ATTTTGATGTTCTTTCTTATAAGAAGACAATGTTGCACTTGACTGATATTCAGTTACAAGATAATAAAAC
  TTTCCTAGCAATGCTAAACCATGTCTTGAGTATGGATGGATTTTACTTTTCAACAACATATGACTTGACT
  CATACTTTGCAGCGACTATCTAACACGAGTCCAGAATTCCAAGAAATGAGTCTCTTAGAAAGGGCAGATC
  AGCGGTTTGTATGGAACGGGCATCTGCTCAGGGAACTGTCTGCTCAGCCAGAGGTCCATCGGTTTGCTCT
  TCCAGTGTTGCATGGCTTTATTACTATGCACTCATGTTCTATTAATGGAAAATATTTTGATTGGATTCTC
  ATCTCCCGAAGGAGTTGTTTCAGAGCTGGTGTTCGTTATTATGTGAGAGGAATTGATTCTGAAGGCCACG
  CAGCTAACTTTGTAGAAACAGAGCAGATTGTGCACTACAGCGGGAACAGGGCTTCGTTTGTCCAGACTCG
  AGGATCAATACCTATTTTCTGGTCACAGAGACCAAATCTAAAGTACAAACCACATCCACAGATCAGCAAA
  GTAGCAAATCACATGGATGGTTTCCAGAGGCATTTTGATTCACAAGTAATTATTTATGGAAAGCAAGTAA
  TAATTAATCTGGTGAACCACAAGGGATCAGAGAAACCACTTGAGCAAACATTCGCAAACATGGTGTCCTC
  CTTGGGAAGTGGGATGATCAGATATATTGCCTTTGACTTCCATAAGGAGTGTAAAAATATGAGATGGGAT
  CGATTGAGTATTTTATTGGATCAAGTAGCAGAAATGCAGGATGAATTAAGTTATTTTTTAGTAGACTCTG
  CCGGCAAGGTGGTGACAAACCAGGACGGAGTTTTCCGCAGCAACTGCATGGATTGTCTAGACAGAACGAA
  TGTGATACAGAGTTTATTAGCTCGACGTTCCCTTCAGGCACAGCTGCAGAGGCTAGGAGTTTTGCATGTG
  GGACAGAAGCTTGAAGAGCAAGATGAATTTGAGAAGACTTACAAAAATGCCTGGGCCGATAATGCTAATG
  CTTGTGCCAAACAGTATGCGGGAACCGGTGCCTTGAAGACTGACTTTACAAGAACTGGAAAAAGAACTCA
  GTTGGGACTATTAATGGATGGCTTCAACTCATTATTACGGTATTACAAGAACAACTTTTCTGATGGATTT
  AGACAAGATTCCATAGACTTATTTCTTGGAAATTATTCAGTGGATGAATTAGAATCTCATAGCCCTTTAA
  GTGTTCCAAGGGACTGGAAATTCCTGGCGTTGCCTATCATCATGGTTGTTGCCTTTTCAATGTGCATCAT
  CTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACACTGGCATATGTCCTCTTCTGGGGAGTTGCAAGC
  ATTGGAACATTTTTTATTATTCTTTACAATGGCAAAGATTTTGTTGATGCTCCCAGACTGGTCCAGAAAG
  AAAAGATAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030692
• Insert Size: 1764 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030692.4, NP_109617.1
• RefSeq Size: 3433 bp
• RefSeq ORF: 1764 bp
• Locus ID: 83493
• UniProt ID: Q9EP69
• Cytogenetics: 9 74.08 cM
• Gene Summary: This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1).