Pak4 (NM_027470) Mouse Untagged Clone

CAT#: MC219418

Pak4 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 4 (Pak4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pak4
• Synonyms: 5730488L07Rik; AW555722; mKIAA1142
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219418 representing NM_027470
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGAAAGAAGAAGAAGCGGGTGGAGATCTCAGCCCCATCCAACTTCGAGCACCGTGTACACACAG
  GCTTTGATCAGCATGAGCAGAAGTTCACAGGGCTGCCCCGCCAGTGGCAGAGCCTGATTGAGGAATCAGC
  ACGCAGACCCAAGCCCCTGATCGACCCTGCCTGCATCACGTCCATCCAGCCTGGAGCCCCCAAGACCATC
  GTTCGGGGCAGCAAAGGTGCCAAGGATGGGGCTCTCACTCTGCTGCTTGACGAATTTGAGAACATGTCGG
  TGACGCGCTCCAACTCCCTACGCAGAGAGAGTCCACCACCACCTGCCCGTGCCCACCAGGAGAATGGAAT
  GCTGGAGGAGCGGGCAGCCCCGGCCAGAATGGCCCCTGACAAAGCTGGAAGCAGAGCCCGGGCCACAGGC
  CACAGTGAGGCAGGCAGTGGCAGTGGTGACAGACGGCGGGTGGGGCCAGAGAAAAGGCCCAAATCTTCGA
  GGGATGGTCCAGGAGGACCCCAGGAGGCCTCCCGCGATAAGCGCCCACTCTCTGGGCCTGATGTCAGCAC
  TCCTCAGCCTGGCAGTCTGACCAGCGGGACAAAACTAGCAGCTGGCAGGCCCTTTAACACATACCCACGG
  GCTGACACGGACCACCCACCCCGCGGTGCCCAGGGGGAGCCACATACCATGGCCCCTAATGGGCCTTCAG
  CCACAGGCCTGGCCGCTCCTCAGTCTTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGTGGTGCTCCCAG
  CCCAGGAGTTCTGGGTCCCCATGCCTCTGAGCCCCAGTTGGCCCCCCCAGCACGTGCCCTTGCTGCCCCT
  GCTGTACCTCCTGCCCCTGGGCCCCCTGGGCCTCGCTCACCACAGCGGGAGCCCCAACGAGTGTCCCATG
  AGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGGGACCCTCGTTCCTATCTAGACAACTTCAT
  CAAGATTGGTGAGGGCTCTACAGGTATTGTGTGCATTGCCACTGTACGCAGCTCAGGCAAACTGGTGGCC
  GTCAAGAAGATGGACTTGCGCAAGCAGCAAAGACGTGAACTGCTCTTCAATGAGGTGGTGATCATGCGGG
  ACTACCGGCACGAGAACGTGGTGGAGATGTACAACAGCTACCTGGTGGGTGACGAACTCTGGGTCGTCAT
  GGAGTTCCTGGAAGGCGGCGCCCTCACGGATATTGTCACCCACACCAGGATGAACGAGGAACAGATCGCC
  GCCGTGTGCCTGGCTGTGCTTCAGGCGCTGGCTGTGCTCCACGCCCAGGGTGTCATCCACCGTGACATCA
  AGAGTGACTCTATCTTGCTGACCCATGATGGCCGGGTGAAGCTGTCCGACTTCGGGTTTTGTGCCCAGGT
  GAGCAAGGAGGTGCCTCGGAGGAAGTCGCTGGTGGGCACCCCGTATTGGATGGCCCCGGAGCTCATCTCC
  CGCCTTCCCTATGGGCCAGAGGTGGATATCTGGTCACTGGGGGTGATGGTGATCGAGATGGTGGATGGGG
  AGCCCCCTTACTTCAACGAGCCACCCCTCAAAGCTATGAAGATGATCCGGGACAACCTCCCGCCCCGACT
  GAAGAACCTGCACAAGGCGTCACCGTCTCTGAAAGGCTTCTTGGATCGCCTGCTAGTGCGGGACCCGGCC
  CAGCGGGCCACTGCTGCCGAGCTGCTGAAGCACCCGTTCCTCACCAAGGCGGGGCCACCAGCCAGCATCG
  TGCCCCTGATGCGCCAGCACCGAACCAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027470
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027470.3, NP_081746.1
• RefSeq Size: 2898 bp
• RefSeq ORF: 1782 bp
• Locus ID: 70584
• UniProt ID: Q8BTW9
• Cytogenetics: 7 B1
• Gene Summary: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.[UniProtKB/Swiss-Prot Function]