Bbs1 (NM_001033128) Mouse Untagged Clone

CAT#: MC219406

Bbs1 (untagged) - Mouse Bardet-Biedl syndrome 1 (human) (Bbs1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbs1
• Synonyms: AI451249; D19Ertd609e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219406 representing NM_001033128
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCGTCTTCATCGGATTCTGACAGCGGGCGAGCTGAGAGCAACGAAGCCAATTCTAAATGGC
  TGGACGCACACTACGACCCCATGGCCAACATCCACACCTTCTCCTCCTGCCTGTCACTGGCAGATTTGCA
  TGGCGATGGGGAGTACAAGCTGGTGGTGGGAGACCTTGGCCCAGGTGGGCAGCAGCCCCGCCTGAAGGTG
  CTCAAAGGACCCACAGTGCTGACGGAGAGCCCACTGCCTGCCCTGCCAGCCTCGGCTGCCACCTTCCTCA
  TGGACCAACACGAGCCCAGGACACCTGCCCTGGCACTCGCCTCAGGGCCTTGTGTCTATGTTTATAAGAA
  CCTTAGACCCTACTTCAAGTTCAGCCTGCCCCAGTTACCTCCAAACCCTTTGGAACAGGACGTTTGGAAC
  CAAGCCAAAGAGGACCAGATTGACCCCTTAACCCTGAAGGAGATGCTGGAGGACATCAGGGAAAAGGCAG
  ACGTGCCTTTGTCTGTGCAGTCACTCAGGTTTCTGCAGCTGGAGCTGAGTGAAATGGAGGCGTTTGTGAA
  CCAGCACAAGTCCAAGGTTATCAAGCGCCAGACAGTCATCACCACCATGACCACCCTGAAGAAGAACCTG
  GCAGATGAGGACGCAGCGTCCTGTCTGGTGCTAGGCACTGAGAGCAAGGAACTCCTAGTGCTGGACCCTG
  AGGCCTTCACCATTTTGGCCAAGATGAGCCTGCCCAGTGTCCCTGTCTTCCTGGAGGTTTCCGGCCAGTT
  TGATGTGGAGTTCCGGTTGACTGCTGCCTGCCGAAATGGGAGCATCTATATCCTGAGAAGAGACTCCAAA
  CACCCCAAGTACTGCATCGAGTTGAGTGCCCAGCCAGTGGGGCTGGTCCGGGTACACAAGGTCCTGGTGG
  TGGGCAGCACCCAAGAGAGTCTGCATGGCTTCACCCACAAGGGTAAGAAGCTGTGGACAGTGCAGATGCC
  TGCAGCCATCCTGACCATGAACCTCCTGGAGCAGCGATCCCGAGGCCTGCAAGCTGTCATGGCTGCTCTG
  GCCAATGGGGAAGTCCGAATTTACCGAGACAAGGCCCTGCTCAATGTCATCCATGCCCCGGATGCAGTAA
  CCAGCCTGTGCTTTGGCCGATACGGGCGAGAAGACAACACCCTCATCATGACTACTAGAGGTGGCGGCCT
  GATCATCAAGATCCTGAAGCGCACAGCAGTGTTTGTGGAAGGGACGGGTGAGGTGGGCCCACCGCTAGCA
  CAGACCACAAAACTCAGTGTGCCCCGAAAGACCCGGCTGTATGTGGATCAGACCCTGCGGGAGCGGGAAG
  CTGGCACAGCCATGCACCGGACCTTCCAGACCGACCTCTACCTGCTGCGCCTGCGAGCTGCCAGAGCCTA
  TGTCCAGGCCCTGGAGTCCAGCCTGAGCCCCATGTCAACCACAGCTCGCGAGCCCCTCAAGCTGCATGCT
  GTGGTTCAAGGCCTGGGCCCCACCTTTAAGCTCACACTTCACCTGCAGAATACCTCAACGGCTCGACCCG
  TTCTGGGGCTGCATGTCTGCTTCCTGTACAACAAGGCGCTCTATGCCCTGCCTCAGGCTTTCTTTAAGGT
  TCCCTTGCTGGTACCAGGACTCAGTTACCCACTGGAAACCTTTGTGGAGAGTCTCAGTAGCAAGGGCATC
  TCAGACATGATCAAGGTGCTAGTGCTTCGGGAAGGCCAGAGTGCTCCCCTGCTGAGTGCCCACATCAACA
  TGCCTGTGAGTGAGGGTCTGGCAGCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033128
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033128.3, NP_001028300.1
• RefSeq Size: 5646 bp
• RefSeq ORF: 1782 bp
• Locus ID: 52028
• UniProt ID: Q3V3N7
• Cytogenetics: 19 A
• Gene Summary: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly (By similarity). Plays a role in olfactory cilium biogenesis/maintenance and trafficking and is essential for the localization of the BBSome complex in the olfactory sensory neurons cilia (PubMed:15322545, PubMed:28237838).[UniProtKB/Swiss-Prot Function]