Mbd1 (NM_013594) Mouse Untagged Clone

CAT#: MC219389

Mbd1 (untagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mbd1
• Synonyms: Cxxc3; PCM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219389 representing NM_013594
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGTCCTGGCAGGACTGCCCAGCCCTGGGCCCTGGCTGGAAACGCCGAGAGTCCTTTCGAAAGT
  CAGGGGCCTCGTTTGGACGCTCAGACATCTATTACCAGAGCCCCACAGGAGAAAAGATCCGAAGCAAAGT
  TGAGCTGACTCGGTACTTGGGCCCTGCATGTGATCTTACCCTGTTTGACTTCAGACAAGGCACCTTATGC
  CATCCCATTCCCAAGACTCATCCCTTGGCTGTCCCCAGCAAGAAGAAAAAGAAGCCTTCTAAACCAGCCA
  AGACTAAGAAACAGCAGGTTGGGCTGCAGCGAAGTGAGGTCAGGAGAGAGACTCCACAGGGGGAATATAA
  GGCCCCTACTGCCACAGCTCTAGCTTCACTATCTGTGTCTGCATCTGCGTCTTCGTCTGCATCTGCATCT
  GCGTCTTCGTCTGCATCTGCATCTGCATCTGCGTCTTCACATGCACCTGTGTGCTGTGAGAATTGTGGAA
  TCCACTTTTCATGGGATGGTGTCAAAAGGCAGAGACTTAAGACGTTGTGCAAAGATTGTCGAGCACAGAG
  AATCGCCTTCAACCGCGAACAGAGAATGTTTAAGCGAGTCGGCTGTGGGGACTGTGCAGCTTGCCTGGTG
  AAAGAGGACTGTGGGGTGTGCTCCACCTGTCGCCTGCAACTGCCCAGTGATGTGGCCTCAGGGCTTTACT
  GCAAGTGTGAGCGGAGACGATGCCTGCGGATTATGGAGAAGAGCCGAGGGTGTGGCGTGTGTCGGGGTTG
  TCAGACCCAAGAGGACTGTGGCCATTGCTGCATCTGTCTTCGCTCTCCCCGCCCTGGTCTCAAGCGCCAG
  TGGAGGTGTTTGCAACGGCGCTGTTTTTGGGGTAAACGTGATTCTAGTAAGAGAGGCTCGAAGGTAGCGT
  CTCAGCGTCACTCCCAAGCCCCGCCACTTCCTCCACATCCTGCATCACAGTACACAGAGCCCACAGAGCT
  GCACATCAGCGACATAGCACCCACATCACCTGCTGAGTTCATCTATTACTGTGTAGACGAGGACGAGGAC
  GAGCTAAAGCGGCTGCTGCCCAGTGCTGGGTCAGGGTCTGGGGAGGGAGCAGGATTGCGTCCATATCAGA
  CCCATCAGACCCATCAAAAGAGGCCTGCTTCTGCTCGACAACTCCAACTGAGCTCTCCCTTAAAGGCCCC
  TTGGGCTGTGGTCACAGCACCACCAGGCCCTGTCCGAGATTCAAGAAAGCAGCAAGCAGGTAGAGGCTCT
  GTACTGCCCCAGCCTGACACAGACTTTGTGTTTTTACAAGAGGGTACCAGCAGTGCCATGCAGATGCCTG
  GCACTGCTGCAGCTTGCACAGAAGTCCCAGTACAGGCGGCCCAGTGCTCTGCCCCGAGCTGGGTTGTGGC
  CTTACCCCAGGTGAAGCAAGAGACGGCAGATGCTCCAGAGGAGTGGACAGCAGTCACGACCTTCCTGACT
  TCCTCCACGCTGCAGTCTGGCTTCCCTAGCAAGGCTGCAGATCCAGACCTTTCACCTGTGAAACAAGAGC
  CCCCTGGCCCTGAAGAGGATGGAGAAGAGAAAAAAGATGATGTCTCTGAAACAACCCCAGCAGAGGAGAT
  AGGAGGGGTTGGCACGCCAGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCCTCCGAGATGCAGAA
  GCCTGGTTGCCAAGGCTACATAAACTGTTAGCAGTAAATGAAAAAGAGTATTTTACTGAGCTGCAGTTGA
  AAGAAGAAGTTTTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013594
• Insert Size: 1767 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013594.2, NP_038622.2
• RefSeq Size: 2672 bp
• RefSeq ORF: 1767 bp
• Locus ID: 17190
• UniProt ID: Q9Z2E2
• Cytogenetics: 18 50.7 cM
• Gene Summary: Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 can also repress transcription from unmethylated promoters.[UniProtKB/Swiss-Prot Function]