Nme8 (NM_181591) Mouse Untagged Clone

CAT#: MC219318

Nme8 (untagged) - Mouse thioredoxin domain containing 3 (spermatozoa) (Txndc3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nme8
• Synonyms: 1700056P15Rik; Sptrx-2; Txndc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219318 representing NM_181591
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCAAAAAGCGTGAAGTCCAGCTACAGTCAGTCGTCAATAGTCAGAACTTGTGGGATGAGATGT
  TGCTGAACAAAGGCTTAACAGTGATTGATGTTTACCAAGCCTGGTGTGGACCTTGCAAAGCCGTGCAAAG
  TTTATTCAGAAAACTAAAAAATGAACTGAACGAAGATGAGATTCTTCACTTCGTCGTTGCTGAAGCTGAC
  AACATTGTGACTCTCCAGCCATTTAGAGATAAATGTGAGCCCGTGTTTCTCTTTAGTCTTAATGGTAAAA
  TCATTGCAAAGATTCAGGGTGCAAATGCTCCACTTATCAATAGAAAAGTCATTACCTTGATAGATGAAGA
  GAGGAAAATTGTAGCAGGTGAAATGGATCGTCCTCAGTATGTTGAAATTCCACTAGTAGATGCAATCGAT
  GAAGAATATGGGGAAGTACAGTATGAAAGTGCTGCGGAAGTTTACAATATGGCAATTATCAAACCTGATG
  CTGTACTCATGAGAAAAAATATAGAAGTTAGGGAAAAAATAGCCAAAGAAGGATTTGTTATAGAAATACA
  AGAAAACCTGATTCTCCCTGAAGAGGTAGTGAGGGAATTCTACACTCATATAGCAGACCAGCCTGACTTT
  GAAGAGTTTGTCGTTTCTATGACAAATGGCCTCAGCTGTGTGCTCATTGTATCTCAAGAAGACTCCGAGG
  TTATTCAGGAAGAAACTCTCCCGCAGACTGATACAGAAGAAGAACCTGGCGTTTTGGAAGAGCCTCACGT
  TAGGTTTGCACCTGTGATGATAAAGAAGAAACGGGACAGTTTGCAAGAGTATATGGACCGACAGCATATG
  TCTGATTACTGCGATGTCGAGGACGATGCGGTTAAGGTCTCTAAGCTCATTGACATATTATTCCCTGATT
  TTAAAACTATGAAAAGCACGAATGTACAAACGACGCTAGCATTACTGCATCCAGACATCTGTGAGGAAGA
  GAAAGATGACGTGTTGAACGTTATTCACAATGAAGGGTTCACCATACTGATGCAGAGGCAAATCGTATTA
  TCAGAGGAAGAAGCAAGAACAGTGTGCAAGATCCATGAAAACGAAGAGTATTTTGATAATCTTATAGGGC
  ACATGACCAGTAATCACTCTTATGTCCTTGCTCTACGGAGGGAAAATGGTGTGGAATATTGGAAAACATT
  AATTGGGCCAAAAACGATTGAGGAAGCTTATGCATCTCATCCACAGAGTTTATGTGTACAGTTTGCTTCA
  GGGAATTTTCCTACCAACCAGTTCTACGGGAGCAGTTCAAAAGCAGCAGCTGAGAAGGAAATAGCGCATT
  TCTTCCCTCCCCAGAGCACACTTGCATTGATCAAGCCTCATGTGACACACAAAGAAAGAATGGAGATCCT
  GAAGACCATTAAAGAGGCAGGATTTGAGCTGACCCTGATGAAGGAAATGCACCTGACTCCAGAGCATGCA
  AACAAAATTTATTTCAAAATAACAGGAAAAGATTTTTATAAAAATGTATTGGAAGTCTTATCTTTGGGCA
  TGTCGCTAGTCATGGTTTTGACCAAGTGGAATGCTGTTGCAGAATGGAGGCGAATGGTTGGCCCAGTAGA
  CCCAGAAGAAGCAAAACTGCTCTCCCCAGAATCCCTCCGAGCCAAATATGGACTAGACATTTTGAGAAAT
  GCTGTCCATGGGGCGTCTAACTTTTCTGAAGCATCAGAAATCATTAGTAATGTGTTCACAGAGGGTAATC
  CTGAGAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181591
• Insert Size: 1761 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181591.3, NP_853622.2
• RefSeq Size: 2088 bp
• RefSeq ORF: 1761 bp
• Locus ID: 73412
• UniProt ID: Q715T0
• Cytogenetics: 13 7.0 cM
• Gene Summary: Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.