Eefsec (NM_023060) Mouse Untagged Clone

CAT#: MC219303

Eefsec (untagged) - Mouse eukaryotic elongation factor, selenocysteine-tRNA-specific (Eefsec), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eefsec
• Synonyms: mSelB; sec; Selb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219303 representing NM_023060
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCGGCGGGTAAATGTGAATGTGGGCGTGCTGGGTCACATCGACAGTGGCAAAACAGCGCTGG
  CGCGCGCGCTGAGCACCACGGCCTCCACTGCTGCCTTCGACAAGCAGCCGCAGAGCCGCGAGCGCGGCAT
  AACGCTCGACCTGGGCTTCTCCTGTTTTGTCGTGCCGCTGCCGGGGGCAGAACCCGGTTCTAGCGACACG
  CTGCTGCAGGTCACACTGGTCGACTGCCCCGGGCACGCCTCCCTCATCCGGACCATCATTGGCGGTGCCC
  AGATCATTGATTTGATGATGCTCGTGATTGATGTGACCAAGGGAATGCAGACACAGTCCGCAGAGTGCCT
  TGTGATTGGCCAGATCGCCTGCCAGAAGCTGGTTGTGGTGCTGAACAAAATAGACCTGTTAGCAGAAGGG
  AAGAGACAGGCAGCGATTGACAAAATGACCAAGAAAATGCAGAAGACTCTTGAGAACACCAAATTCCGGG
  GTGCACCGATTATTCCCGTGGCAGCCAAACCTGGGGGACCAGAAGCCCCTGAAACTGAAGCTCCACAGGG
  CATCTCAGAACTCATTGAGCTCCTGAAGTCCCAGATTTCCATCCCAACGAGAGACCCCTCTGGACCATTC
  CTTATGTCTGTGGACCACTGCTTCTCCATCAAGGGTCAAGGCACCGTGATGACTGGGACTATCCTCTCAG
  GCACTATCAGCCTTGGGGACAGTGTGGAGATCCCTGCCCTCAAGGTGGTGAAGAAAGTGAAGTCCATGCA
  GATGTTCCATACACCCGTCACCTCTGCCATGCAGGGGGACCGACTGGGCATCTGTGTCACCCAGTTTGAC
  CCCAAGCTGTTGGAGCGTGGGCTGGTATGTGCACCCGAGTCTCTGCACACTGTTCACGCAGCCCTCATCT
  CTGTGGAGAAGATCCCTTACTTCCGAGGACCCCTGCAGACCAAGGCCAAGTTCCATATCACCGTTGGCCA
  TGAGACAGTCATGGGTCGCACGCTGTTCTTCAGCCCTGCTCCTGACAGCTTTGACTTGGAGCCCGTGCTA
  GATTCCTTCGACCTTTCCCGAGAGTACCTCTTCCAAGAGCAGTACCTGTGCAAGGATTCCATGCCCACAG
  CCACAGAAGGTGATGACGAGGCGGACCCCAAAGCCGGCCACGCCCCAGGGGGCCACTGCCCCCGGCAGCA
  GTGGGCCCTGGTAGAGTTTGAGAAGCCAGTCACCTGCCCCCGACTCTGCTTGGTGATTGGCTCCAGACTA
  GATGCCGACATTCACACCAACACATGCCGGCTGGCCTTCCATGGTGTCTTGCTGCAAGGGCTGGAGGACA
  AGAACTACATTGAGAGCTTCCTGCCAGCACTGAGGGTTTACAAGCTGAAGCACAAGCATGGCCTTGTGGA
  GCGGGTAATGGATGACTACAGCGTGATTGGACGTTCCCTGTTCAAAAAGGAGACCAACATCCAGCTCTTT
  GTGGGGCTTAAGGTGCAGCTGTCCACAGGGGAACAGGGTATCATCGACAGTGCCTTCGGCCAAAGTGGCA
  AGTTCAAGATCCACATCCCTGGTGGCCTCAGCCCTGAGTCCAAGAAGATCCTGACACCTACACTCAAGAA
  GCGGAGCCGAGCCGGCCGTGGAGAGACCACCAAGCCAGAGGAGGGCACTGAACGGCCAGAGCCCATCCAG
  CCTGTGACACTCAACCTGTCTTTCAAGCGCTATGTCTTTGACACCCAGAAGCGCATGGTACAGACTCCCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023060
• Insert Size: 1752 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023060.3, NP_075547.1
• RefSeq Size: 2679 bp
• RefSeq ORF: 1752 bp
• Locus ID: 65967
• UniProt ID: Q9JHW4
• Cytogenetics: 6 D1
• Gene Summary: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.[UniProtKB/Swiss-Prot Function]