Eps15 (NM_001159964) Mouse Untagged Clone

CAT#: MC219270

Eps15 (untagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eps15
• Synonyms: 2410112D09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219270 representing NM_001159964
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTTAAAAAGCGATTCAGGATTAGGAGGATGGATAGCCATACCAGCTGTAGCTGATGTTCTCAGAT
  ACTCTTGCATTGTTTGCTGGTCTTCTAGGGAAAAGAACAATGTGGAGCAGGACCTTAAAGAGAAGGAAGA
  CACAGTTAAGCAGAGGACCAGTGAGGTTCAGGATCTTCAAGATGAAGTTCAAAGGGAGAGTATTAATCTA
  CAAAAACTGCAGGCCCAGAAGCAGCAGGTGCAGGAGCTCCTGGGTGAACTGGATGAGCAGAAAGCCCAGC
  TGGAGGAGCAGCTCCAGGAAGTCAGGAAAAAGTGTGCTGAGGAGGCCCAGCTGATTTCTTCCCTGAAAGC
  AGAAATAACTAGTCAAGAATCTCAGATCTCCAGTTATGAGGAAGAACTGTTGAAAGCTAGAGAAGAACTA
  AGTCGCCTACAACAAGAAACAGCACAATTGGAAGAAAGTGTGGAGTCAGGGAAGGCTCAGCTGGAACCTC
  TTCAGCAGCACCTACAAGAGTCACAACAGGAAATCAGCTCAATGCAAATGAGATTGGAAATGAAAGATCT
  GGAAACTGATAATAACCAATCAAATTGGAGCAGTAGCCCACAAAGCGTTCTTGTTAATGGTGCTACAGAT
  TACTGTAGCCTCAGCACCAGCAGCAGTGAAACAGCCAACTTCAACGAACATGCTGAAGGCCAAAACAACC
  TAGAGTCTGAACCCACACACCAGGAGTCCTCAGTAAGAAGTAGTCCTGAAATCGCACCTTCTGATGTGAC
  TGATGAAAGTGAGGCTGTGACTGTGGCTGGTAATGAGAAAGTTACTCCGAGATTTGACGATGACAAGCAC
  TCAAAAGAGGAAGATCCATTTAATGTAGAATCAAGTTCACTGACAGATGCAGTTGCAGATACAAACTTGG
  ATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCTTTCAAGGATGATCCTTTTGGAAAAATTGATCC
  ATTTGGTGGTGACCCTTTCAAAGGCTCAGATCCTTTTGCGTCTGATTGCTTCTTTAAGCAGACTTCTACT
  GATCCTTTTACCACTTCAAGTACGGACCCTTTCAGTGCATCCAGCAACAGCAGTAATACATCGGTAGAAA
  CTTGGAAGCACAATGACCCATTTGCTCCTGGTGGAACAGTTGTTGCTGCAGCGAGTGATTCAGCCACAGA
  CCCTTTTGCTTCTGTTTTCGGAAATGAATCATTTGGAGATGGATTTGCTGACTTCAGCACATTATCAAAG
  GTCAACAATGAAGATGCTTTTAATCCTACCATATCAAGTTCTACCAGCAGTGTGACCATTGCAAAACCTA
  TGTTAGAGGAAACAGCCAGCAAGAGTGAAGATGTGCCTCCAGCACTGCCGCCCAAAGTTGGCACTCCAAC
  AAGACCTTGCCCGCCACCCCCTGGGAAAAGACCCATCAACAAATTGGATTCTTCTGATCCCCTTAAACTG
  AATGATCCATTTCAGCCTTTCCCAGGCAATGATAGTCCCAAAGAAAAAGATCCTGATATGTTTTGTGATC
  CATTCACTTCTTCTACCACTACCAATAAAGAGGCTGACCCAAGCAATTTTGCTAACTTCAGTGCTTATCC
  CTCTGAAGAAGATATGATTGAATGGGCAAAAAGGGAAAGTGAGCGGGAAGAAGAACAGAGGCTTGCCAGA
  CTAAATCAGCAGGAGCAAGAAGACTTGGAACTGGCCATTGCACTTAGCAAATCTGAGATCTCAGAAGCAT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159964
• Insert Size: 1752 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159964.1, NP_001153436.1
• RefSeq Size: 4244 bp
• RefSeq ORF: 1752 bp
• Locus ID: 13858
• UniProt ID: P42567
• Cytogenetics: 4 C7
• Gene Summary: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks multiple exons at the 5' end and has a distinct 5' UTR and start codon, compared to variant 1. These differences result in a protein (isoform B) with a shorter and distinct N-terminus, compared to isoform A. Isoform B localizes to early endosome membranes.