Arsi (NM_001038499) Mouse Untagged Clone

CAT#: MC219251

Arsi (untagged) - Mouse arylsulfatase i (Arsi), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arsi
• Synonyms: BC020108
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219251 representing NM_001038499
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGCTCTCTCAGGATTCTCCCTGGTCAGCCTACTCAGCTTGGGCTACCTGTCCTGGGATTGGGCTA
  AGCCCGGCCTTGTGGCTGATGGGCCTGCCGAGGCTGGCGATCAGCCTTCGGTGGCTCCACCGCAGCCTCC
  ACACATTATCTTCATCCTCACCGACGACCAAGGGTACCACGACGTGGGATACCATGGCTCGGATATCGAG
  ACCCCAACGCTGGACCGGCTAGCAGCTGAGGGCGTCAAACTGGAGAATTATTACATCCAGCCCATATGTA
  CACCTTCGAGGAGTCAACTCCTCACCGGCAGGTACCAGATCCATACAGGATTGCAGCACTCCATTATCCG
  CCCACGGCAGCCCAACTGTCTGCCCCTGGACCAGGTGACGCTGCCCCAGAAGCTACAGGAGGCAGGCTAC
  TCCACCCACATGGTGGGCAAGTGGCATCTGGGCTTCTACCGGAAGGAGTGCTTGCCTACCCGCCGGGGCT
  TCGACACCTTCCTGGGTTCCCTCACAGGCAATGTGGATTACTACACCTACGACAACTGTGATGGCCCAGG
  GGTTTGTGGGTTTGACCTGCACGAGGGTGAGAGCGTGGCCTGGGGGCTCAGCGGCCAGTACTCCACTATG
  CTCTACGCTCAGCGTGCAAGCCACATCCTGGCCAGCCACAATCCCCAGAATCCCCTCTTTCTCTATGTGG
  CCTTCCAGGCAGTACACACGCCCCTACAGTCACCTCGAGAATACCTATACCGCTACCGCACGATGGGCAA
  CGTAGCACGGCGCAAGTACGCAGCCATGGTGACCTGCATGGACGAGGCTGTGCGTAACATCACCTGGGCC
  CTCAAGCGCTATGGTTTCTATAACAACAGTGTCATTATCTTCTCCAGTGACAATGGTGGCCAGACCTTCT
  CAGGGGGTAGCAACTGGCCCCTTCGAGGACGCAAGGGTACTTACTGGGAAGGTGGTGTGAGGGGCCTGGG
  TTTTGTCCACAGCCCACTGCTCAAGAAAAAGAGACGGACCAGTCGGGCCCTGGTCCATATCACAGACTGG
  TACCCAACACTGGTGGGTCTGGCAGGCGGTACTACATCAGCAGCTGATGGGCTGGATGGCTATGATGTAT
  GGCCAGCCATTAGTGAGGGCCGGGCCTCACCACGCACAGAGATCCTACACAACATCGACCCCCTCTACAA
  CCATGCCCGGCATGGATCCTTGGAGGGTGGCTTTGGGATCTGGAATACAGCAGTGCAGGCTGCTATCCGA
  GTTGGGGAGTGGAAGCTGCTCACTGGAGACCCAGGCTATGGTGACTGGATTCCACCTCAGACACTGGCCT
  CCTTCCCTGGCAGCTGGTGGAACCTGGAGCGGATGGCCAGCATCCGCCAGGCTGTGTGGCTCTTTAACAT
  CAGTGCTGACCCTTACGAACGAGAGGACCTGGCTGGCCAGCGACCCGATGTAGTCCGCACCCTTCTGGCT
  CGCCTGGCTGATTATAACCGTACTGCCATCCCCGTGCGTTACCCAGCTGCGAACCCTCGGGCCCATCCTG
  ACTTTAATGGGGGTGCTTGGGGGCCCTGGGCCAGTGAGGAGGAGGAAGAGGAGGAGGAGGAAGAGGAGGA
  AGGCAGAGCTAGAAGTTTCTCCCGGGGTCGCCGCAAGAAGAAATGCAAGATTTGCAAGCTTCGATCTTTT
  TTCCGTAAACTCAATACCAGGCTGATGTCCCACCGGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038499
• Insert Size: 1722 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038499.1, NP_001033588.1
• RefSeq Size: 1722 bp
• RefSeq ORF: 1722 bp
• Locus ID: 545260
• UniProt ID: Q32KI9
• Cytogenetics: 18 E1
• Gene Summary: Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.[UniProtKB/Swiss-Prot Function]