Rufy4 (NM_001170641) Mouse Untagged Clone

CAT#: MC219141

Rufy4 (untagged) - Mouse RUN and FYVE domain containing 4 (Rufy4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rufy4
• Synonyms: F930048N03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219141 representing NM_001170641
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACAACGGGACCATCCTCAAGATCAGCAGAAGCCTGAAAAATGCAGTCTCTGCCATCCTCCAGG
  GATATGGGGACGGGCAGGAGCCAGTGACAGAAACCAGCGCTGAGCTACACAGACTCTGTGGCTGCCTGGA
  GCTGCTACTGCAGTTTGATCAGAAAGAGCAGAGGAGCTTCCTGGGGGCCCGGAAGGATTACTGGGACTTC
  CTATTCACTGCCCTTCGACGGCACCGGGGATACACAGAGCAGATGAGCTTCATATGCTCACAGGACAAGC
  TGAAGACCTCACTAGGAAAAGGTCGTGCCTTTATCCGACTCTGCCTTGCCCGAGGCCAGCTGGCTGAGTC
  GATGCAGCTGTGCCTCCTGAACCCACAACTCACCAGGGAATGGTATGGCCCTCGGAGCCCTCTGTTGTGC
  GCTGAGCTCCAGGAAGACATCTTGGACTCTCTCTATGCCCTCAATGGAGTTGCCTTCAACTTGGACCTGC
  AGCGGCCAGACCTGGATGAAGCCTGGCCCATGTTCTCAGAGTCCCGCTGTTCCAGTCCCAGCCGGACCGG
  GAAAAGAAGACCCGGGAAACCCAAAGGTTTTCCAGAGGAGGTGAGATGCTCCAGGGGAGAGCAGCTGCAG
  GAACCAGACACTGGAGGAACCAGCTGTCTGCAGGATGCAACCAGAGAAGACCGCACACCTGACCTCTGTA
  AACCCTTGCAGCCAAGCCATCTTCCCACCTTTCTGGAAGAGAAGAGGGAGGATTCCAGAAGCCTCAGTTG
  TCCCCAGAGCACTTGGGAAACAGAAAGGGAAGGATTTCAGCTAGACCAGAAGGATGGAGGCCCAAAGCCC
  AGGAAATTCCTGGAAAACTCAACAGCCAGCATCCAGCAACAGAGGAGCAGGGCTAAGGATGTTAAAATGC
  AGCTGACAGGAAGGAAAGTTGAAGGAAAAGGAAGTCTGTCGGGGACAGAAGATCAGAGAACAACAGAAGG
  GATCCAGAAAAGGGCAGCAGATTGGGACCTTGGCCAGGGGCTGATGGCCCCCGGTCTTCAGGGAAGAGAA
  GATGCAGAGTTAGGATACAGGTGTGAGTGGAATCAGCCTGATGTTCTCAGGCAATCCTGGGTCCTGGGAA
  CAAAGAAAAGTTCTCCCACAGAGAAGCCACAAGAGTGGACAGGAGTGACCAGTGGAACCATGCAGGAGGA
  TGGGTCAGAAGTGCCCTTGCAGCAGGAGGTGATCAAGGACCCTGGGTATGGGCTCCAGCTGGCAAAGGAA
  CAGGCCCAGTGCCAGGAGCAGCTGCGGGCACAGGAAGCAGAACTTCAGGCGCTCCAGGAGCAGCTCAGCA
  GGTGTCAAAAAGAAAGAGCCCTGTTGCAAGTGAAGCTGGAGCAGAAGCAGCAGGAGGCTGAGAGGAGGGA
  CGCCATGTATCAGACAGAGCTTGAAGGGCAGAGGGACCTGGTCCAGGCCATGAAAAGGAGGGTGTTGGAA
  CTGATCCATGAGAAGGACCTTCAGTGGCAGAGACTCCAGCAGCTGTCTACTGTGGCCCCTGGACACTGCA
  TAGGCTGCAACAAGGTCTTCAGGCGTCTGTCTCGGCGGTATCCTTGCAGGCTCTGTGGAGGCCTGGTTTG
  CCACGCCTGCTCTGTAGACTACAAGAAGAGAGAGCGTTGCTGCCCAACCTGTGCCCAGCAGGAAGAAATC
  CAGGACACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170641
• Insert Size: 1692 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170641.1, NP_001164112.1
• RefSeq Size: 2336 bp
• RefSeq ORF: 1692 bp
• Locus ID: 435626
• UniProt ID: Q3TYX8
• Cytogenetics: 1 C3
• Gene Summary: Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.