Pdp1 (NM_001098230) Mouse Untagged Clone

CAT#: MC219138

Pdp1 (untagged) - Mouse pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdp1
• Synonyms: Gm1024; Ppm2c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_001098230.1
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCCAGTCAGAACGTCCAGTCTGCCACTGTTCTCTG
  ATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTCGTCCGTAACTGTGAACTGAGCAGAATCTATGG
  CACTGCATGTTACTGCCACCACAAACATCTCTGCTGTTCACCACCATACATTCCTCAGAATCGTCTGAGG
  TACACACCCCATCCTGCATATGCTACCTTTTGTAGGCCACGGGAGAACTGGTGGCAGTATACTCAAGGAA
  GGAGATACGCTTCTACACCGCAGAAATTTTACCTCACACCTCCACAAGTCAACAGCATCCTTAAAGCTAA
  TGAATACAGCTTCAAAGTACCAGAATTTGATGGCAAAAATGTCAGTTCCATTCTTGGATTTGACAGCAAT
  CAGCTGCCTGCAAATGCACCCATAGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGC
  TCTTGGGGGTTTTTGATGGTCATGCAGGCTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTCTATTA
  TATTGCTGTTTCCTTGTTGCCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAGTGGTCGGGCA
  CTGCTACCTATCCTTCAGTGGCACAAGCACCCCAATGATTACTTCAGTAAGGAGGCGTCCAAATTGTATT
  TCAACAGCTTGAGGACTTACTGGCAAGAACTCATAGACCTCAATACTGGAGAATCAGCTGATATTGATGT
  TAAGGAGGCTTTAATTAATGCTTTCAAGAGACTCGATAATGACATTTCATTGGAGGCTCAAGTTGGTGAT
  CCTAATTCTTTTCTAAATTACCTGGTGCTTCGGGTAGCATTTTCTGGGGCTACTGCTTGTGTGGCCCATG
  TAGATGGTGTTGACCTCCATGTGGCTAACACTGGTGATAGTAGAGCCATGCTAGGTGTGCAAGAAGAAGA
  TGGCTCTTGGTCAGCAGTCACACTCTCTAATGACCACAATGCTCAGAATGAAAGAGAACTAGAGCGTCTG
  AAACTGGAACACCCAAAAAATGAGGCCAAGAGCGTGGTAAAGCAGGACCGGCTGCTTGGCTTGCTGATGC
  CCTTTAGGGCTTTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTTCAAAAGAGAGTGATAGAGTCTGG
  CCCAGACCAGTTGAATGACAATGAATACACCAAGTTTATCCCTCCTAACTATCATACACCTCCTTATCTT
  ACTGCTGAGCCAGAGGTAACTTATCACAGATTAAGGCCACAGGATAAATTCCTAGTGTTAGCAACTGATG
  GGTTGTGGGAGACTATGCATAGACAGGATGTGGTTAGGATTGTGGGTGAATACTTAACTGGTATGCATCA
  CCAACAGCCAATAGCAGTTGGTGGGTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAAAGG
  AGAGCAAAGATGTCATCAGTGTTTGAGGATCAGAATGCAGCAACCCATCTCATTCGCCATGCTGTAGGCA
  ATAATGAATTTGGGGCTGTTGATCATGAACGACTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCG
  GATGTATAGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCATACCAAAAC
  CAGGAACAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098230
• Insert Size: 1692 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098230.1, NP_001091700.1
• RefSeq Size: 4233 bp
• RefSeq ORF: 1692 bp
• Locus ID: 381511
• UniProt ID: Q3UV70
• Cytogenetics: 4 A1
• Gene Summary: Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at an alternate start codon, compared to variant 4. Variants 2 and 5 encode the same isoform (b), which has a shorter and distinct N-terminus, compared to isoform d.