Lgsn (NM_153601) Mouse Untagged Clone

CAT#: MC219132

Lgsn (untagged) - Mouse lengsin, lens protein with glutamine synthetase domain (Lgsn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lgsn
• Synonyms: Gluld1; lengsin; Lgs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219132 representing NM_153601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGACGAAGGGGACCTCGCACAGGAGGACACAGCAAAAGATGAAGGCAATGTGACTGAAGGCAGCA
  GGATGAGCAAACTGAGAAGGGCAAGGAGAAAAGTCACCAAACCACATCTTTGCTCAATGGATGGGGAAGA
  AATCGCCAAGGCCAATTCCAGTGAGATGTCCAGAAACCAAATAGCAGATCTGAGCAAACCTGGCTCTGCT
  GAGTCTTGGTCCTCGCACAGTGCCAAAGATGCCTACCACCCGACACCAGTGGTAAAACCCTCGCTTCCCA
  GTGCCTTAGCCGGTGCTCCTGATGCTGAATTCAGCCCGAATACTGATCCCACCCGGTACAATGCTCAGAG
  TTTTAACCCGCCTCAACTTTCTGCTAGAATGAAACACATTAAACAAGAAATGGCCAAAAACCACCTCCAG
  TTTGTACGATTTGAAGCAACAGATTTGCACGGTGTCTCCAGATCTAAGAGCATCCCCGCACAGTTTTTTC
  AAGAAAAAGTGATCCACGGAGTTTTCATGCCCCGTGGGTATCTGGAATTGATGCCGAACCCTAAGGACAA
  TGAGGTGAATCACATAAGAGCCACATGCTTCAATAGTGATATAGTCCTCATGCCAGAGCTGTCGACCTTC
  AGAGTCTTGCCTTGGGCAGAGAGGACTGCGAGGGTGATTTGTGACACCTTCACAGTGACGGGCGAGCCTC
  TCCTGACATCCCCTAGGTACATCGCCAAGAGGCAGCTGCGCCAGCTGCAGGACGCCGGCTTCTGCCTGCT
  GTCCGCTTTCATCTATGATTTCTGCATTTTTGGTGTGCCCGAAGTGATCAATTCAAAGACCATATCTTTT
  CCTGCCTCCACATTACTAAGTAACCATGATCAGCCCTTCATGCAGGAACTGGTTGAGGGCCTGTATCAGA
  CAGGGGCGAATGTGGAGAGCTTCTCCTCGTCTACCAGGCCTGGGCAGATGGAGATCTGTTTTCTCCCAGA
  GTTTGGCATTAGCTCAGCTGATAATGCCTTTACCCTCAGAACAGGTCTCCAAGAAGTGGCCAGGAGATAT
  AATTACATAGCTAGCCTTGTAATTGAGACTGGATTCTGCAATTCAGGAATTTTGTCTCATAGCATCTGGG
  ATGTGGGCGGGAAGACAAATATGTTCTGCAGTGGTTCTGGGGTGGAGAGGCTCACGCTCACCGGGAAGAA
  GTGGTTGGCAGGCCTGCTGAAGCACTCTGCGGCTCTCAGCTGCCTGATGGCCCCTGCGGTCAACTGTCGC
  AAGCGCTACTGCAAGGACAGCAGAGACCTGAAGGACAGCGTGCCCACCACGTGGGGATACAATGACAACA
  GCTGTGCCTTGAACATCAAGTGTCATGGTGAAAAAGGCACCCAGATAGAAAATAAACTAGGGTCTGCAAC
  CGCGAACCCTTACCTGGTGTTGGCGGCCACTGTGGCAGCAGGCTTGGATGGTCTTCAGAGCAGTGATGGT
  GCTGCGGCTGGTTCCGATGAGAGTCAGGATCTCTATCAACCAGAACCTTCCGAGATCCCTTTGAAGATGG
  AAGATGCCCTTGCCGCCCTGGAGCAAGATGAGTGTCTGAAGCAGGCTCTGGGAGAGACTTTCATCCGCTA
  CTTTGTTGCCATGAAGAAGTATGAACTGGAAAACGAAGAAACAGATGCAGAGGGAAATAAATTCCTGGAG
  TATTTTATATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153601
• Insert Size: 1692 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153601.1, NP_705829.1
• RefSeq Size: 1911 bp
• RefSeq ORF: 1692 bp
• Locus ID: 266744
• UniProt ID: Q8CIX8
• Cytogenetics: 1 B
• Gene Summary: May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity.[UniProtKB/Swiss-Prot Function]