Bco1 (NM_021486) Mouse Untagged Clone

CAT#: MC219081

Bcmo1 (untagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bco1
• Synonyms: Bcd; Bcdo; Bcdo1; Bcmo; Bcmo1; beta-C; beta-CD; betaCM; betaCMOOX; Cm; CMO1; Cmoi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219081 representing NM_021486
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATAATATTTGGCCAGAATAAGAAAGAACAGCTGGAGCCAGTTCAGGCCAAAGTGACAGGCAGCA
  TTCCAGCATGGCTGCAGGGGACCCTGCTCCGAAACGGGCCCGGGATGCACACAGTGGGAGAGAGCAAGTA
  CAACCATTGGTTTGATGGCCTGGCCCTTCTCCACAGTTTCTCCATCAGAGATGGGGAGGTCTTCTACAGG
  AGCAAATACCTGCAGAGTGACACCTACATCGCCAACATTGAGGCCAACAGAATCGTGGTGTCTGAGTTCG
  GAACCATGGCCTACCCGGACCCCTGCAAAAACATCTTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT
  CCCCGACTTCACAGACAACTGTCTGATCAACATCATGAAATGTGGAGAAGACTTCTATGCAACCACGGAG
  ACCAACTACATCAGGAAAATCGACCCCCAGACCCTAGAGACCTTGGAGAAGGTTGATTACCGGAAGTATG
  TGGCGGTAAACCTGGCTACCTCGCACCCTCATTATGACGAGGCTGGGAATGTCCTTAACATGGGCACATC
  CGTCGTGGACAAAGGGAGGACAAAATACGTGATATTTAAGATCCCTGCCACAGTGCCAGACAGCAAGAAG
  AAAGGGAAGAGTCCCGTGAAGCACGCGGAAGTTTTCTGCTCCATTTCCTCCCGCTCGTTGCTCTCTCCCA
  GCTACTACCACAGCTTTGGTGTCACGGAGAACTATGTGGTGTTTCTGGAGCAGCCTTTTAAGTTGGATAT
  CCTCAAGATGGCCACCGCATACATGAGGGGAGTGAGCTGGGCTTCCTGTATGTCATTCGACAGGGAGGAC
  AAGACATACATTCATATCATCGACCAGAGGACCAGGAAGCCTGTGCCTACCAAGTTCTACACAGATCCCA
  TGGTGGTCTTCCATCATGTCAATGCCTACGAGGAGGACGGCTGTGTGCTGTTTGATGTGATCGCCTATGA
  GGACAGCAGCCTCTATCAGCTCTTCTACCTGGCCAACCTGAACAAGGACTTCGAGGAGAAGTCCAGGCTG
  ACCTCAGTGCCTACCCTCAGGAGGTTTGCTGTGCCCCTCCATGTGGACAAGGATGCAGAAGTGGGCTCAA
  ATTTAGTCAAGGTGTCATCTACAACTGCAACAGCCCTGAAGGAGAAAGACGGCCATGTCTATTGCCAGCC
  CGAGGTCCTCTACGAAGGCCTAGAGCTCCCTCGGATAAATTATGCTTACAACGGGAAGCCATATCGCTAC
  ATCTTTGCAGCTGAAGTACAGTGGAGTCCAGTCCCAACCAAGATACTGAAATATGACATTCTCACAAAGT
  CCTCCTTAAAGTGGTCTGAGGAGAGCTGCTGGCCAGCAGAGCCTCTGTTTGTTCCCACGCCAGGTGCGAA
  GGATGAAGATGATGGAGTCATTTTATCAGCCATCGTCTCTACGGATCCCCAAAAGCTGCCTTTTTTACTC
  ATTCTGGATGCCAAAAGTTTTACGGAACTGGCTCGCGCCTCTGTTGATGCGGACATGCACCTGGACCTTC
  ATGGTTTATTTATCCCAGATGCAGACTGGAATGCAGTGAAGCAGACTCCAGCTGAAACGCAAGAGGTTGA
  AAACTCAGATCATCCCACAGATCCGACAGCACCAGAACTGAGCCACAGTGAAAATGACTTCACAGCGGGT
  CATGGTGGCTCAAGTCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021486
• Insert Size: 1701 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021486.3, NP_067461.2
• RefSeq Size: 2327 bp
• RefSeq ORF: 1701 bp
• Locus ID: 63857
• UniProt ID: Q9JJS6
• Cytogenetics: 8 E1
• Gene Summary: Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.