Rnf168 (NM_027355) Mouse Untagged Clone

CAT#: MC219044

Rnf168 (untagged) - Mouse ring finger protein 168 (Rnf168), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf168
• Synonyms: 3110001H15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219044 representing NM_027355
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGGCTGCACCTAAAACCTCCATCCCTTCCTTAGCCGAATGTCAGTGCGGGATCTGTATGGAAA
  TCCTCCTAGAGCCTGTAACCCTACCTTGCAACCACACGCTCTGTAACCCATGCTTCCAGTCCACTGTTGA
  AAAGGCCAATCTATGCTGTCCCTTCTGTCGTCGCCGGGTCTCTTCGTGGACTCGGTACCATACCCGAAGA
  AATTCTCTGGTCAATACAGACCTGTGGGAGATTATTCAAAAACACTATGCAAAGGAATGCAAGCTTAGAA
  TCTCTGGACAAGAATCAAAGGAAATCATTGATGAGTGCCAGCCAGTTCGTCGGCTCAGTGAACCTGGGGA
  GTTGAGGCGAGAATATGAAGAGGAGATAAGCAGGGTGGAGGCTGAGCGACAAGCCAGCAAGGAAGAGGAA
  AACAAAGCCAGTGAGGAGTACATCCAGAGATTGTTGGCTGAGGAGGAGGAGGAGGAGAAAAGACAGAGAG
  AAAAAAGAAGAAGTGAGATGGAAGAACAGCTGAGAGGCGATGAAGAACTGGCTAGGAGCCTGAGCACCAG
  TATCAACAGCAACTATGAGAGAAATACCTTGGCTTCTCCTTTGAGTTCCAGAAAATCAGATCCAGTCACA
  AACAAGTCCCAGAAGAAAAATACAAGTAAACAAAAAACCTTTGGAGATATTCAAAAATATTTGTCACCTA
  AGTTGAAGCCTGGGACCGCCTTGGCATGTAAGGCTGAGCTTGAGGAAGACATCTGCAAGTCTAAGGAAAC
  TGATAGGAGTGACACGAAGAGCCCTGTGTTGCAAGACACAGAAATTGAAAAAAATATACCAACACTTTCT
  CCTCAGACCTGCCTGGAAACTCAAGAACAAGGTTCGGAGTCTTCTGCAGGGATACCCGGGCCACAGTTAT
  GTGTAGGAGACACTAAAGAGTCCCTTGAGGGAAAAGTTGAAACAGTATCAACCAGTCCTGATGATTTATG
  TATTGTAAATGATGATGGACCTAGAGCCACAGTTTTTTACTCTAATGAAGCTGCAGTTAACTCTTCTAGT
  AAAATAGAAAATGAAGAATACTCTGTGACAGGTGTGCCCCAGTTAACTGGGGGCAACAGAGTTCCAACAG
  AGAGTAGAGTGTATCACTTACTAGTTGAAGAAGAGATTTCCGACAGAGAAAACCAGGAATCTGTATTTGA
  AGAAGTCATGGATCCATGTTTTTCTGCAAAAAGAAGGAAAATATTCATCGAATCCTCCTCAGATCAAGAA
  GAAACAGAAGTGAATTTTACACAAAAACTGATAGATTTGGAACATATGCTTTTTGAGAGACATAAGCAAG
  AAGAGCAGGACAGATTGTTAGCATTACAACTTCAGAAAGAGGTAGATAAAGAGCAAATGGTGCCAAACCG
  GCAGAAAGGATCCCCAGATCAGTACCAGCTACGCACACCCTCACCCCCAGACAGGCTGCTGAATAGACAA
  AGGAAGAATTCCAAAGATAGGAACTCCCTACAGCAAACTAATGCAGATCACTCAAAATCTCCGAGGAACA
  CAAAAGGTGACTATTGGGAGCCCTTTAAAAACACATGGAAGGATTCAGTTAATGGAACAAAGATGCCAAC
  TTCTACTCAAGATAATTGTAATGTATCTAAAAGTGCCTATACCGTACAGCATAGAAAGTCCCAGAGAAGC
  ATTGTTCAAATGTTTCAGAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027355
• Insert Size: 1704 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027355.2, NP_081631.2
• RefSeq Size: 4468 bp
• RefSeq ORF: 1704 bp
• Locus ID: 70238
• UniProt ID: Q80XJ2
• Cytogenetics: 16 B3
• Gene Summary: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).[UniProtKB/Swiss-Prot Function]