Gpr161 (NM_001081126) Mouse Untagged Clone

CAT#: MC219021

Gpr161 (untagged) - Mouse G protein-coupled receptor 161 (Gpr161), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpr161
• Synonyms: Gm208; Gm208Gpr; vl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219021 representing NM_001081126
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGCACCACACCACACACACTCTCTTGGCTGTATTTCCTGTCGTCCAGCATGCTCTGCTCACCG
  CATCCCGAGGGGCACTGACCATGAGCCTCAACTCCTCCCTCAGCTACAGGAAGGAACTGAGCAACCTCAC
  TGCAACAGAGGGTGGTGAAGGGGGTGCCGTCTCTGAGTTCATCGCCATCATCATCATCACTGTCCTCGTC
  TGCCTGGGCAACCTGGTCATTGTAGTCACCTTGTACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGT
  TCGTCTTCAGCCTGACCTTGTCCAACTTCCTGTTGTCGGTGCTGGTGCTGCCGTTCGTGGTGACTAGCTC
  CATCCGGAGGGAATGGATTTTCGGCGTGGTTTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGC
  TCAGCCAGCATGCTCACGCTTGGGGTCATTGCCATCGATCGCTACTACGCCGTCCTGTATCCAATGGTGT
  ACCCCATGAAGATCACAGGGAACCGAGCTGTGATGGCTCTCGTCTACATCTGGCTCCACTCTCTCATTGG
  CTGTCTGCCGCCCCTATTTGGTTGGTCATCGGTGGAGTTTGATGAGTTCAAGTGGATGTGTGTGGCCGCG
  TGGCACCAGGAACCCGGCTACACCATTTTCTGGCAGATCTGGTGTGCCCTGTTCCCCTTTCTCATCATGC
  TAGTGTGCTACGGTTTCATCTTCCGGGTGGCCAGGGTCAAGGCCCGAAAGGTGCACTGTGGCACGGTGGT
  CACTGTGGAGGAGGACTCTCAGAGGAGCGGGAGGAAGAATTCTAGTACCTCCACTTCCTCCTCCGGCAGT
  AGGAGGAATGCCCTTCAAGGAGTGGTCTATTCAGCTAACCAGTGCAAAGCCCTCATCACCATCCTGGTGG
  TCATTGGCGCCTTCATGGTCACCTGGGGCCCCTACATGGTTGTCATTACCTCAGAGGCACTCTGGGGGAA
  GAACTGTGTCTCCCCAACCCTGGAGACTTGGGCCACATGGCTGTCCTTTACCAGTGCCATCTGCCACCCT
  CTGATCTACGGACTCTGGAACAAGACTGTGCGCAAGGAGCTCCTGGGCATGTGCTTTGGGGACCGTTATT
  ACCGGGAATCCTTTGTGCAGCGACAGAGGACCTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACTT
  GGGTCTGTCCCCACATCTCACGGCGCTCATGGCAGGCGGACAGTCCCTGGGACACAGCAGCAGCACCGGT
  GACACAGGCTTCAGCTACTCTCAGGATTCAGGGACGGATGTGATGCTGTTGGAAGATGGCACTTCTGAGG
  ACAACCCTCCCCAGCACTGCACTTGCCCACCCAAGAGGAGGAGCTCCGTGACATTTGAGGACGAAGTGGA
  ACAGATCAAAGAGGCCGCCAAGAACTCTCTTCTTCATGTGAAAGCTGAAGTACACAAATCCTTGGACAGT
  TACGCAGCCAGCTTGGCTAAAGCCATTGAGGCTGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTCTGC
  CAGGGGTCTTGTTCACAGCACGGACCGTCCCAGGGGCTGGCTTTGGGGGCCGTCGAGGCAGCAGAACTCT
  TGTGAATCAGAGGCTGCAGCTACAGAGCATCAAAGAAGGCAATGTCTTAGCTGCAGAACAGAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081126
• Insert Size: 1677 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081126.1, NP_001074595.1
• RefSeq Size: 1943 bp
• RefSeq ORF: 1677 bp
• Locus ID: 240888
• UniProt ID: B2RPY5
• Cytogenetics: 1 72.64 cM
• Gene Summary: Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.