Dennd6a (NM_145969) Mouse Untagged Clone

CAT#: MC218999

Dennd6a (untagged) - Mouse family with sequence similarity 116, member A (Fam116a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dennd6a
• Synonyms: Fam116a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218999 representing NM_145969
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCCGGGCCCGGCGGTCTTTGGGCCCGGTTCCCGCGGGTCGTTGGACGAGGCCGGGGCTGAGG
  GGCGCGAGGCGGCGGCCCTTGCGGCGGCGGGAGTCGCGCTGGAGGACGAGGAGGAGGACGATGGCCGCCG
  GGGCCTGCTGCGCTGGGACGGCTTCTCGGCCTGGCTACACTGCGTGTGTGTGGTGGGCTTCGACCTGGAG
  CTGGGCCAGGCGGTGGAGGTGATATATCCTCAGCATTCCAAACTTACTGATAAAGAAAAAACCAATATTT
  GCTACTTGTCTTTTCCAGACTCAAATTCAGGTTGTCTTGGAGATACCCAGTTTTGTTTTAGATTCCGACA
  GTCTTCTGGGAGAAGGGTGTCACTACACTGTCTTCTTGATGAATTTGACAAAGATTTACCAGTTTACTTA
  AAGAAAGATCCTGCATATTTTTATGGATATGTGTATTTTCGACAAGTTCGAGATAAAACTCTTAAAAGAG
  GCTACTTTCAAAAGTCATTGGTTTTGATCAGCAAACTACCTTATATTCATTTTTTTCACACCGTACTCAA
  ACAGATAGCACCAGAGTATTTTGAAAAAAATGAACCTTACTTGGAAGCAGCTTGTAATGATGTTGACCGA
  TGGCCTGCCCCAGTGCCAGGGAAAACGCTGCACCTGCCTATCATGGGACTAGTAATGAAGGTTCGGATTC
  CCACGTGTCATGACAAGCCTGGGACCACGCAGATGGTGCAGTTAACTCAGCAGGCAGATACACACACATC
  TATTATTTTGCCTACTGTTCACGAGGTGGATCTTTTCAGGTGTTTCTGCCCAGTTTTTCTTCACAGTCAG
  ATGCTCTGGGAGTTGGTGCTCTTGGGAGAGCCCCTGGTGGTCATGGCGCCATCGCCGTCAGAATCTTCAG
  AAACTGTACTGGCTCTTGTTAACTGTATCTCTCCATTAAAGTACTTTAGTGATTTTCGGCCTTACTTCAC
  GATTCATGATAGTGAATTCAAAGAATATACTACCCGTACTCAAGCTCCGCCCTCAGTCATCTTAGGAGTA
  ACCAACCCCTTTTTTGCTAAAACACTACAGCACTGGCCACACATTATTCGAATAGGAGATCTTAAACCTG
  CAGGTGAAATTCCTAAGCAAGTTAAAGTGAAAAAGCTGAAGAACCTAAAAACTCTGGATTCTAAACCTGG
  AGTTTATACTTCTTACAAGCCATATCTAAACAGAGATGAGGAGATCATAAAACAACTTCAGAAGGGTATA
  CAGCAGAAGCGTCCTTCTGAGGCCCAAAGTGTTATTCTCCGGCGCTATTTTTTGGAACTAACACAAAGTT
  TCATCATTCCATTAGAAAGATATGTGGCAAGCTTGATGCCTTTGCAGAAAAGTATTTCTCCTTGGAAGAG
  TCCACCCCAGTTACGGCAGTTCCTTCCAGAAGAATTTATGAAAACACTTGAAAAAACAGGGCCTCAGCTC
  ACCTCTGGAATAAAGGGCGACTGGATTGGACTTTACCGGCAGTTTCTAAAGTCTCCAAATTTTGATGGCT
  GGTTCAAGACCCGGCGGAAAGAAATGACTCAAAAATTGGAGGCACTTCATCTAGAAGCTCTTTGTGAAGA
  GGTCAGAAAAAAGTGTATTAGTACATGCAAGTGTTCAGAGTACAAAAGTATTCTTAGAGGCTTAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145969
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145969.4, NP_666081.1
• RefSeq Size: 2684 bp
• RefSeq ORF: 1680 bp
• Locus ID: 211922
• UniProt ID: Q8BH65
• Cytogenetics: 14 A3
• Gene Summary: Guanine nucleotide exchange factor (GEF) for RAB14. Component of an endocytic recycling pathway that is required for the control of ADAM10 transport, shedding of N-cadherin/CDH2 by ADAM9 or ADAM10 and regulation of cell-cell junctions. Required for RAB14 recruitment to recycling endosomes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a shorter and distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.